HETEROGENEITY OF POLYCLONAL IMMUNOGLOBULINS NUCLEASE ACTIVITY IN RHEUMATOID AND REACTIVE ARTHRITIS
Catalytic properties of immunoglobulins are widely studied within recent years. It was found that nuclease activity of immunoglobulins is increased in systemic autoimmune diseases. Given some pathogenetic features of rheumatoid arthritis and reactive arthritis, it is appropriate to clarify the natur...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2017-12-01
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| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/1401 |
| _version_ | 1797197931289247744 |
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| author | M. V. Volkova A. V. Kunder I. I. Generalov D. M. Petrovich |
| author_facet | M. V. Volkova A. V. Kunder I. I. Generalov D. M. Petrovich |
| author_sort | M. V. Volkova |
| collection | DOAJ |
| description | Catalytic properties of immunoglobulins are widely studied within recent years. It was found that nuclease activity of immunoglobulins is increased in systemic autoimmune diseases. Given some pathogenetic features of rheumatoid arthritis and reactive arthritis, it is appropriate to clarify the nature of nuclease activity in these diseases. Determination of DNAse activity of immunoglobulins with different DNA substrates, and search for specific substrates for distinct clinical entities could serve these purposes. The aim of present work is to determine DNase activity of the polyclonal class G immunoglobulins in rheumatoid and reactive arthritis using various methods.Different methods are used to evaluate nuclease activity. In this paper we present newly developed and modified techniques for determination of DNAse activity of polyclonal IgGs. Particular attention was paid to the electrophoretic method of DNase activity assessment. Polyclonal IgG isolated from blood serum of patients with rheumatoid arthritis and reactive arthritis were used for assays. In this study, we demonstrated the presence of an inhomogeneous DNase activity of immunoglobulins in relation to different substrates.Along with calf thymus DNA, we used bacterial plasmid DNA and PCR products based on bacterial gene sequences. Levels of DNase activity by rivanol clot method with calf thymus DNA as substrate proved to be higher in patients with rheumatoid arthritis than the control values (p < 0.01). DNase abzyme activity in patients with rheumatoid arthritis was elevated, as compared to the patients with reactive arthritis (p < 0.01).When examining ability of the IgG to hydrolyze procaryotic DNA (bacterial plasmid DNA and PCR products, based on bacterial genes), we obtained heterogeneous results. Different Ig samples showed varying degrees of DNA hydrolysis. Abzyme hydrolysis of DNA substrates longer than 700 bp was more pronounced, as compared to short DNA substrates (100 base pairs).Conclusions: 1) antibodies having deoxyribonucleic activity are present in serum of patients with rheumatoid arthritis, reactive arthritis; 2)DNase abzymes exhibit heterogeneous ability to hydrolyze various DNA substrates; 3) Abzyme-medicted hydrolysis of DNA substrates longer than 700 base pairs is more pronounced, as compared to a short DNA substrates (100 bp) length. |
| first_indexed | 2024-03-08T05:48:27Z |
| format | Article |
| id | doaj.art-23c86a4a3c1f49a298d392ffdc493365 |
| institution | Directory Open Access Journal |
| issn | 1563-0625 2313-741X |
| language | Russian |
| last_indexed | 2024-04-24T06:51:48Z |
| publishDate | 2017-12-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj.art-23c86a4a3c1f49a298d392ffdc4933652024-04-22T13:07:42ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2017-12-0119677177810.15789/1563-0625-2017-6-771-778972HETEROGENEITY OF POLYCLONAL IMMUNOGLOBULINS NUCLEASE ACTIVITY IN RHEUMATOID AND REACTIVE ARTHRITISM. V. Volkova0A. V. Kunder1I. I. Generalov2D. M. Petrovich3Belorussian Medical Academy of Postgraduate EducationBelorussian Medical Academy of Postgraduate EducationVitebsk State Medical UniversityBelorussian Medical Academy of Postgraduate EducationCatalytic properties of immunoglobulins are widely studied within recent years. It was found that nuclease activity of immunoglobulins is increased in systemic autoimmune diseases. Given some pathogenetic features of rheumatoid arthritis and reactive arthritis, it is appropriate to clarify the nature of nuclease activity in these diseases. Determination of DNAse activity of immunoglobulins with different DNA substrates, and search for specific substrates for distinct clinical entities could serve these purposes. The aim of present work is to determine DNase activity of the polyclonal class G immunoglobulins in rheumatoid and reactive arthritis using various methods.Different methods are used to evaluate nuclease activity. In this paper we present newly developed and modified techniques for determination of DNAse activity of polyclonal IgGs. Particular attention was paid to the electrophoretic method of DNase activity assessment. Polyclonal IgG isolated from blood serum of patients with rheumatoid arthritis and reactive arthritis were used for assays. In this study, we demonstrated the presence of an inhomogeneous DNase activity of immunoglobulins in relation to different substrates.Along with calf thymus DNA, we used bacterial plasmid DNA and PCR products based on bacterial gene sequences. Levels of DNase activity by rivanol clot method with calf thymus DNA as substrate proved to be higher in patients with rheumatoid arthritis than the control values (p < 0.01). DNase abzyme activity in patients with rheumatoid arthritis was elevated, as compared to the patients with reactive arthritis (p < 0.01).When examining ability of the IgG to hydrolyze procaryotic DNA (bacterial plasmid DNA and PCR products, based on bacterial genes), we obtained heterogeneous results. Different Ig samples showed varying degrees of DNA hydrolysis. Abzyme hydrolysis of DNA substrates longer than 700 bp was more pronounced, as compared to short DNA substrates (100 base pairs).Conclusions: 1) antibodies having deoxyribonucleic activity are present in serum of patients with rheumatoid arthritis, reactive arthritis; 2)DNase abzymes exhibit heterogeneous ability to hydrolyze various DNA substrates; 3) Abzyme-medicted hydrolysis of DNA substrates longer than 700 base pairs is more pronounced, as compared to a short DNA substrates (100 bp) length.https://www.mimmun.ru/mimmun/article/view/1401immunoglobulinsabzymesnuclease activityheterogeneityrheumatoid arthritisreactive arthritis |
| spellingShingle | M. V. Volkova A. V. Kunder I. I. Generalov D. M. Petrovich HETEROGENEITY OF POLYCLONAL IMMUNOGLOBULINS NUCLEASE ACTIVITY IN RHEUMATOID AND REACTIVE ARTHRITIS Медицинская иммунология immunoglobulins abzymes nuclease activity heterogeneity rheumatoid arthritis reactive arthritis |
| title | HETEROGENEITY OF POLYCLONAL IMMUNOGLOBULINS NUCLEASE ACTIVITY IN RHEUMATOID AND REACTIVE ARTHRITIS |
| title_full | HETEROGENEITY OF POLYCLONAL IMMUNOGLOBULINS NUCLEASE ACTIVITY IN RHEUMATOID AND REACTIVE ARTHRITIS |
| title_fullStr | HETEROGENEITY OF POLYCLONAL IMMUNOGLOBULINS NUCLEASE ACTIVITY IN RHEUMATOID AND REACTIVE ARTHRITIS |
| title_full_unstemmed | HETEROGENEITY OF POLYCLONAL IMMUNOGLOBULINS NUCLEASE ACTIVITY IN RHEUMATOID AND REACTIVE ARTHRITIS |
| title_short | HETEROGENEITY OF POLYCLONAL IMMUNOGLOBULINS NUCLEASE ACTIVITY IN RHEUMATOID AND REACTIVE ARTHRITIS |
| title_sort | heterogeneity of polyclonal immunoglobulins nuclease activity in rheumatoid and reactive arthritis |
| topic | immunoglobulins abzymes nuclease activity heterogeneity rheumatoid arthritis reactive arthritis |
| url | https://www.mimmun.ru/mimmun/article/view/1401 |
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