Label-Free, Impedance-Based Biosensor for Kidney Disease Biomarker Uromodulin
We demonstrate the development of a label-free, impedance-based biosensor by using a passivation layer of 50-nm tantalum pentoxide (Ta<sub>2</sub>O<sub>5</sub>) on interdigitated electrodes (IDE). This layer was fabricated by atomic layer deposition (ALD) and has a high diele...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/23/24/9696 |
| _version_ | 1797379407672770560 |
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| author | Kunj Vora Norbert Kordas Karsten Seidl |
| author_facet | Kunj Vora Norbert Kordas Karsten Seidl |
| author_sort | Kunj Vora |
| collection | DOAJ |
| description | We demonstrate the development of a label-free, impedance-based biosensor by using a passivation layer of 50-nm tantalum pentoxide (Ta<sub>2</sub>O<sub>5</sub>) on interdigitated electrodes (IDE). This layer was fabricated by atomic layer deposition (ALD) and has a high dielectric constant (high-<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>κ</mi></semantics></math></inline-formula>), which improves the capacitive property of the IDE. We validate the biosensor’s performance by measuring uromodulin, a urine biomarker for kidney tubular damage, from artificial urine samples. The passivation layer is functionalized with uromodulin antibodies for selective binding. The passivated IDE enables the non-faradaic impedance measurement of uromodulin concentrations with a measurement range from 0.5 ng/mL to 8 ng/mL and with a relative change in impedance of 15 % per ng/mL at a frequency of 150 Hz (log scale). This work presents a concept for point-of-care biosensing applications for disease biomarkers. |
| first_indexed | 2024-03-08T20:22:47Z |
| format | Article |
| id | doaj.art-23cf17ad67004c93ac5b9474a6af728d |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-08T20:22:47Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-23cf17ad67004c93ac5b9474a6af728d2023-12-22T14:40:16ZengMDPI AGSensors1424-82202023-12-012324969610.3390/s23249696Label-Free, Impedance-Based Biosensor for Kidney Disease Biomarker UromodulinKunj Vora0Norbert Kordas1Karsten Seidl2Fraunhofer Institute for Microelectronic Circuits and Systems, 47057 Duisburg, GermanyFraunhofer Institute for Microelectronic Circuits and Systems, 47057 Duisburg, GermanyFraunhofer Institute for Microelectronic Circuits and Systems, 47057 Duisburg, GermanyWe demonstrate the development of a label-free, impedance-based biosensor by using a passivation layer of 50-nm tantalum pentoxide (Ta<sub>2</sub>O<sub>5</sub>) on interdigitated electrodes (IDE). This layer was fabricated by atomic layer deposition (ALD) and has a high dielectric constant (high-<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>κ</mi></semantics></math></inline-formula>), which improves the capacitive property of the IDE. We validate the biosensor’s performance by measuring uromodulin, a urine biomarker for kidney tubular damage, from artificial urine samples. The passivation layer is functionalized with uromodulin antibodies for selective binding. The passivated IDE enables the non-faradaic impedance measurement of uromodulin concentrations with a measurement range from 0.5 ng/mL to 8 ng/mL and with a relative change in impedance of 15 % per ng/mL at a frequency of 150 Hz (log scale). This work presents a concept for point-of-care biosensing applications for disease biomarkers.https://www.mdpi.com/1424-8220/23/24/9696impedance spectroscopyatomic layer depositiontantalum pentoxideuromodulinchronic kidney disease |
| spellingShingle | Kunj Vora Norbert Kordas Karsten Seidl Label-Free, Impedance-Based Biosensor for Kidney Disease Biomarker Uromodulin Sensors impedance spectroscopy atomic layer deposition tantalum pentoxide uromodulin chronic kidney disease |
| title | Label-Free, Impedance-Based Biosensor for Kidney Disease Biomarker Uromodulin |
| title_full | Label-Free, Impedance-Based Biosensor for Kidney Disease Biomarker Uromodulin |
| title_fullStr | Label-Free, Impedance-Based Biosensor for Kidney Disease Biomarker Uromodulin |
| title_full_unstemmed | Label-Free, Impedance-Based Biosensor for Kidney Disease Biomarker Uromodulin |
| title_short | Label-Free, Impedance-Based Biosensor for Kidney Disease Biomarker Uromodulin |
| title_sort | label free impedance based biosensor for kidney disease biomarker uromodulin |
| topic | impedance spectroscopy atomic layer deposition tantalum pentoxide uromodulin chronic kidney disease |
| url | https://www.mdpi.com/1424-8220/23/24/9696 |
| work_keys_str_mv | AT kunjvora labelfreeimpedancebasedbiosensorforkidneydiseasebiomarkeruromodulin AT norbertkordas labelfreeimpedancebasedbiosensorforkidneydiseasebiomarkeruromodulin AT karstenseidl labelfreeimpedancebasedbiosensorforkidneydiseasebiomarkeruromodulin |