Comprehensive analysis of LAMC1 expression and prognostic value in kidney renal papillary cell carcinoma and clear cell carcinoma
Background: Laminin subunit gamma 1 (LAMC1) protein is associated with tumor cell invasion and metastasis. However, its role in kidney cancer remains unclear. In this work, we sought to probe the expression as well as its carcinogenic mechanisms of LAMC1 in kidney renal papillary cell carcinoma (KIR...
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| Format: | Article |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2022.988777/full |
| _version_ | 1828105094184304640 |
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| author | Jianrong Bai Axiu Zheng Yanping Ha Xiaoqing Xu Yaping Yu Yanda Lu Yanda Lu Shaojiang Zheng Shaojiang Zheng Zhihua Shen Botao Luo Wei Jie Wei Jie Wei Jie |
| author_facet | Jianrong Bai Axiu Zheng Yanping Ha Xiaoqing Xu Yaping Yu Yanda Lu Yanda Lu Shaojiang Zheng Shaojiang Zheng Zhihua Shen Botao Luo Wei Jie Wei Jie Wei Jie |
| author_sort | Jianrong Bai |
| collection | DOAJ |
| description | Background: Laminin subunit gamma 1 (LAMC1) protein is associated with tumor cell invasion and metastasis. However, its role in kidney cancer remains unclear. In this work, we sought to probe the expression as well as its carcinogenic mechanisms of LAMC1 in kidney renal papillary cell carcinoma (KIRP) and kidney renal clear cell carcinoma (KIRC).Methods: Public databases including TIMER, Oncomine, UALCAN, TISIDB, TCGA, Kaplan–Meier plotter, UCSC Xena, cBioPortal, SurvivalMeth, KEGG, GeneMANIA, Metascape, GSCALite and GDSC were adopted, and the expression, clinical pathological correlation, prognostic signatures, dominant factors influencing LAMC1 expression, DNA methylation levels, gene mutations, copy number variations, functional networks, and drug sensitivity were analyzed. Expression of LAMC1 protein in clinical KIRP and KIRC was validated using tissue array.Results:LAMC1 expression in KIRP and KIRC were significantly higher than those in normal tissues. High LAMC1 expression indicated poor overall survival in KIRP patients and better overall survival in KIRC patients. Through the univariate and multivariate Cox analysis, we found that high LAMC1 expression was a potential independent marker for poor prognosis in KIRP, however it implied a better prognosis in KIRC by univariate Cox analysis. In addition, the LAMC1 expression in KIRP and KIRC was negatively correlated with methylation levels of LAMC1 DNA. Interestingly, LAMC1 expression was positively correlated with the infiltration of CD8+ T cells, dendritic cells and neutrophils in KIRP; however, it was positively correlated with the infiltration of CD4+ T cells, macrophages and neutrophils but negatively correlated with B cells in KIRC. Moreover, high level of CD8+ T cells is beneficial for KIRC prognosis but opposite for KIRP. LAMC1 may participate in signaling pathways involved in formation of adherens junction and basement membrane in KIRP and KIRC, and the high expression of LAMC1 is resistant to most drugs or small molecules of the Genomics of Drug Sensitivity in Cancer database.Conclusion: Enhanced LAMC1 expression suggests a poor prognosis in KIRP while a better prognosis in KIRC, and these opposite prognostic signatures of LAMC1 may be related to different immune microenvironments. |
| first_indexed | 2024-04-11T09:56:57Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-04-11T09:56:57Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-23df1a700185434889a69bd8b43633ea2022-12-22T04:30:32ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2022-09-01910.3389/fmolb.2022.988777988777Comprehensive analysis of LAMC1 expression and prognostic value in kidney renal papillary cell carcinoma and clear cell carcinomaJianrong Bai0Axiu Zheng1Yanping Ha2Xiaoqing Xu3Yaping Yu4Yanda Lu5Yanda Lu6Shaojiang Zheng7Shaojiang Zheng8Zhihua Shen9Botao Luo10Wei Jie11Wei Jie12Wei Jie13Department of Pathology, School of Basic Medicine Sciences, Pathology Diagnosis and Research Center of Affiliated Hospital, Guangdong Medical University, Zhanjiang, ChinaDepartment of Pathology, School of Basic Medicine Sciences, Pathology Diagnosis and Research Center of Affiliated Hospital, Guangdong Medical University, Zhanjiang, ChinaDepartment of Pathology, School of Basic Medicine Sciences, Pathology Diagnosis and Research Center of Affiliated Hospital, Guangdong Medical University, Zhanjiang, ChinaCancer Institute of Hainan Medical University, Haikou, ChinaCancer Institute of Hainan Medical University, Haikou, ChinaCancer Institute of Hainan Medical University, Haikou, ChinaDepartment of Oncology, The First Affiliated Hospital, Hainan Medical University, Haikou, ChinaCancer Institute of Hainan Medical University, Haikou, ChinaDepartment of Oncology, The First Affiliated Hospital, Hainan Medical University, Haikou, ChinaDepartment of Pathology, School of Basic Medicine Sciences, Pathology Diagnosis and Research Center of Affiliated Hospital, Guangdong Medical University, Zhanjiang, ChinaDepartment of Pathology, School of Basic Medicine Sciences, Pathology Diagnosis and Research Center of Affiliated Hospital, Guangdong Medical University, Zhanjiang, ChinaDepartment of Pathology, School of Basic Medicine Sciences, Pathology Diagnosis and Research Center of Affiliated Hospital, Guangdong Medical University, Zhanjiang, ChinaCancer Institute of Hainan Medical University, Haikou, ChinaDepartment of Oncology, The First Affiliated Hospital, Hainan Medical University, Haikou, ChinaBackground: Laminin subunit gamma 1 (LAMC1) protein is associated with tumor cell invasion and metastasis. However, its role in kidney cancer remains unclear. In this work, we sought to probe the expression as well as its carcinogenic mechanisms of LAMC1 in kidney renal papillary cell carcinoma (KIRP) and kidney renal clear cell carcinoma (KIRC).Methods: Public databases including TIMER, Oncomine, UALCAN, TISIDB, TCGA, Kaplan–Meier plotter, UCSC Xena, cBioPortal, SurvivalMeth, KEGG, GeneMANIA, Metascape, GSCALite and GDSC were adopted, and the expression, clinical pathological correlation, prognostic signatures, dominant factors influencing LAMC1 expression, DNA methylation levels, gene mutations, copy number variations, functional networks, and drug sensitivity were analyzed. Expression of LAMC1 protein in clinical KIRP and KIRC was validated using tissue array.Results:LAMC1 expression in KIRP and KIRC were significantly higher than those in normal tissues. High LAMC1 expression indicated poor overall survival in KIRP patients and better overall survival in KIRC patients. Through the univariate and multivariate Cox analysis, we found that high LAMC1 expression was a potential independent marker for poor prognosis in KIRP, however it implied a better prognosis in KIRC by univariate Cox analysis. In addition, the LAMC1 expression in KIRP and KIRC was negatively correlated with methylation levels of LAMC1 DNA. Interestingly, LAMC1 expression was positively correlated with the infiltration of CD8+ T cells, dendritic cells and neutrophils in KIRP; however, it was positively correlated with the infiltration of CD4+ T cells, macrophages and neutrophils but negatively correlated with B cells in KIRC. Moreover, high level of CD8+ T cells is beneficial for KIRC prognosis but opposite for KIRP. LAMC1 may participate in signaling pathways involved in formation of adherens junction and basement membrane in KIRP and KIRC, and the high expression of LAMC1 is resistant to most drugs or small molecules of the Genomics of Drug Sensitivity in Cancer database.Conclusion: Enhanced LAMC1 expression suggests a poor prognosis in KIRP while a better prognosis in KIRC, and these opposite prognostic signatures of LAMC1 may be related to different immune microenvironments.https://www.frontiersin.org/articles/10.3389/fmolb.2022.988777/fullkidney renal papillary cell carcinoma (KIRP)kidney renal clear cell carcinoma (KIRC)LAMC1expressionprognosis |
| spellingShingle | Jianrong Bai Axiu Zheng Yanping Ha Xiaoqing Xu Yaping Yu Yanda Lu Yanda Lu Shaojiang Zheng Shaojiang Zheng Zhihua Shen Botao Luo Wei Jie Wei Jie Wei Jie Comprehensive analysis of LAMC1 expression and prognostic value in kidney renal papillary cell carcinoma and clear cell carcinoma Frontiers in Molecular Biosciences kidney renal papillary cell carcinoma (KIRP) kidney renal clear cell carcinoma (KIRC) LAMC1 expression prognosis |
| title | Comprehensive analysis of LAMC1 expression and prognostic value in kidney renal papillary cell carcinoma and clear cell carcinoma |
| title_full | Comprehensive analysis of LAMC1 expression and prognostic value in kidney renal papillary cell carcinoma and clear cell carcinoma |
| title_fullStr | Comprehensive analysis of LAMC1 expression and prognostic value in kidney renal papillary cell carcinoma and clear cell carcinoma |
| title_full_unstemmed | Comprehensive analysis of LAMC1 expression and prognostic value in kidney renal papillary cell carcinoma and clear cell carcinoma |
| title_short | Comprehensive analysis of LAMC1 expression and prognostic value in kidney renal papillary cell carcinoma and clear cell carcinoma |
| title_sort | comprehensive analysis of lamc1 expression and prognostic value in kidney renal papillary cell carcinoma and clear cell carcinoma |
| topic | kidney renal papillary cell carcinoma (KIRP) kidney renal clear cell carcinoma (KIRC) LAMC1 expression prognosis |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2022.988777/full |
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