Insulin-Induced Cardiomyocytes Hypertrophy That Is Prevented by Taurine via β-alanine-Sensitive Na<sup>+</sup>-Taurine Symporter
Although insulin-induced cardiac hypertrophy is reported, very little information is available on the hypertrophic effect of insulin on ventricular cardiomyocytes and the regulation of sodium and calcium homeostasis. Taurine is a non-essential amino acid synthesized by cardiomyocytes and the brain a...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/13/11/3686 |
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| author | Ashley Jazzar Danielle Jacques Ghassan Bkaily |
| author_facet | Ashley Jazzar Danielle Jacques Ghassan Bkaily |
| author_sort | Ashley Jazzar |
| collection | DOAJ |
| description | Although insulin-induced cardiac hypertrophy is reported, very little information is available on the hypertrophic effect of insulin on ventricular cardiomyocytes and the regulation of sodium and calcium homeostasis. Taurine is a non-essential amino acid synthesized by cardiomyocytes and the brain and is present in low quantities in many foods, particularly seafood. The purpose of this study was to investigate whether chronic exposure to insulin induces hypertrophy of ventricular cardiomyocytes that are associated with changes in Na<sup>+</sup> and Ca<sup>2+</sup> homeostasis and whether taurine pre-treatment prevents these effects. Our results showed that chronic treatment with insulin leads to cardiomyocyte hypertrophy that is associated with an increase in basal intracellular Na<sup>+</sup> and Ca<sup>2+</sup> levels. Furthermore, long-term taurine treatment prevents morphological and ionic remodeling induced by insulin. In addition, blocking the Na<sup>+</sup>-taurine co-transporter prevented the taurine antihypertrophic effect. Finally, the insulin-induced remodeling of cardiomyocytes was associated with a decrease in the ratio of phospho-CREB (pCREB) to total cAMP response element binding protein (CREB); taurine prevented this effect. In conclusion, our results show that insulin induces ventricular cardiomyocyte hypertrophy via downregulation of the pCREB/tCREB level and that chronic taurine treatment prevents this effect. |
| first_indexed | 2024-03-10T05:11:38Z |
| format | Article |
| id | doaj.art-23c66286d07c43368d28a00cb548fe24 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T05:11:38Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-23c66286d07c43368d28a00cb548fe242023-11-23T00:44:20ZengMDPI AGNutrients2072-66432021-10-011311368610.3390/nu13113686Insulin-Induced Cardiomyocytes Hypertrophy That Is Prevented by Taurine via β-alanine-Sensitive Na<sup>+</sup>-Taurine SymporterAshley Jazzar0Danielle Jacques1Ghassan Bkaily2Department of Immunology and Cell Biology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC J1H 5N4, CanadaDepartment of Immunology and Cell Biology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC J1H 5N4, CanadaDepartment of Immunology and Cell Biology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC J1H 5N4, CanadaAlthough insulin-induced cardiac hypertrophy is reported, very little information is available on the hypertrophic effect of insulin on ventricular cardiomyocytes and the regulation of sodium and calcium homeostasis. Taurine is a non-essential amino acid synthesized by cardiomyocytes and the brain and is present in low quantities in many foods, particularly seafood. The purpose of this study was to investigate whether chronic exposure to insulin induces hypertrophy of ventricular cardiomyocytes that are associated with changes in Na<sup>+</sup> and Ca<sup>2+</sup> homeostasis and whether taurine pre-treatment prevents these effects. Our results showed that chronic treatment with insulin leads to cardiomyocyte hypertrophy that is associated with an increase in basal intracellular Na<sup>+</sup> and Ca<sup>2+</sup> levels. Furthermore, long-term taurine treatment prevents morphological and ionic remodeling induced by insulin. In addition, blocking the Na<sup>+</sup>-taurine co-transporter prevented the taurine antihypertrophic effect. Finally, the insulin-induced remodeling of cardiomyocytes was associated with a decrease in the ratio of phospho-CREB (pCREB) to total cAMP response element binding protein (CREB); taurine prevented this effect. In conclusion, our results show that insulin induces ventricular cardiomyocyte hypertrophy via downregulation of the pCREB/tCREB level and that chronic taurine treatment prevents this effect.https://www.mdpi.com/2072-6643/13/11/3686taurineinsulincardiomyocyteshypertrophysodiumcalcium |
| spellingShingle | Ashley Jazzar Danielle Jacques Ghassan Bkaily Insulin-Induced Cardiomyocytes Hypertrophy That Is Prevented by Taurine via β-alanine-Sensitive Na<sup>+</sup>-Taurine Symporter Nutrients taurine insulin cardiomyocytes hypertrophy sodium calcium |
| title | Insulin-Induced Cardiomyocytes Hypertrophy That Is Prevented by Taurine via β-alanine-Sensitive Na<sup>+</sup>-Taurine Symporter |
| title_full | Insulin-Induced Cardiomyocytes Hypertrophy That Is Prevented by Taurine via β-alanine-Sensitive Na<sup>+</sup>-Taurine Symporter |
| title_fullStr | Insulin-Induced Cardiomyocytes Hypertrophy That Is Prevented by Taurine via β-alanine-Sensitive Na<sup>+</sup>-Taurine Symporter |
| title_full_unstemmed | Insulin-Induced Cardiomyocytes Hypertrophy That Is Prevented by Taurine via β-alanine-Sensitive Na<sup>+</sup>-Taurine Symporter |
| title_short | Insulin-Induced Cardiomyocytes Hypertrophy That Is Prevented by Taurine via β-alanine-Sensitive Na<sup>+</sup>-Taurine Symporter |
| title_sort | insulin induced cardiomyocytes hypertrophy that is prevented by taurine via β alanine sensitive na sup sup taurine symporter |
| topic | taurine insulin cardiomyocytes hypertrophy sodium calcium |
| url | https://www.mdpi.com/2072-6643/13/11/3686 |
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