Procyanidin B2 alleviates uterine toxicity induced by cadmium exposure in rats: The effect of oxidative stress, inflammation, and gut microbiota
Environmental exposure to hazardous materials causes enormous socioeconomic problems due to its deleterious impacts on human beings, agriculture and animal husbandry. As an important hazardous material, cadmium can promote uterine oxidative stress and inflammation, leading to reproductive toxicity....
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323007947 |
| _version_ | 1797683186238488576 |
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| author | Binhong Hu Songqing Liu Yuanyue Luo Jingyu Pu Xin Deng Wenjing Zhou Yuqing Dong Yichuan Ma Gang Wang Fan Yang Tianhui Zhu Jiasui Zhan |
| author_facet | Binhong Hu Songqing Liu Yuanyue Luo Jingyu Pu Xin Deng Wenjing Zhou Yuqing Dong Yichuan Ma Gang Wang Fan Yang Tianhui Zhu Jiasui Zhan |
| author_sort | Binhong Hu |
| collection | DOAJ |
| description | Environmental exposure to hazardous materials causes enormous socioeconomic problems due to its deleterious impacts on human beings, agriculture and animal husbandry. As an important hazardous material, cadmium can promote uterine oxidative stress and inflammation, leading to reproductive toxicity. Antioxidants have been reported to attenuate the reproductive toxicity associated with cadmium exposure. In this study, we investigated the potential protective effect of procyanidin oligosaccharide B2 (PC-B2) and gut microbiota on uterine toxicity induced by cadmium exposure in rats. The results showed that the expression levels of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) were reduced in utero. Proinflammatory cytokines (including tumor necrosis factor-α, interleukin-1β and interleukin-6), the NLRP3 inflammasome, Caspase-1 and pro-IL-1β were all involved in inflammatory-mediated uterine injury. PC-B2 prevented CdCl2-induced oxidative stress and inflammation in uterine tissue by increasing antioxidant enzymes and reducing proinflammatory cytokines. Additionally, PC-B2 significantly reduced cadmium deposition in the uterus, possibly through its significant increase in MT1, MT2, and MT3 mRNA expression. Interestingly, PC-B2 protected the uterus from CdCl2 damage by increasing the abundance of intestinal microbiota, promoting beneficial microbiota, and inhibiting harmful microbiota. This study provides novel mechanistic insights into the toxicity of environmental cadmium exposure and indicates that PC-B2 could be used in the prevention of cadmium exposure-induced uterine toxicity. |
| first_indexed | 2024-03-12T00:10:53Z |
| format | Article |
| id | doaj.art-23dc865745894f51a4f4e3f68189bb83 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-03-12T00:10:53Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-23dc865745894f51a4f4e3f68189bb832023-09-16T05:28:27ZengElsevierEcotoxicology and Environmental Safety0147-65132023-09-01263115290Procyanidin B2 alleviates uterine toxicity induced by cadmium exposure in rats: The effect of oxidative stress, inflammation, and gut microbiotaBinhong Hu0Songqing Liu1Yuanyue Luo2Jingyu Pu3Xin Deng4Wenjing Zhou5Yuqing Dong6Yichuan Ma7Gang Wang8Fan Yang9Tianhui Zhu10Jiasui Zhan11College of Chemistry and Life Sciences, Chengdu Normal University, Chengdu, China; Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources, Chengdu Normal University, Chengdu, China; Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences, Uppsala, SwedenCollege of Chemistry and Life Sciences, Chengdu Normal University, Chengdu, China; Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources, Chengdu Normal University, Chengdu, ChinaCollege of Chemistry and Life Sciences, Chengdu Normal University, Chengdu, ChinaCollege of Chemistry and Life Sciences, Chengdu Normal University, Chengdu, ChinaCollege of Chemistry and Life Sciences, Chengdu Normal University, Chengdu, ChinaCollege of Chemistry and Life Sciences, Chengdu Normal University, Chengdu, ChinaCollege of Forestry, Sichuan Agricultural University, Chengdu, ChinaCollege of Chemistry and Life Sciences, Chengdu Normal University, Chengdu, ChinaCollege of Chemistry and Life Sciences, Chengdu Normal University, Chengdu, China; Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources, Chengdu Normal University, Chengdu, ChinaCollege of Chemistry and Life Sciences, Chengdu Normal University, Chengdu, ChinaCollege of Forestry, Sichuan Agricultural University, Chengdu, China; Corresponding authors.Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences, Uppsala, Sweden; Corresponding authors.Environmental exposure to hazardous materials causes enormous socioeconomic problems due to its deleterious impacts on human beings, agriculture and animal husbandry. As an important hazardous material, cadmium can promote uterine oxidative stress and inflammation, leading to reproductive toxicity. Antioxidants have been reported to attenuate the reproductive toxicity associated with cadmium exposure. In this study, we investigated the potential protective effect of procyanidin oligosaccharide B2 (PC-B2) and gut microbiota on uterine toxicity induced by cadmium exposure in rats. The results showed that the expression levels of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) were reduced in utero. Proinflammatory cytokines (including tumor necrosis factor-α, interleukin-1β and interleukin-6), the NLRP3 inflammasome, Caspase-1 and pro-IL-1β were all involved in inflammatory-mediated uterine injury. PC-B2 prevented CdCl2-induced oxidative stress and inflammation in uterine tissue by increasing antioxidant enzymes and reducing proinflammatory cytokines. Additionally, PC-B2 significantly reduced cadmium deposition in the uterus, possibly through its significant increase in MT1, MT2, and MT3 mRNA expression. Interestingly, PC-B2 protected the uterus from CdCl2 damage by increasing the abundance of intestinal microbiota, promoting beneficial microbiota, and inhibiting harmful microbiota. This study provides novel mechanistic insights into the toxicity of environmental cadmium exposure and indicates that PC-B2 could be used in the prevention of cadmium exposure-induced uterine toxicity.http://www.sciencedirect.com/science/article/pii/S0147651323007947Procyanidin B2Cadmium exposureUterine toxicityRatsMicrobiome |
| spellingShingle | Binhong Hu Songqing Liu Yuanyue Luo Jingyu Pu Xin Deng Wenjing Zhou Yuqing Dong Yichuan Ma Gang Wang Fan Yang Tianhui Zhu Jiasui Zhan Procyanidin B2 alleviates uterine toxicity induced by cadmium exposure in rats: The effect of oxidative stress, inflammation, and gut microbiota Ecotoxicology and Environmental Safety Procyanidin B2 Cadmium exposure Uterine toxicity Rats Microbiome |
| title | Procyanidin B2 alleviates uterine toxicity induced by cadmium exposure in rats: The effect of oxidative stress, inflammation, and gut microbiota |
| title_full | Procyanidin B2 alleviates uterine toxicity induced by cadmium exposure in rats: The effect of oxidative stress, inflammation, and gut microbiota |
| title_fullStr | Procyanidin B2 alleviates uterine toxicity induced by cadmium exposure in rats: The effect of oxidative stress, inflammation, and gut microbiota |
| title_full_unstemmed | Procyanidin B2 alleviates uterine toxicity induced by cadmium exposure in rats: The effect of oxidative stress, inflammation, and gut microbiota |
| title_short | Procyanidin B2 alleviates uterine toxicity induced by cadmium exposure in rats: The effect of oxidative stress, inflammation, and gut microbiota |
| title_sort | procyanidin b2 alleviates uterine toxicity induced by cadmium exposure in rats the effect of oxidative stress inflammation and gut microbiota |
| topic | Procyanidin B2 Cadmium exposure Uterine toxicity Rats Microbiome |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323007947 |
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