Selection and characterization of extracellular enzyme production by an endophytic fungi Aspergillus sojae and its bio-efficacy analysis against cotton leaf worm, Spodoptera litura
This study was intended to isolate and investigate endophytic fungi and its metabolite as an active biocontrol agent. Endophytic fungal secondary metabolites are considered as an effective and environmentally friendly alternative to synthetic chemicals for integrated pest management. In this study,...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-09-01
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| Series: | Current Plant Biology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214662820300347 |
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| author | Duraisamy Elango Velu Manikandan Palaniyappan Jayanthi Palanivel Velmurugan Balasubramanian Balamuralikrishnan Arumugam Veera Ravi Muthugounder Subramaniam Shivakumar |
| author_facet | Duraisamy Elango Velu Manikandan Palaniyappan Jayanthi Palanivel Velmurugan Balasubramanian Balamuralikrishnan Arumugam Veera Ravi Muthugounder Subramaniam Shivakumar |
| author_sort | Duraisamy Elango |
| collection | DOAJ |
| description | This study was intended to isolate and investigate endophytic fungi and its metabolite as an active biocontrol agent. Endophytic fungal secondary metabolites are considered as an effective and environmentally friendly alternative to synthetic chemicals for integrated pest management. In this study, different parts of medicinal plant Plectranthus amboinicus (Oregano) were selected to isolate twenty-three fungal endophytes. Out of twenty-three, one isolate demonstrates potent activities against cotton leaf worm Spodoptera litura (S. litura) an insecticides resistance pest. Identification of potent isolate was attained through the morphological, sequence similarity and phylogenetic analysis of the ITS regions and confirmed as Aspergillus sojae (A. sojae). The GCMS analysis reveals the metabolites of A. sojae were rich in 2-Furancarboxaldehyde and Levoglucosenone then other compounds. The FTIR peaks of A. sojae crude extract shows the major peaks that corresponding to NH, C–H, and OH stretching and minor peaks are C–H bend (meta) and acetylenic C–H bend. FTIR analysis supports the presence of functional groups in accordance with GCMS data as dominating compound. The bioassay results showed that A. sojae metabolite affects third and fourth instar larvae of S. litura. In addition, antibiotic quality of A. sojae metabolite tested against five selective bacteria. Among five different bacteria, the maximum zone of inhibition was displayed for Escherichia coli and Staphylococcus aureus in the higher concentration of 100 μg/mL. Our resultssuggest the importance of endophytic fungal metabolites to control the pesticide-resistant insect pests. |
| first_indexed | 2024-12-22T16:58:32Z |
| format | Article |
| id | doaj.art-23cbbec1984b489bb37b9344f850575b |
| institution | Directory Open Access Journal |
| issn | 2214-6628 |
| language | English |
| last_indexed | 2024-12-22T16:58:32Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Plant Biology |
| spelling | doaj.art-23cbbec1984b489bb37b9344f850575b2022-12-21T18:19:22ZengElsevierCurrent Plant Biology2214-66282020-09-0123100153Selection and characterization of extracellular enzyme production by an endophytic fungi Aspergillus sojae and its bio-efficacy analysis against cotton leaf worm, Spodoptera lituraDuraisamy Elango0Velu Manikandan1Palaniyappan Jayanthi2Palanivel Velmurugan3Balasubramanian Balamuralikrishnan4Arumugam Veera Ravi5Muthugounder Subramaniam Shivakumar6Molecular Entomology Lab, Department of Biotechnology, Periyar University, Salem, 636011, Tamil Nadu, India; Department of Environmental Science, Periyar University, Salem, 636011, Tamil Nadu, IndiaDepartment of Environmental Science, Periyar University, Salem, 636011, Tamil Nadu, IndiaDepartment of Environmental Science, Periyar University, Salem, 636011, Tamil Nadu, IndiaDepartment of Biotechnology, Science Campus, Alagappa University, Karaikudi, 630003, Tamil Nadu, IndiaDepartment of Food Science and Biotechnology, College of Life Science, Sejong University, Seoul 05006, South KoreaDepartment of Biotechnology, Science Campus, Alagappa University, Karaikudi, 630003, Tamil Nadu, IndiaMolecular Entomology Lab, Department of Biotechnology, Periyar University, Salem, 636011, Tamil Nadu, India; Corresponding author.This study was intended to isolate and investigate endophytic fungi and its metabolite as an active biocontrol agent. Endophytic fungal secondary metabolites are considered as an effective and environmentally friendly alternative to synthetic chemicals for integrated pest management. In this study, different parts of medicinal plant Plectranthus amboinicus (Oregano) were selected to isolate twenty-three fungal endophytes. Out of twenty-three, one isolate demonstrates potent activities against cotton leaf worm Spodoptera litura (S. litura) an insecticides resistance pest. Identification of potent isolate was attained through the morphological, sequence similarity and phylogenetic analysis of the ITS regions and confirmed as Aspergillus sojae (A. sojae). The GCMS analysis reveals the metabolites of A. sojae were rich in 2-Furancarboxaldehyde and Levoglucosenone then other compounds. The FTIR peaks of A. sojae crude extract shows the major peaks that corresponding to NH, C–H, and OH stretching and minor peaks are C–H bend (meta) and acetylenic C–H bend. FTIR analysis supports the presence of functional groups in accordance with GCMS data as dominating compound. The bioassay results showed that A. sojae metabolite affects third and fourth instar larvae of S. litura. In addition, antibiotic quality of A. sojae metabolite tested against five selective bacteria. Among five different bacteria, the maximum zone of inhibition was displayed for Escherichia coli and Staphylococcus aureus in the higher concentration of 100 μg/mL. Our resultssuggest the importance of endophytic fungal metabolites to control the pesticide-resistant insect pests.http://www.sciencedirect.com/science/article/pii/S2214662820300347Endophytic fungiAspergillus sojaeExtracellular enzymeGC–MSFTIRSpodoptera litura |
| spellingShingle | Duraisamy Elango Velu Manikandan Palaniyappan Jayanthi Palanivel Velmurugan Balasubramanian Balamuralikrishnan Arumugam Veera Ravi Muthugounder Subramaniam Shivakumar Selection and characterization of extracellular enzyme production by an endophytic fungi Aspergillus sojae and its bio-efficacy analysis against cotton leaf worm, Spodoptera litura Current Plant Biology Endophytic fungi Aspergillus sojae Extracellular enzyme GC–MS FTIR Spodoptera litura |
| title | Selection and characterization of extracellular enzyme production by an endophytic fungi Aspergillus sojae and its bio-efficacy analysis against cotton leaf worm, Spodoptera litura |
| title_full | Selection and characterization of extracellular enzyme production by an endophytic fungi Aspergillus sojae and its bio-efficacy analysis against cotton leaf worm, Spodoptera litura |
| title_fullStr | Selection and characterization of extracellular enzyme production by an endophytic fungi Aspergillus sojae and its bio-efficacy analysis against cotton leaf worm, Spodoptera litura |
| title_full_unstemmed | Selection and characterization of extracellular enzyme production by an endophytic fungi Aspergillus sojae and its bio-efficacy analysis against cotton leaf worm, Spodoptera litura |
| title_short | Selection and characterization of extracellular enzyme production by an endophytic fungi Aspergillus sojae and its bio-efficacy analysis against cotton leaf worm, Spodoptera litura |
| title_sort | selection and characterization of extracellular enzyme production by an endophytic fungi aspergillus sojae and its bio efficacy analysis against cotton leaf worm spodoptera litura |
| topic | Endophytic fungi Aspergillus sojae Extracellular enzyme GC–MS FTIR Spodoptera litura |
| url | http://www.sciencedirect.com/science/article/pii/S2214662820300347 |
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