Specific and label-free endogenous signature of dystrophic muscle by Synchrotron deep ultraviolet radiation
Abstract Dystrophic muscle is characterized by necrosis/regeneration cycles, inflammation, and fibro-adipogenic development. Conventional histological stainings provide essential topographical data of this remodeling but may be limited to discriminate closely related pathophysiological contexts. The...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-37762-1 |
| _version_ | 1797784609127137280 |
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| author | Laurence Dubreil Noreddine Damane Romain Fleurisson Marine Charrier Julien Pichon Isabelle Leroux Cindy Schleder Mireille Ledevin Thibaut Larcher Frédéric Jamme John Puentes Karl Rouger |
| author_facet | Laurence Dubreil Noreddine Damane Romain Fleurisson Marine Charrier Julien Pichon Isabelle Leroux Cindy Schleder Mireille Ledevin Thibaut Larcher Frédéric Jamme John Puentes Karl Rouger |
| author_sort | Laurence Dubreil |
| collection | DOAJ |
| description | Abstract Dystrophic muscle is characterized by necrosis/regeneration cycles, inflammation, and fibro-adipogenic development. Conventional histological stainings provide essential topographical data of this remodeling but may be limited to discriminate closely related pathophysiological contexts. They fail to mention microarchitecture changes linked to the nature and spatial distribution of tissue compartment components. We investigated whether label-free tissue autofluorescence revealed by Synchrotron deep ultraviolet (DUV) radiation could serve as an additional tool for monitoring dystrophic muscle remodeling. Using widefield microscopy with specific emission fluorescence filters and microspectroscopy defined by high spectral resolution, we analyzed samples from healthy dogs and two groups of dystrophic dogs: naïve (severely affected) and MuStem cell-transplanted (clinically stabilized) animals. Multivariate statistical analysis and machine learning approaches demonstrated that autofluorescence emitted at 420–480 nm by the Biceps femoris muscle effectively discriminates between healthy, dystrophic, and transplanted dog samples. Microspectroscopy showed that dystrophic dog muscle displays higher and lower autofluorescence due to collagen cross-linking and NADH respectively than that of healthy and transplanted dogs, defining biomarkers to evaluate the impact of cell transplantation. Our findings demonstrate that DUV radiation is a sensitive, label-free method to assess the histopathological status of dystrophic muscle using small amounts of tissue, with potential applications in regenerative medicine. |
| first_indexed | 2024-03-13T00:43:19Z |
| format | Article |
| id | doaj.art-23dcbe02f52448129a29f2379b79f39a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T00:43:19Z |
| publishDate | 2023-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-23dcbe02f52448129a29f2379b79f39a2023-07-09T11:11:59ZengNature PortfolioScientific Reports2045-23222023-07-0113111710.1038/s41598-023-37762-1Specific and label-free endogenous signature of dystrophic muscle by Synchrotron deep ultraviolet radiationLaurence Dubreil0Noreddine Damane1Romain Fleurisson2Marine Charrier3Julien Pichon4Isabelle Leroux5Cindy Schleder6Mireille Ledevin7Thibaut Larcher8Frédéric Jamme9John Puentes10Karl Rouger11Oniris, INRAE, PAnTherOniris, INRAE, PAnTherOniris, INRAE, PAnTherOniris, INRAE, PAnTherOniris, INRAE, PAnTherOniris, INRAE, PAnTherOniris, INRAE, PAnTherOniris, INRAE, PAnTherOniris, INRAE, PAnTherSynchrotron SOLEIL, BP48, L’Orme Des MerisiersIMT Atlantique, Lab-STICC, UMR CNRS 6285Oniris, INRAE, PAnTherAbstract Dystrophic muscle is characterized by necrosis/regeneration cycles, inflammation, and fibro-adipogenic development. Conventional histological stainings provide essential topographical data of this remodeling but may be limited to discriminate closely related pathophysiological contexts. They fail to mention microarchitecture changes linked to the nature and spatial distribution of tissue compartment components. We investigated whether label-free tissue autofluorescence revealed by Synchrotron deep ultraviolet (DUV) radiation could serve as an additional tool for monitoring dystrophic muscle remodeling. Using widefield microscopy with specific emission fluorescence filters and microspectroscopy defined by high spectral resolution, we analyzed samples from healthy dogs and two groups of dystrophic dogs: naïve (severely affected) and MuStem cell-transplanted (clinically stabilized) animals. Multivariate statistical analysis and machine learning approaches demonstrated that autofluorescence emitted at 420–480 nm by the Biceps femoris muscle effectively discriminates between healthy, dystrophic, and transplanted dog samples. Microspectroscopy showed that dystrophic dog muscle displays higher and lower autofluorescence due to collagen cross-linking and NADH respectively than that of healthy and transplanted dogs, defining biomarkers to evaluate the impact of cell transplantation. Our findings demonstrate that DUV radiation is a sensitive, label-free method to assess the histopathological status of dystrophic muscle using small amounts of tissue, with potential applications in regenerative medicine.https://doi.org/10.1038/s41598-023-37762-1 |
| spellingShingle | Laurence Dubreil Noreddine Damane Romain Fleurisson Marine Charrier Julien Pichon Isabelle Leroux Cindy Schleder Mireille Ledevin Thibaut Larcher Frédéric Jamme John Puentes Karl Rouger Specific and label-free endogenous signature of dystrophic muscle by Synchrotron deep ultraviolet radiation Scientific Reports |
| title | Specific and label-free endogenous signature of dystrophic muscle by Synchrotron deep ultraviolet radiation |
| title_full | Specific and label-free endogenous signature of dystrophic muscle by Synchrotron deep ultraviolet radiation |
| title_fullStr | Specific and label-free endogenous signature of dystrophic muscle by Synchrotron deep ultraviolet radiation |
| title_full_unstemmed | Specific and label-free endogenous signature of dystrophic muscle by Synchrotron deep ultraviolet radiation |
| title_short | Specific and label-free endogenous signature of dystrophic muscle by Synchrotron deep ultraviolet radiation |
| title_sort | specific and label free endogenous signature of dystrophic muscle by synchrotron deep ultraviolet radiation |
| url | https://doi.org/10.1038/s41598-023-37762-1 |
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