Biohybrid elastin-like venous valve with potential for in situ tissue engineering
Chronic venous insufficiency (CVI) is a leading vascular disease whose clinical manifestations include varicose veins, edemas, venous ulcers, and venous hypertension, among others. Therapies targeting this medical issue are scarce, and so far, no single venous valve prosthesis is clinically availabl...
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| Format: | Article |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.988533/full |
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| author | Fernando González-Pérez Sergio Acosta Stephan Rütten Caroline Emonts Alexander Kopp Heinz-Werner Henke Philipp Bruners Thomas Gries J. Carlos Rodríguez-Cabello Stefan Jockenhoevel Stefan Jockenhoevel Alicia Fernández-Colino |
| author_facet | Fernando González-Pérez Sergio Acosta Stephan Rütten Caroline Emonts Alexander Kopp Heinz-Werner Henke Philipp Bruners Thomas Gries J. Carlos Rodríguez-Cabello Stefan Jockenhoevel Stefan Jockenhoevel Alicia Fernández-Colino |
| author_sort | Fernando González-Pérez |
| collection | DOAJ |
| description | Chronic venous insufficiency (CVI) is a leading vascular disease whose clinical manifestations include varicose veins, edemas, venous ulcers, and venous hypertension, among others. Therapies targeting this medical issue are scarce, and so far, no single venous valve prosthesis is clinically available. Herein, we have designed a bi-leaflet transcatheter venous valve that consists of (i) elastin-like recombinamers, (ii) a textile mesh reinforcement, and (iii) a bioabsorbable magnesium stent structure. Mechanical characterization of the resulting biohybrid elastin-like venous valves (EVV) showed an anisotropic behavior equivalent to the native bovine saphenous vein valves and mechanical strength suitable for vascular implantation. The EVV also featured minimal hemolysis and platelet adhesion, besides actively supporting endothelialization in vitro, thus setting the basis for its application as an in situ tissue engineering implant. In addition, the hydrodynamic testing in a pulsatile bioreactor demonstrated excellent hemodynamic valve performance, with minimal regurgitation (<10%) and pressure drop (<5 mmHg). No stagnation points were detected and an in vitro simulated transcatheter delivery showed the ability of the venous valve to withstand the implantation procedure. These results present a promising concept of a biohybrid transcatheter venous valve as an off-the-shelf implant, with great potential to provide clinical solutions for CVI treatment. |
| first_indexed | 2024-12-10T14:05:12Z |
| format | Article |
| id | doaj.art-23d12ac74a724346bbac120d173aa8ba |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-10T14:05:12Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-23d12ac74a724346bbac120d173aa8ba2022-12-22T01:45:40ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-09-011010.3389/fbioe.2022.988533988533Biohybrid elastin-like venous valve with potential for in situ tissue engineeringFernando González-Pérez0Sergio Acosta1Stephan Rütten2Caroline Emonts3Alexander Kopp4Heinz-Werner Henke5Philipp Bruners6Thomas Gries7J. Carlos Rodríguez-Cabello8Stefan Jockenhoevel9Stefan Jockenhoevel10Alicia Fernández-Colino11Bioforge Lab (Group for Advanced Materials and Nanobiotechnology), CIBER-BBN, Edificio LUCIA, Universidad de Valladolid, Valladolid, SpainDepartment of Biohybrid and Medical Textiles (BioTex), AME–Institute of Applied Medical Engineering, Helmholtz Institute, RWTH Aachen University, Aachen, GermanyElectron Microscopy Facility, Uniklinik RWTH Aachen, Aachen, GermanyInstitut für Textiltechnik Aachen (ITA), RWTH Aachen University, Aachen, GermanyMeotec GmbH, Aachen, GermanyInnovative Tomography Products GmbH, Bochum, GermanyKlinik für Diagnostische and Interventionelle Radiologie, Universitätsklinikum Aachen, Aachen, GermanyInstitut für Textiltechnik Aachen (ITA), RWTH Aachen University, Aachen, GermanyBioforge Lab (Group for Advanced Materials and Nanobiotechnology), CIBER-BBN, Edificio LUCIA, Universidad de Valladolid, Valladolid, SpainDepartment of Biohybrid and Medical Textiles (BioTex), AME–Institute of Applied Medical Engineering, Helmholtz Institute, RWTH Aachen University, Aachen, GermanyAMIBM-Aachen-Maastricht-Institute for Biobased Materials, Maastricht University, Maastricht, NetherlandsDepartment of Biohybrid and Medical Textiles (BioTex), AME–Institute of Applied Medical Engineering, Helmholtz Institute, RWTH Aachen University, Aachen, GermanyChronic venous insufficiency (CVI) is a leading vascular disease whose clinical manifestations include varicose veins, edemas, venous ulcers, and venous hypertension, among others. Therapies targeting this medical issue are scarce, and so far, no single venous valve prosthesis is clinically available. Herein, we have designed a bi-leaflet transcatheter venous valve that consists of (i) elastin-like recombinamers, (ii) a textile mesh reinforcement, and (iii) a bioabsorbable magnesium stent structure. Mechanical characterization of the resulting biohybrid elastin-like venous valves (EVV) showed an anisotropic behavior equivalent to the native bovine saphenous vein valves and mechanical strength suitable for vascular implantation. The EVV also featured minimal hemolysis and platelet adhesion, besides actively supporting endothelialization in vitro, thus setting the basis for its application as an in situ tissue engineering implant. In addition, the hydrodynamic testing in a pulsatile bioreactor demonstrated excellent hemodynamic valve performance, with minimal regurgitation (<10%) and pressure drop (<5 mmHg). No stagnation points were detected and an in vitro simulated transcatheter delivery showed the ability of the venous valve to withstand the implantation procedure. These results present a promising concept of a biohybrid transcatheter venous valve as an off-the-shelf implant, with great potential to provide clinical solutions for CVI treatment.https://www.frontiersin.org/articles/10.3389/fbioe.2022.988533/fullminiaturized valvecell-freebioabsorbableelastin-like recombinamermagnesium stent |
| spellingShingle | Fernando González-Pérez Sergio Acosta Stephan Rütten Caroline Emonts Alexander Kopp Heinz-Werner Henke Philipp Bruners Thomas Gries J. Carlos Rodríguez-Cabello Stefan Jockenhoevel Stefan Jockenhoevel Alicia Fernández-Colino Biohybrid elastin-like venous valve with potential for in situ tissue engineering Frontiers in Bioengineering and Biotechnology miniaturized valve cell-free bioabsorbable elastin-like recombinamer magnesium stent |
| title | Biohybrid elastin-like venous valve with potential for in situ tissue engineering |
| title_full | Biohybrid elastin-like venous valve with potential for in situ tissue engineering |
| title_fullStr | Biohybrid elastin-like venous valve with potential for in situ tissue engineering |
| title_full_unstemmed | Biohybrid elastin-like venous valve with potential for in situ tissue engineering |
| title_short | Biohybrid elastin-like venous valve with potential for in situ tissue engineering |
| title_sort | biohybrid elastin like venous valve with potential for in situ tissue engineering |
| topic | miniaturized valve cell-free bioabsorbable elastin-like recombinamer magnesium stent |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.988533/full |
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