Application of response surface methodology for optimization of medium components for the production of secondary metabolites by Streptomyces diastatochromogenes KX852460
Abstract A bioactive strain Streptomyces diastatochromogenes KX852460 was selected for the production of secondary metabolites to control the target spot disease on tobacco leaves, caused by the Rhizoctonia solani AG-3. Peanut meal, soluble starch, NaCl, yeast extract, and ammonium sulphate were ide...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2017-05-01
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| Series: | AMB Express |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13568-017-0388-z |
| _version_ | 1818693280076922880 |
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| author | Taswar Ahsan Jianguang Chen Yuanhua Wu Muhammad Irfan |
| author_facet | Taswar Ahsan Jianguang Chen Yuanhua Wu Muhammad Irfan |
| author_sort | Taswar Ahsan |
| collection | DOAJ |
| description | Abstract A bioactive strain Streptomyces diastatochromogenes KX852460 was selected for the production of secondary metabolites to control the target spot disease on tobacco leaves, caused by the Rhizoctonia solani AG-3. Peanut meal, soluble starch, NaCl, yeast extract, and ammonium sulphate were identified the best ingredient for high antifungal activity of S. diastatochromogenes KX852460 against the R. solani AG-3. For the improved production of secondary metabolites, central composite design of response surface methodology was applied in submerged fermentation. The best concentrations of ingredients were peanut meal 4.88%, soluble starch 4.40%, NaCl 0.52%, yeast extract 0.47%, and ammonium sulphate 0.0360%. Study of metabolism changes in the submerged fermentation process was analyzed. Level of the reducing sugar increased, as the total sugar consumed. Amino nitrogen and total sugar decrease tendency, which indicated the growth of bacteria in submerged fermentation batch. Production of secondary and other metabolites influenced the pH of the fermentation batch. |
| first_indexed | 2024-12-17T13:11:10Z |
| format | Article |
| id | doaj.art-23e30bf1979c4303b22ca589a795c672 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-17T13:11:10Z |
| publishDate | 2017-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-23e30bf1979c4303b22ca589a795c6722022-12-21T21:47:06ZengSpringerOpenAMB Express2191-08552017-05-017111010.1186/s13568-017-0388-zApplication of response surface methodology for optimization of medium components for the production of secondary metabolites by Streptomyces diastatochromogenes KX852460Taswar Ahsan0Jianguang Chen1Yuanhua Wu2Muhammad Irfan3Department of Plant Pathology, Plant Protection College, Shenyang Agricultural UniversityDepartment of Plant Pathology, Plant Protection College, Shenyang Agricultural UniversityDepartment of Plant Pathology, Plant Protection College, Shenyang Agricultural UniversityDepartment of Biotechnology, University of SargodhaAbstract A bioactive strain Streptomyces diastatochromogenes KX852460 was selected for the production of secondary metabolites to control the target spot disease on tobacco leaves, caused by the Rhizoctonia solani AG-3. Peanut meal, soluble starch, NaCl, yeast extract, and ammonium sulphate were identified the best ingredient for high antifungal activity of S. diastatochromogenes KX852460 against the R. solani AG-3. For the improved production of secondary metabolites, central composite design of response surface methodology was applied in submerged fermentation. The best concentrations of ingredients were peanut meal 4.88%, soluble starch 4.40%, NaCl 0.52%, yeast extract 0.47%, and ammonium sulphate 0.0360%. Study of metabolism changes in the submerged fermentation process was analyzed. Level of the reducing sugar increased, as the total sugar consumed. Amino nitrogen and total sugar decrease tendency, which indicated the growth of bacteria in submerged fermentation batch. Production of secondary and other metabolites influenced the pH of the fermentation batch.http://link.springer.com/article/10.1186/s13568-017-0388-zStreptomyces diastatochromogenesBioactive compoundsRSMFermentation |
| spellingShingle | Taswar Ahsan Jianguang Chen Yuanhua Wu Muhammad Irfan Application of response surface methodology for optimization of medium components for the production of secondary metabolites by Streptomyces diastatochromogenes KX852460 AMB Express Streptomyces diastatochromogenes Bioactive compounds RSM Fermentation |
| title | Application of response surface methodology for optimization of medium components for the production of secondary metabolites by Streptomyces diastatochromogenes KX852460 |
| title_full | Application of response surface methodology for optimization of medium components for the production of secondary metabolites by Streptomyces diastatochromogenes KX852460 |
| title_fullStr | Application of response surface methodology for optimization of medium components for the production of secondary metabolites by Streptomyces diastatochromogenes KX852460 |
| title_full_unstemmed | Application of response surface methodology for optimization of medium components for the production of secondary metabolites by Streptomyces diastatochromogenes KX852460 |
| title_short | Application of response surface methodology for optimization of medium components for the production of secondary metabolites by Streptomyces diastatochromogenes KX852460 |
| title_sort | application of response surface methodology for optimization of medium components for the production of secondary metabolites by streptomyces diastatochromogenes kx852460 |
| topic | Streptomyces diastatochromogenes Bioactive compounds RSM Fermentation |
| url | http://link.springer.com/article/10.1186/s13568-017-0388-z |
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