Comparative transcriptome analysis of roots, stems and leaves of Isodon amethystoides reveals candidate genes involved in Wangzaozins biosynthesis
Abstract Background Isodon amethystoides (Ben-th) Cy Wu et Hsuan is an important traditional medicinal plant endowed with pharmacological properties effective in the treatment of various diseases, including pulmonary tuberculosis. The tetracyclic diterpenoids, Wangzaozins (Wangzaozin A, glaucocalyxi...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-11-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12870-018-1505-0 |
| _version_ | 1818138238189043712 |
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| author | Fenglan Zhao Mengchu Sun Wanjun Zhang Chunli Jiang Jingtong Teng Wei Sheng Mingzhi Li Aimin Zhang Yongbo Duan Jianping Xue |
| author_facet | Fenglan Zhao Mengchu Sun Wanjun Zhang Chunli Jiang Jingtong Teng Wei Sheng Mingzhi Li Aimin Zhang Yongbo Duan Jianping Xue |
| author_sort | Fenglan Zhao |
| collection | DOAJ |
| description | Abstract Background Isodon amethystoides (Ben-th) Cy Wu et Hsuan is an important traditional medicinal plant endowed with pharmacological properties effective in the treatment of various diseases, including pulmonary tuberculosis. The tetracyclic diterpenoids, Wangzaozins (Wangzaozin A, glaucocalyxin A, glaucocalyxin B), are the major bioactive compounds of I. amethystoides. However, the molecular information about the biosynthesis of these compounds still remains unclear. Results An examination of the accumulated levels of Wangzaozins in I. amethystoides revealed considerable variations in the root, stem, and leaf tissues of this plant, indicating possible differences in metabolite biosynthesis and accumulation among various tissues. To better elucidate the tetracyclic diterpenoid biosynthesis pathway, we generated transcriptome sequences from the root, stem, and leaf tissues, and performed de novo sequence assembly, yielding 230,974 transcripts and 114,488 unigenes, with average N50 lengths of 1914 and 1241 bp, respectively. Putative functions could be assigned to 73,693 transcripts (31.9%) based on BLAST searches against annotation databases, including GO, KEGG, Swiss-Prot, NR, and Pfam. Moreover, the candidate genes involving in the diterpenoid biosynthesis, such as CPS, KSL, were also analyzed. The expression profiles of eight transcripts, involving the tetracyclic diterpenoid biosynthesis, were validated in different I. amethystoides tissues by qRT-PCR, unraveling the gene expression profile of the pathway. The differential expressions of ISPD, ISPF and ISPH (MEP pathway), and IaCPS and IaKSL (diterpenoid pathway) candidate genes in leaves and roots, may contribute to the high accumulation of Wangzaozins in I. amethystoides leaves. Conclusion The genomic dataset and analyses reported here lay the foundations for further research on this important medicinal plant. |
| first_indexed | 2024-12-11T10:09:01Z |
| format | Article |
| id | doaj.art-23c916b80e994d4fa913755e2fd5483f |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-11T10:09:01Z |
| publishDate | 2018-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-23c916b80e994d4fa913755e2fd5483f2022-12-22T01:11:48ZengBMCBMC Plant Biology1471-22292018-11-0118111310.1186/s12870-018-1505-0Comparative transcriptome analysis of roots, stems and leaves of Isodon amethystoides reveals candidate genes involved in Wangzaozins biosynthesisFenglan Zhao0Mengchu Sun1Wanjun Zhang2Chunli Jiang3Jingtong Teng4Wei Sheng5Mingzhi Li6Aimin Zhang7Yongbo Duan8Jianping Xue9Key Laboratory of Resource Plant Biology of Anhui Province, College of Life Sciences, Huaibei Normal UniversityKey Laboratory of Resource Plant Biology of Anhui Province, College of Life Sciences, Huaibei Normal UniversityKey Laboratory of Resource Plant Biology of Anhui Province, College of Life Sciences, Huaibei Normal UniversityKey Laboratory of Resource Plant Biology of Anhui Province, College of Life Sciences, Huaibei Normal UniversityKey Laboratory of Resource Plant Biology of Anhui Province, College of Life Sciences, Huaibei Normal UniversityKey Laboratory of Resource Plant Biology of Anhui Province, College of Life Sciences, Huaibei Normal UniversityGenepioneer Biotechnologies Co. LtdKey Laboratory of Resource Plant Biology of Anhui Province, College of Life Sciences, Huaibei Normal UniversityKey Laboratory of Resource Plant Biology of Anhui Province, College of Life Sciences, Huaibei Normal UniversityKey Laboratory of Resource Plant Biology of Anhui Province, College of Life Sciences, Huaibei Normal UniversityAbstract Background Isodon amethystoides (Ben-th) Cy Wu et Hsuan is an important traditional medicinal plant endowed with pharmacological properties effective in the treatment of various diseases, including pulmonary tuberculosis. The tetracyclic diterpenoids, Wangzaozins (Wangzaozin A, glaucocalyxin A, glaucocalyxin B), are the major bioactive compounds of I. amethystoides. However, the molecular information about the biosynthesis of these compounds still remains unclear. Results An examination of the accumulated levels of Wangzaozins in I. amethystoides revealed considerable variations in the root, stem, and leaf tissues of this plant, indicating possible differences in metabolite biosynthesis and accumulation among various tissues. To better elucidate the tetracyclic diterpenoid biosynthesis pathway, we generated transcriptome sequences from the root, stem, and leaf tissues, and performed de novo sequence assembly, yielding 230,974 transcripts and 114,488 unigenes, with average N50 lengths of 1914 and 1241 bp, respectively. Putative functions could be assigned to 73,693 transcripts (31.9%) based on BLAST searches against annotation databases, including GO, KEGG, Swiss-Prot, NR, and Pfam. Moreover, the candidate genes involving in the diterpenoid biosynthesis, such as CPS, KSL, were also analyzed. The expression profiles of eight transcripts, involving the tetracyclic diterpenoid biosynthesis, were validated in different I. amethystoides tissues by qRT-PCR, unraveling the gene expression profile of the pathway. The differential expressions of ISPD, ISPF and ISPH (MEP pathway), and IaCPS and IaKSL (diterpenoid pathway) candidate genes in leaves and roots, may contribute to the high accumulation of Wangzaozins in I. amethystoides leaves. Conclusion The genomic dataset and analyses reported here lay the foundations for further research on this important medicinal plant.http://link.springer.com/article/10.1186/s12870-018-1505-0Isodon amethystoides (ben-th) cy Wu et HsuanTranscriptomede novo assemblyTetracyclic diterpenoidWangzaozins |
| spellingShingle | Fenglan Zhao Mengchu Sun Wanjun Zhang Chunli Jiang Jingtong Teng Wei Sheng Mingzhi Li Aimin Zhang Yongbo Duan Jianping Xue Comparative transcriptome analysis of roots, stems and leaves of Isodon amethystoides reveals candidate genes involved in Wangzaozins biosynthesis BMC Plant Biology Isodon amethystoides (ben-th) cy Wu et Hsuan Transcriptome de novo assembly Tetracyclic diterpenoid Wangzaozins |
| title | Comparative transcriptome analysis of roots, stems and leaves of Isodon amethystoides reveals candidate genes involved in Wangzaozins biosynthesis |
| title_full | Comparative transcriptome analysis of roots, stems and leaves of Isodon amethystoides reveals candidate genes involved in Wangzaozins biosynthesis |
| title_fullStr | Comparative transcriptome analysis of roots, stems and leaves of Isodon amethystoides reveals candidate genes involved in Wangzaozins biosynthesis |
| title_full_unstemmed | Comparative transcriptome analysis of roots, stems and leaves of Isodon amethystoides reveals candidate genes involved in Wangzaozins biosynthesis |
| title_short | Comparative transcriptome analysis of roots, stems and leaves of Isodon amethystoides reveals candidate genes involved in Wangzaozins biosynthesis |
| title_sort | comparative transcriptome analysis of roots stems and leaves of isodon amethystoides reveals candidate genes involved in wangzaozins biosynthesis |
| topic | Isodon amethystoides (ben-th) cy Wu et Hsuan Transcriptome de novo assembly Tetracyclic diterpenoid Wangzaozins |
| url | http://link.springer.com/article/10.1186/s12870-018-1505-0 |
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