Diagnostic system for the detection of severe fever with thrombocytopenia syndrome virus RNA from suspected infected animals.
<h4>Background</h4>Severe fever with thrombocytopenia syndrome virus (SFTSV) causes severe hemorrhagic fever in humans and cats. Clinical symptoms of SFTS-infected cats resemble those of SFTS patients, whereas SFTS-contracted cats have high levels of viral RNA loads in the serum and body...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0238671 |
| _version_ | 1819032190469537792 |
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| author | Eun-Sil Park Osamu Fujita Masanobu Kimura Akitoyo Hotta Koichi Imaoka Masayuki Shimojima Masayuki Saijo Ken Maeda Shigeru Morikawa |
| author_facet | Eun-Sil Park Osamu Fujita Masanobu Kimura Akitoyo Hotta Koichi Imaoka Masayuki Shimojima Masayuki Saijo Ken Maeda Shigeru Morikawa |
| author_sort | Eun-Sil Park |
| collection | DOAJ |
| description | <h4>Background</h4>Severe fever with thrombocytopenia syndrome virus (SFTSV) causes severe hemorrhagic fever in humans and cats. Clinical symptoms of SFTS-infected cats resemble those of SFTS patients, whereas SFTS-contracted cats have high levels of viral RNA loads in the serum and body fluids. Due to the risk of direct infection from SFTS-infected cats to human, it is important to diagnose SFTS-suspected animals. In this study, a reverse transcription polymerase chain reaction (RT-PCR) was newly developed to diagnose SFTS-suspected animals without non-specific reactions.<h4>Methodology/principle findings</h4>Four primer sets were newly designed from consensus sequences constructed from 108 strains of SFTSV. A RT-PCR with these four primer sets successfully and specifically detected four clades of SFTSV. Their limits of detection are 1-10 copies/reaction. Using this RT-PCR, 5 cat cases among 56 SFTS-suspected animal cases were diagnosed as SFTS. From these cats, IgM or IgG against SFTSV were detected by enzyme-linked immunosorbent assay (ELISA), but not neutralizing antibodies by plaque reduction neutralization titer (PRNT) test. This phenomenon is similar to those of fatal SFTS patients.<h4>Conclusion/significance</h4>This newly developed RT-PCR could detect SFTSV RNA of several clades and from SFTS-suspected animals. In addition to ELISA and PRNT test, the useful laboratory diagnosis systems of SFTS-suspected animals has been made in this study. |
| first_indexed | 2024-12-21T06:58:00Z |
| format | Article |
| id | doaj.art-23ce3964372d4f27973499d52767bade |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T06:58:00Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-23ce3964372d4f27973499d52767bade2022-12-21T19:12:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01161e023867110.1371/journal.pone.0238671Diagnostic system for the detection of severe fever with thrombocytopenia syndrome virus RNA from suspected infected animals.Eun-Sil ParkOsamu FujitaMasanobu KimuraAkitoyo HottaKoichi ImaokaMasayuki ShimojimaMasayuki SaijoKen MaedaShigeru Morikawa<h4>Background</h4>Severe fever with thrombocytopenia syndrome virus (SFTSV) causes severe hemorrhagic fever in humans and cats. Clinical symptoms of SFTS-infected cats resemble those of SFTS patients, whereas SFTS-contracted cats have high levels of viral RNA loads in the serum and body fluids. Due to the risk of direct infection from SFTS-infected cats to human, it is important to diagnose SFTS-suspected animals. In this study, a reverse transcription polymerase chain reaction (RT-PCR) was newly developed to diagnose SFTS-suspected animals without non-specific reactions.<h4>Methodology/principle findings</h4>Four primer sets were newly designed from consensus sequences constructed from 108 strains of SFTSV. A RT-PCR with these four primer sets successfully and specifically detected four clades of SFTSV. Their limits of detection are 1-10 copies/reaction. Using this RT-PCR, 5 cat cases among 56 SFTS-suspected animal cases were diagnosed as SFTS. From these cats, IgM or IgG against SFTSV were detected by enzyme-linked immunosorbent assay (ELISA), but not neutralizing antibodies by plaque reduction neutralization titer (PRNT) test. This phenomenon is similar to those of fatal SFTS patients.<h4>Conclusion/significance</h4>This newly developed RT-PCR could detect SFTSV RNA of several clades and from SFTS-suspected animals. In addition to ELISA and PRNT test, the useful laboratory diagnosis systems of SFTS-suspected animals has been made in this study.https://doi.org/10.1371/journal.pone.0238671 |
| spellingShingle | Eun-Sil Park Osamu Fujita Masanobu Kimura Akitoyo Hotta Koichi Imaoka Masayuki Shimojima Masayuki Saijo Ken Maeda Shigeru Morikawa Diagnostic system for the detection of severe fever with thrombocytopenia syndrome virus RNA from suspected infected animals. PLoS ONE |
| title | Diagnostic system for the detection of severe fever with thrombocytopenia syndrome virus RNA from suspected infected animals. |
| title_full | Diagnostic system for the detection of severe fever with thrombocytopenia syndrome virus RNA from suspected infected animals. |
| title_fullStr | Diagnostic system for the detection of severe fever with thrombocytopenia syndrome virus RNA from suspected infected animals. |
| title_full_unstemmed | Diagnostic system for the detection of severe fever with thrombocytopenia syndrome virus RNA from suspected infected animals. |
| title_short | Diagnostic system for the detection of severe fever with thrombocytopenia syndrome virus RNA from suspected infected animals. |
| title_sort | diagnostic system for the detection of severe fever with thrombocytopenia syndrome virus rna from suspected infected animals |
| url | https://doi.org/10.1371/journal.pone.0238671 |
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