Antioxidant, antihyperglycemic, and antihyperlipidemic properties of Chimonanthus salicifolius S. Y. Hu leaves in experimental animals: modulation of thioredoxin and glutathione systems, renal water reabsorption, and gut microbiota
IntroductionExcessive calorie intake and physical inactivity have dramatically increased nutrient overload-associated disease, becoming a global public health issue. Chimonanthus salicifolius S. Y. Hu (CHI) is a homology plant of food and medicine in China and shows several health benefits.MethodsTh...
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2023.1168049/full |
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| author | Ruixia Dong Ruixia Dong Ruixia Dong Junjie Pan Guangshan Zhao Guangshan Zhao Qiuyan Zhao Shiqiong Wang Ning Li Lianjun Song Xianqing Huang Shuxing Miao Junhui Ying Fangying Wu Dongxu Wang Dongxu Wang Kejun Cheng Daniel Granato Qiuyan Ban |
| author_facet | Ruixia Dong Ruixia Dong Ruixia Dong Junjie Pan Guangshan Zhao Guangshan Zhao Qiuyan Zhao Shiqiong Wang Ning Li Lianjun Song Xianqing Huang Shuxing Miao Junhui Ying Fangying Wu Dongxu Wang Dongxu Wang Kejun Cheng Daniel Granato Qiuyan Ban |
| author_sort | Ruixia Dong |
| collection | DOAJ |
| description | IntroductionExcessive calorie intake and physical inactivity have dramatically increased nutrient overload-associated disease, becoming a global public health issue. Chimonanthus salicifolius S. Y. Hu (CHI) is a homology plant of food and medicine in China and shows several health benefits.MethodsThis work investigated the antioxidant activity, the alleviating effects, and the mechanism of action on diabetes and hyperlipidemia of CHI leaves.Results and discussionResults showed that CHI leaves infusion displayed in vitro antioxidant activity measured by ABTS and ferric reducing antioxidant power methods. In wild-type Kunming mice, CHI leaves infusion consumption activated the hepatic antioxidant enzymes, including glutathione reductase, glutathione S-transferase, glutathione peroxidase and thioredoxin reductase as well as thioredoxin reductase 1. In alloxan-induced type 1 diabetic mice, CHI leaves infusion ameliorated diabetic symptoms, including polyuria, polydipsia, polyphagia and hyperglycemia, in a dose-dependent and time-course manners. The mechanism involved CHI leaves up-regulating renal water reabsorption associated protein – urine transporter A1–and promoting the trafficking of urine transporter A1 and aquaporin 2 to the apical plasma membrane. Despite this, in high-fat diet-induced hyperlipidemic golden hamsters, CHI leaves powder did not significantly effect on hyperlipidemia and body weight gain. This might be attributed to CHI leaves powder increasing the calorie intake. Interestingly, we found that CHI leaves extract containing a lower dose of total flavonoid than CHI leaves powder pronouncedly reduced the levels of total cholesterol, triglyceride, and low-density lipoprotein cholesterol in serum in golden hamsters fed a high-fat diet. Furthermore, CHI leaves extract elevated the diversity of gut microbiota and the abundance of Bifidobacterium and Ruminococcaceae_UCG-014. It also decreased the abundance of Lactobacillus at the genus level in golden hamsters fed a high-fat diet. Overall, CHI leaves benefit oxidative stress prevention and metabolic syndrome amelioration in vivo. |
| first_indexed | 2024-04-09T15:27:39Z |
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| id | doaj.art-23e44fea5e664971a3564841961fa11f |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-09T15:27:39Z |
| publishDate | 2023-04-01 |
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| series | Frontiers in Nutrition |
| spelling | doaj.art-23e44fea5e664971a3564841961fa11f2023-04-28T10:49:55ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2023-04-011010.3389/fnut.2023.11680491168049Antioxidant, antihyperglycemic, and antihyperlipidemic properties of Chimonanthus salicifolius S. Y. Hu leaves in experimental animals: modulation of thioredoxin and glutathione systems, renal water reabsorption, and gut microbiotaRuixia Dong0Ruixia Dong1Ruixia Dong2Junjie Pan3Guangshan Zhao4Guangshan Zhao5Qiuyan Zhao6Shiqiong Wang7Ning Li8Lianjun Song9Xianqing Huang10Shuxing Miao11Junhui Ying12Fangying Wu13Dongxu Wang14Dongxu Wang15Kejun Cheng16Daniel Granato17Qiuyan Ban18College of Horticulture, Jinling Institute of Technology, Nanjing, ChinaCollege of Forestry Science and Technology, Lishui Vocational and Technical College, Lishui, ChinaState Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science and Technology, Anhui Agricultural University, Hefei, ChinaChemical Biology Center, Lishui Institute of Agriculture and Forestry Sciences, Lishui, ChinaState Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science and Technology, Anhui Agricultural University, Hefei, ChinaInnovation Team of Food Nutrition and Safety Control, College of Food Science and Technology, Henan Agricultural University, Zhengzhou, ChinaInnovation Team of Food Nutrition and Safety Control, College of Food Science and Technology, Henan Agricultural University, Zhengzhou, ChinaInnovation Team of Food Nutrition and Safety Control, College of Food Science and Technology, Henan Agricultural University, Zhengzhou, ChinaInnovation Team of Food Nutrition and Safety Control, College of Food Science and Technology, Henan Agricultural University, Zhengzhou, ChinaInnovation Team of Food Nutrition and Safety Control, College of Food Science and Technology, Henan Agricultural University, Zhengzhou, ChinaInnovation Team of Food Nutrition and Safety Control, College of Food Science and Technology, Henan Agricultural University, Zhengzhou, ChinaCollege of Horticulture, Jinling Institute of Technology, Nanjing, ChinaCollege of Forestry Science and Technology, Lishui Vocational and Technical College, Lishui, ChinaCollege of Forestry Science and Technology, Lishui Vocational and Technical College, Lishui, ChinaState Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science and Technology, Anhui Agricultural University, Hefei, ChinaSchool of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang, ChinaChemical Biology Center, Lishui Institute of Agriculture and Forestry Sciences, Lishui, ChinaBioactivity and Applications Lab, Department of Biological Sciences, Faculty of Science and Engineering, University of Limerick, Limerick, IrelandDepartment of Tea Science, College of Horticulture, Henan Agricultural University, Zhengzhou, ChinaIntroductionExcessive calorie intake and physical inactivity have dramatically increased nutrient overload-associated disease, becoming a global public health issue. Chimonanthus salicifolius S. Y. Hu (CHI) is a homology plant of food and medicine in China and shows several health benefits.MethodsThis work investigated the antioxidant activity, the alleviating effects, and the mechanism of action on diabetes and hyperlipidemia of CHI leaves.Results and discussionResults showed that CHI leaves infusion displayed in vitro antioxidant activity measured by ABTS and ferric reducing antioxidant power methods. In wild-type Kunming mice, CHI leaves infusion consumption activated the hepatic antioxidant enzymes, including glutathione reductase, glutathione S-transferase, glutathione peroxidase and thioredoxin reductase as well as thioredoxin reductase 1. In alloxan-induced type 1 diabetic mice, CHI leaves infusion ameliorated diabetic symptoms, including polyuria, polydipsia, polyphagia and hyperglycemia, in a dose-dependent and time-course manners. The mechanism involved CHI leaves up-regulating renal water reabsorption associated protein – urine transporter A1–and promoting the trafficking of urine transporter A1 and aquaporin 2 to the apical plasma membrane. Despite this, in high-fat diet-induced hyperlipidemic golden hamsters, CHI leaves powder did not significantly effect on hyperlipidemia and body weight gain. This might be attributed to CHI leaves powder increasing the calorie intake. Interestingly, we found that CHI leaves extract containing a lower dose of total flavonoid than CHI leaves powder pronouncedly reduced the levels of total cholesterol, triglyceride, and low-density lipoprotein cholesterol in serum in golden hamsters fed a high-fat diet. Furthermore, CHI leaves extract elevated the diversity of gut microbiota and the abundance of Bifidobacterium and Ruminococcaceae_UCG-014. It also decreased the abundance of Lactobacillus at the genus level in golden hamsters fed a high-fat diet. Overall, CHI leaves benefit oxidative stress prevention and metabolic syndrome amelioration in vivo.https://www.frontiersin.org/articles/10.3389/fnut.2023.1168049/fullChimonanthus salicifolius S. Y. Hu leavesantioxidantmetabolic syndromerenal water reabsorptiongut microbiota |
| spellingShingle | Ruixia Dong Ruixia Dong Ruixia Dong Junjie Pan Guangshan Zhao Guangshan Zhao Qiuyan Zhao Shiqiong Wang Ning Li Lianjun Song Xianqing Huang Shuxing Miao Junhui Ying Fangying Wu Dongxu Wang Dongxu Wang Kejun Cheng Daniel Granato Qiuyan Ban Antioxidant, antihyperglycemic, and antihyperlipidemic properties of Chimonanthus salicifolius S. Y. Hu leaves in experimental animals: modulation of thioredoxin and glutathione systems, renal water reabsorption, and gut microbiota Frontiers in Nutrition Chimonanthus salicifolius S. Y. Hu leaves antioxidant metabolic syndrome renal water reabsorption gut microbiota |
| title | Antioxidant, antihyperglycemic, and antihyperlipidemic properties of Chimonanthus salicifolius S. Y. Hu leaves in experimental animals: modulation of thioredoxin and glutathione systems, renal water reabsorption, and gut microbiota |
| title_full | Antioxidant, antihyperglycemic, and antihyperlipidemic properties of Chimonanthus salicifolius S. Y. Hu leaves in experimental animals: modulation of thioredoxin and glutathione systems, renal water reabsorption, and gut microbiota |
| title_fullStr | Antioxidant, antihyperglycemic, and antihyperlipidemic properties of Chimonanthus salicifolius S. Y. Hu leaves in experimental animals: modulation of thioredoxin and glutathione systems, renal water reabsorption, and gut microbiota |
| title_full_unstemmed | Antioxidant, antihyperglycemic, and antihyperlipidemic properties of Chimonanthus salicifolius S. Y. Hu leaves in experimental animals: modulation of thioredoxin and glutathione systems, renal water reabsorption, and gut microbiota |
| title_short | Antioxidant, antihyperglycemic, and antihyperlipidemic properties of Chimonanthus salicifolius S. Y. Hu leaves in experimental animals: modulation of thioredoxin and glutathione systems, renal water reabsorption, and gut microbiota |
| title_sort | antioxidant antihyperglycemic and antihyperlipidemic properties of chimonanthus salicifolius s y hu leaves in experimental animals modulation of thioredoxin and glutathione systems renal water reabsorption and gut microbiota |
| topic | Chimonanthus salicifolius S. Y. Hu leaves antioxidant metabolic syndrome renal water reabsorption gut microbiota |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2023.1168049/full |
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