A Transcript and Metabolite Atlas of Blackcurrant Fruit Development Highlights Hormonal Regulation and Reveals the Role of Key Transcription Factors
Blackcurrant fruit collected at six stages of development were assessed for changes in gene expression using custom whole transcriptome microarrays and for variation in metabolite content using a combination of liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry. Princip...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-08-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2018.01235/full |
| _version_ | 1818693201116004352 |
|---|---|
| author | Dorota A. Jarret Jenny Morris Danny W. Cullen Sandra L. Gordon Susan R. Verrall Linda Milne Pete E. Hedley J. William Allwood Rex M. Brennan Robert D. Hancock |
| author_facet | Dorota A. Jarret Jenny Morris Danny W. Cullen Sandra L. Gordon Susan R. Verrall Linda Milne Pete E. Hedley J. William Allwood Rex M. Brennan Robert D. Hancock |
| author_sort | Dorota A. Jarret |
| collection | DOAJ |
| description | Blackcurrant fruit collected at six stages of development were assessed for changes in gene expression using custom whole transcriptome microarrays and for variation in metabolite content using a combination of liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry. Principal components analysis demonstrated that fruit development could be clearly defined according to their transcript or metabolite profiles. During early developmental stages, metabolite profiles were dominated by amino acids and tannins, whilst transcript profiles were enriched in functions associated with cell division, anatomical structure morphogenesis and cell wall metabolism. During mid fruit development, fatty acids accumulated and transcript profiles were consistent with seed and embryo development. At the later stages, sugars and anthocyanins accumulated consistent with transcript profiles that were associated with secondary metabolism. Transcript data also indicated active signaling during later stages of fruit development. A targeted analysis of signaling networks revealed a dynamic activation and repression of almost 60 different transcripts encoding transcription factors across the course of fruit development, many of which have been demonstrated as pivotal to controlling such processes in other species. Transcripts associated with cytokinin and gibberellin were highly abundant at early fruit development, whilst those associated with ABA and ethylene tended to be more abundant at later stages. The data presented here provides an insight into fruit development in blackcurrant and provides a foundation for further work in the elucidation of the genetic basis of fruit quality. |
| first_indexed | 2024-12-17T13:09:55Z |
| format | Article |
| id | doaj.art-23d1c6cac2a24921bfd014ea2674fb92 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-17T13:09:55Z |
| publishDate | 2018-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-23d1c6cac2a24921bfd014ea2674fb922022-12-21T21:47:08ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-08-01910.3389/fpls.2018.01235387510A Transcript and Metabolite Atlas of Blackcurrant Fruit Development Highlights Hormonal Regulation and Reveals the Role of Key Transcription FactorsDorota A. Jarret0Jenny Morris1Danny W. Cullen2Sandra L. Gordon3Susan R. Verrall4Linda Milne5Pete E. Hedley6J. William Allwood7Rex M. Brennan8Robert D. Hancock9James Hutton Limited, Dundee, United KingdomCell and Molecular Sciences, The James Hutton Institute, Dundee, United KingdomCell and Molecular Sciences, The James Hutton Institute, Dundee, United KingdomCell and Molecular Sciences, The James Hutton Institute, Dundee, United KingdomInformation and Computational Sciences, The James Hutton Institute, Dundee, United KingdomInformation and Computational Sciences, The James Hutton Institute, Dundee, United KingdomCell and Molecular Sciences, The James Hutton Institute, Dundee, United KingdomEnvironmental and Biochemical Sciences, The James Hutton Institute, Dundee, United KingdomCell and Molecular Sciences, The James Hutton Institute, Dundee, United KingdomCell and Molecular Sciences, The James Hutton Institute, Dundee, United KingdomBlackcurrant fruit collected at six stages of development were assessed for changes in gene expression using custom whole transcriptome microarrays and for variation in metabolite content using a combination of liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry. Principal components analysis demonstrated that fruit development could be clearly defined according to their transcript or metabolite profiles. During early developmental stages, metabolite profiles were dominated by amino acids and tannins, whilst transcript profiles were enriched in functions associated with cell division, anatomical structure morphogenesis and cell wall metabolism. During mid fruit development, fatty acids accumulated and transcript profiles were consistent with seed and embryo development. At the later stages, sugars and anthocyanins accumulated consistent with transcript profiles that were associated with secondary metabolism. Transcript data also indicated active signaling during later stages of fruit development. A targeted analysis of signaling networks revealed a dynamic activation and repression of almost 60 different transcripts encoding transcription factors across the course of fruit development, many of which have been demonstrated as pivotal to controlling such processes in other species. Transcripts associated with cytokinin and gibberellin were highly abundant at early fruit development, whilst those associated with ABA and ethylene tended to be more abundant at later stages. The data presented here provides an insight into fruit development in blackcurrant and provides a foundation for further work in the elucidation of the genetic basis of fruit quality.https://www.frontiersin.org/article/10.3389/fpls.2018.01235/fullmetabolomicsfruit ripeningnon-climacteric fruitorgan developmentendoreduplication |
| spellingShingle | Dorota A. Jarret Jenny Morris Danny W. Cullen Sandra L. Gordon Susan R. Verrall Linda Milne Pete E. Hedley J. William Allwood Rex M. Brennan Robert D. Hancock A Transcript and Metabolite Atlas of Blackcurrant Fruit Development Highlights Hormonal Regulation and Reveals the Role of Key Transcription Factors Frontiers in Plant Science metabolomics fruit ripening non-climacteric fruit organ development endoreduplication |
| title | A Transcript and Metabolite Atlas of Blackcurrant Fruit Development Highlights Hormonal Regulation and Reveals the Role of Key Transcription Factors |
| title_full | A Transcript and Metabolite Atlas of Blackcurrant Fruit Development Highlights Hormonal Regulation and Reveals the Role of Key Transcription Factors |
| title_fullStr | A Transcript and Metabolite Atlas of Blackcurrant Fruit Development Highlights Hormonal Regulation and Reveals the Role of Key Transcription Factors |
| title_full_unstemmed | A Transcript and Metabolite Atlas of Blackcurrant Fruit Development Highlights Hormonal Regulation and Reveals the Role of Key Transcription Factors |
| title_short | A Transcript and Metabolite Atlas of Blackcurrant Fruit Development Highlights Hormonal Regulation and Reveals the Role of Key Transcription Factors |
| title_sort | transcript and metabolite atlas of blackcurrant fruit development highlights hormonal regulation and reveals the role of key transcription factors |
| topic | metabolomics fruit ripening non-climacteric fruit organ development endoreduplication |
| url | https://www.frontiersin.org/article/10.3389/fpls.2018.01235/full |
| work_keys_str_mv | AT dorotaajarret atranscriptandmetaboliteatlasofblackcurrantfruitdevelopmenthighlightshormonalregulationandrevealstheroleofkeytranscriptionfactors AT jennymorris atranscriptandmetaboliteatlasofblackcurrantfruitdevelopmenthighlightshormonalregulationandrevealstheroleofkeytranscriptionfactors AT dannywcullen atranscriptandmetaboliteatlasofblackcurrantfruitdevelopmenthighlightshormonalregulationandrevealstheroleofkeytranscriptionfactors AT sandralgordon atranscriptandmetaboliteatlasofblackcurrantfruitdevelopmenthighlightshormonalregulationandrevealstheroleofkeytranscriptionfactors AT susanrverrall atranscriptandmetaboliteatlasofblackcurrantfruitdevelopmenthighlightshormonalregulationandrevealstheroleofkeytranscriptionfactors AT lindamilne atranscriptandmetaboliteatlasofblackcurrantfruitdevelopmenthighlightshormonalregulationandrevealstheroleofkeytranscriptionfactors AT peteehedley atranscriptandmetaboliteatlasofblackcurrantfruitdevelopmenthighlightshormonalregulationandrevealstheroleofkeytranscriptionfactors AT jwilliamallwood atranscriptandmetaboliteatlasofblackcurrantfruitdevelopmenthighlightshormonalregulationandrevealstheroleofkeytranscriptionfactors AT rexmbrennan atranscriptandmetaboliteatlasofblackcurrantfruitdevelopmenthighlightshormonalregulationandrevealstheroleofkeytranscriptionfactors AT robertdhancock atranscriptandmetaboliteatlasofblackcurrantfruitdevelopmenthighlightshormonalregulationandrevealstheroleofkeytranscriptionfactors AT dorotaajarret transcriptandmetaboliteatlasofblackcurrantfruitdevelopmenthighlightshormonalregulationandrevealstheroleofkeytranscriptionfactors AT jennymorris transcriptandmetaboliteatlasofblackcurrantfruitdevelopmenthighlightshormonalregulationandrevealstheroleofkeytranscriptionfactors AT dannywcullen transcriptandmetaboliteatlasofblackcurrantfruitdevelopmenthighlightshormonalregulationandrevealstheroleofkeytranscriptionfactors AT sandralgordon transcriptandmetaboliteatlasofblackcurrantfruitdevelopmenthighlightshormonalregulationandrevealstheroleofkeytranscriptionfactors AT susanrverrall transcriptandmetaboliteatlasofblackcurrantfruitdevelopmenthighlightshormonalregulationandrevealstheroleofkeytranscriptionfactors AT lindamilne transcriptandmetaboliteatlasofblackcurrantfruitdevelopmenthighlightshormonalregulationandrevealstheroleofkeytranscriptionfactors AT peteehedley transcriptandmetaboliteatlasofblackcurrantfruitdevelopmenthighlightshormonalregulationandrevealstheroleofkeytranscriptionfactors AT jwilliamallwood transcriptandmetaboliteatlasofblackcurrantfruitdevelopmenthighlightshormonalregulationandrevealstheroleofkeytranscriptionfactors AT rexmbrennan transcriptandmetaboliteatlasofblackcurrantfruitdevelopmenthighlightshormonalregulationandrevealstheroleofkeytranscriptionfactors AT robertdhancock transcriptandmetaboliteatlasofblackcurrantfruitdevelopmenthighlightshormonalregulationandrevealstheroleofkeytranscriptionfactors |