Integration of conventional cell viability assays for reliable and reproducible read-outs: experimental evidence
Abstract Objective Short-term viability assays of cultured cells in 96-well plates are routinely used to determine the cytotoxicity or safety of drugs. These are often based on the formation of chromogen, generated selectively in viable cells. The innate problems of such short-term cell viability as...
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Format: | Article |
Language: | English |
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BMC
2018-06-01
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Series: | BMC Research Notes |
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Online Access: | http://link.springer.com/article/10.1186/s13104-018-3512-5 |
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author | Sukant Garg He Huifu Sunil C. Kaul Renu Wadhwa |
author_facet | Sukant Garg He Huifu Sunil C. Kaul Renu Wadhwa |
author_sort | Sukant Garg |
collection | DOAJ |
description | Abstract Objective Short-term viability assays of cultured cells in 96-well plates are routinely used to determine the cytotoxicity or safety of drugs. These are often based on the formation of chromogen, generated selectively in viable cells. The innate problems of such short-term cell viability assays include (i) effect of drugs is determined by cell density (ii) some drugs have slow/gradual effect and hence may escape such assays, (iii) cell morphology that reveal significant hints to molecular signaling underlining the effect of drugs cannot be effectively captured, (iv) long-term effect on viability and clonogenic potential of cells cannot be determined and (v) herbal extracts often possess intrinsic color that interferes with spectrophotometer estimation. In light of the ease and importance of cell culture-based assessment of drug safety and cytotoxicity, we attempted to combine the conventional cell-based assays in a way that allows multiple readouts (quantitative and qualitative) from a single experiment, and avoids the drawbacks of color interference. Results We have established and validated (using 16 types of cultured mammalian cells) a Quantitative and Qualitative Cell Viability assay in 12-well cell culture plates. It overcomes several shortcomings as discussed above and allows long-term observations on cell morphology and clonogenicity. |
first_indexed | 2024-04-12T03:56:54Z |
format | Article |
id | doaj.art-23d91ded7caa4642931002fab6e2d51d |
institution | Directory Open Access Journal |
issn | 1756-0500 |
language | English |
last_indexed | 2024-04-12T03:56:54Z |
publishDate | 2018-06-01 |
publisher | BMC |
record_format | Article |
series | BMC Research Notes |
spelling | doaj.art-23d91ded7caa4642931002fab6e2d51d2022-12-22T03:48:48ZengBMCBMC Research Notes1756-05002018-06-011111710.1186/s13104-018-3512-5Integration of conventional cell viability assays for reliable and reproducible read-outs: experimental evidenceSukant Garg0He Huifu1Sunil C. Kaul2Renu Wadhwa3DAILAB, DBT-AIST International Center for Translational and Environmental Research (DAICENTER), National Institute of Advanced Industrial Science & Technology (AIST)DAILAB, DBT-AIST International Center for Translational and Environmental Research (DAICENTER), National Institute of Advanced Industrial Science & Technology (AIST)DAILAB, DBT-AIST International Center for Translational and Environmental Research (DAICENTER), National Institute of Advanced Industrial Science & Technology (AIST)DAILAB, DBT-AIST International Center for Translational and Environmental Research (DAICENTER), National Institute of Advanced Industrial Science & Technology (AIST)Abstract Objective Short-term viability assays of cultured cells in 96-well plates are routinely used to determine the cytotoxicity or safety of drugs. These are often based on the formation of chromogen, generated selectively in viable cells. The innate problems of such short-term cell viability assays include (i) effect of drugs is determined by cell density (ii) some drugs have slow/gradual effect and hence may escape such assays, (iii) cell morphology that reveal significant hints to molecular signaling underlining the effect of drugs cannot be effectively captured, (iv) long-term effect on viability and clonogenic potential of cells cannot be determined and (v) herbal extracts often possess intrinsic color that interferes with spectrophotometer estimation. In light of the ease and importance of cell culture-based assessment of drug safety and cytotoxicity, we attempted to combine the conventional cell-based assays in a way that allows multiple readouts (quantitative and qualitative) from a single experiment, and avoids the drawbacks of color interference. Results We have established and validated (using 16 types of cultured mammalian cells) a Quantitative and Qualitative Cell Viability assay in 12-well cell culture plates. It overcomes several shortcomings as discussed above and allows long-term observations on cell morphology and clonogenicity.http://link.springer.com/article/10.1186/s13104-018-3512-5QCVCrystal violetClonogenicityQuantitative–qualitative assay |
spellingShingle | Sukant Garg He Huifu Sunil C. Kaul Renu Wadhwa Integration of conventional cell viability assays for reliable and reproducible read-outs: experimental evidence BMC Research Notes QCV Crystal violet Clonogenicity Quantitative–qualitative assay |
title | Integration of conventional cell viability assays for reliable and reproducible read-outs: experimental evidence |
title_full | Integration of conventional cell viability assays for reliable and reproducible read-outs: experimental evidence |
title_fullStr | Integration of conventional cell viability assays for reliable and reproducible read-outs: experimental evidence |
title_full_unstemmed | Integration of conventional cell viability assays for reliable and reproducible read-outs: experimental evidence |
title_short | Integration of conventional cell viability assays for reliable and reproducible read-outs: experimental evidence |
title_sort | integration of conventional cell viability assays for reliable and reproducible read outs experimental evidence |
topic | QCV Crystal violet Clonogenicity Quantitative–qualitative assay |
url | http://link.springer.com/article/10.1186/s13104-018-3512-5 |
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