The origin of B chromosomes in yellow-necked mice (Apodemus flavicollis)-Break rules but keep playing the game.
B chromosomes (Bs) are known for more than hundred years but their origin, structure and pattern of evolution are not well understood. In the past few years new methodological approaches, involving isolation of Bs followed by whole DNA amplification, DNA probe generation, and fluorescent in situ hyb...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5362141?pdf=render |
| _version_ | 1828446195873218560 |
|---|---|
| author | M Rajičić S A Romanenko T V Karamysheva J Blagojević T Adnađević I Budinski A S Bogdanov V A Trifonov N B Rubtsov M Vujošević |
| author_facet | M Rajičić S A Romanenko T V Karamysheva J Blagojević T Adnađević I Budinski A S Bogdanov V A Trifonov N B Rubtsov M Vujošević |
| author_sort | M Rajičić |
| collection | DOAJ |
| description | B chromosomes (Bs) are known for more than hundred years but their origin, structure and pattern of evolution are not well understood. In the past few years new methodological approaches, involving isolation of Bs followed by whole DNA amplification, DNA probe generation, and fluorescent in situ hybridization (FISH) or the B chromosome DNA sequencing, has allowed detailed analysis of their origin and molecular structure in different species. In this study we explored the origin of Bs in the yellow-necked wood mouse, Apodemus flavicollis, using generation of microdissected DNA probes followed by FISH on metaphase chromosomes. Bs of A. flavicollis were successfully isolated and DNA was used as the template for B-specific probes for the first time. We revealed homology of DNA derived from the analyzed B chromosomes to the pericentromeric region (PR) of sex chromosomes and subtelomeric region of two pairs of small autosomes, but lower homology to the rest of the Y chromosome. Moreover, all analysed Bs had the same structure regardless of their number per individual or the great geographic distance between examined populations from the Balkan Peninsula (Serbia) and Eastern Europe (south region of Russia and central Belarus). Therefore, it was suggested that B chromosomes in A. flavicollis have a unique common origin from the PR of sex chromosomes, and/or similar evolutionary pattern. |
| first_indexed | 2024-12-10T22:08:28Z |
| format | Article |
| id | doaj.art-23d45b5d8b654f5eb9e39de69a6b3afd |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T22:08:28Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-23d45b5d8b654f5eb9e39de69a6b3afd2022-12-22T01:31:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01123e017270410.1371/journal.pone.0172704The origin of B chromosomes in yellow-necked mice (Apodemus flavicollis)-Break rules but keep playing the game.M RajičićS A RomanenkoT V KaramyshevaJ BlagojevićT AdnađevićI BudinskiA S BogdanovV A TrifonovN B RubtsovM VujoševićB chromosomes (Bs) are known for more than hundred years but their origin, structure and pattern of evolution are not well understood. In the past few years new methodological approaches, involving isolation of Bs followed by whole DNA amplification, DNA probe generation, and fluorescent in situ hybridization (FISH) or the B chromosome DNA sequencing, has allowed detailed analysis of their origin and molecular structure in different species. In this study we explored the origin of Bs in the yellow-necked wood mouse, Apodemus flavicollis, using generation of microdissected DNA probes followed by FISH on metaphase chromosomes. Bs of A. flavicollis were successfully isolated and DNA was used as the template for B-specific probes for the first time. We revealed homology of DNA derived from the analyzed B chromosomes to the pericentromeric region (PR) of sex chromosomes and subtelomeric region of two pairs of small autosomes, but lower homology to the rest of the Y chromosome. Moreover, all analysed Bs had the same structure regardless of their number per individual or the great geographic distance between examined populations from the Balkan Peninsula (Serbia) and Eastern Europe (south region of Russia and central Belarus). Therefore, it was suggested that B chromosomes in A. flavicollis have a unique common origin from the PR of sex chromosomes, and/or similar evolutionary pattern.http://europepmc.org/articles/PMC5362141?pdf=render |
| spellingShingle | M Rajičić S A Romanenko T V Karamysheva J Blagojević T Adnađević I Budinski A S Bogdanov V A Trifonov N B Rubtsov M Vujošević The origin of B chromosomes in yellow-necked mice (Apodemus flavicollis)-Break rules but keep playing the game. PLoS ONE |
| title | The origin of B chromosomes in yellow-necked mice (Apodemus flavicollis)-Break rules but keep playing the game. |
| title_full | The origin of B chromosomes in yellow-necked mice (Apodemus flavicollis)-Break rules but keep playing the game. |
| title_fullStr | The origin of B chromosomes in yellow-necked mice (Apodemus flavicollis)-Break rules but keep playing the game. |
| title_full_unstemmed | The origin of B chromosomes in yellow-necked mice (Apodemus flavicollis)-Break rules but keep playing the game. |
| title_short | The origin of B chromosomes in yellow-necked mice (Apodemus flavicollis)-Break rules but keep playing the game. |
| title_sort | origin of b chromosomes in yellow necked mice apodemus flavicollis break rules but keep playing the game |
| url | http://europepmc.org/articles/PMC5362141?pdf=render |
| work_keys_str_mv | AT mrajicic theoriginofbchromosomesinyellowneckedmiceapodemusflavicollisbreakrulesbutkeepplayingthegame AT saromanenko theoriginofbchromosomesinyellowneckedmiceapodemusflavicollisbreakrulesbutkeepplayingthegame AT tvkaramysheva theoriginofbchromosomesinyellowneckedmiceapodemusflavicollisbreakrulesbutkeepplayingthegame AT jblagojevic theoriginofbchromosomesinyellowneckedmiceapodemusflavicollisbreakrulesbutkeepplayingthegame AT tadnađevic theoriginofbchromosomesinyellowneckedmiceapodemusflavicollisbreakrulesbutkeepplayingthegame AT ibudinski theoriginofbchromosomesinyellowneckedmiceapodemusflavicollisbreakrulesbutkeepplayingthegame AT asbogdanov theoriginofbchromosomesinyellowneckedmiceapodemusflavicollisbreakrulesbutkeepplayingthegame AT vatrifonov theoriginofbchromosomesinyellowneckedmiceapodemusflavicollisbreakrulesbutkeepplayingthegame AT nbrubtsov theoriginofbchromosomesinyellowneckedmiceapodemusflavicollisbreakrulesbutkeepplayingthegame AT mvujosevic theoriginofbchromosomesinyellowneckedmiceapodemusflavicollisbreakrulesbutkeepplayingthegame AT mrajicic originofbchromosomesinyellowneckedmiceapodemusflavicollisbreakrulesbutkeepplayingthegame AT saromanenko originofbchromosomesinyellowneckedmiceapodemusflavicollisbreakrulesbutkeepplayingthegame AT tvkaramysheva originofbchromosomesinyellowneckedmiceapodemusflavicollisbreakrulesbutkeepplayingthegame AT jblagojevic originofbchromosomesinyellowneckedmiceapodemusflavicollisbreakrulesbutkeepplayingthegame AT tadnađevic originofbchromosomesinyellowneckedmiceapodemusflavicollisbreakrulesbutkeepplayingthegame AT ibudinski originofbchromosomesinyellowneckedmiceapodemusflavicollisbreakrulesbutkeepplayingthegame AT asbogdanov originofbchromosomesinyellowneckedmiceapodemusflavicollisbreakrulesbutkeepplayingthegame AT vatrifonov originofbchromosomesinyellowneckedmiceapodemusflavicollisbreakrulesbutkeepplayingthegame AT nbrubtsov originofbchromosomesinyellowneckedmiceapodemusflavicollisbreakrulesbutkeepplayingthegame AT mvujosevic originofbchromosomesinyellowneckedmiceapodemusflavicollisbreakrulesbutkeepplayingthegame |