Metagenomic next-generation sequencing to investigate infectious keratitis by Corynespora cassiicola: a case report
In this report, the case of a 65-year-old immunosuppressed female who presented with recurring redness and irritation in her right eye for 2 months is described. Ocular examination revealed conjunctival congestion, feather-like greyish-white corneal deep stromal infiltrate, white, floccular material...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2023.1285753/full |
| _version_ | 1827750223109160960 |
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| author | Shuo Xu Shui Lu Yan Gu Hongjuan Sun Minghui Ma Yue Leng Wenhui Liu |
| author_facet | Shuo Xu Shui Lu Yan Gu Hongjuan Sun Minghui Ma Yue Leng Wenhui Liu |
| author_sort | Shuo Xu |
| collection | DOAJ |
| description | In this report, the case of a 65-year-old immunosuppressed female who presented with recurring redness and irritation in her right eye for 2 months is described. Ocular examination revealed conjunctival congestion, feather-like greyish-white corneal deep stromal infiltrate, white, floccular material sprawling from the anterior chamber angle and hypopyon. The in vivo confocal microscopy (IVCM) instantly confirmed fungal keratitis, and empirical antifungal therapy was thus administered. The patient exhibited therapeutic penetrating keratoplasty, however, due to the progression of infection and the lack of identified pathogens. The fungal isolate was identified as Corynespora cassiicola by metagenomic next-generation sequencing (mNGS) of the host cornea. The patient responded well to intensive conservative therapy and subsequent surgical therapy. To our knowledge, this case represents the first case of C. cassiicola infection from China, highlighting the emergence of a rare fungus that causes keratitis. Furthermore, mNGS has the capability to facilitate prompt identification and timely management of challenging ocular infections that are difficult to diagnose. |
| first_indexed | 2024-03-11T06:40:36Z |
| format | Article |
| id | doaj.art-23e59330f2b94acfbbd44f7d092b738f |
| institution | Directory Open Access Journal |
| issn | 2296-858X |
| language | English |
| last_indexed | 2024-03-11T06:40:36Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj.art-23e59330f2b94acfbbd44f7d092b738f2023-11-17T10:37:11ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2023-11-011010.3389/fmed.2023.12857531285753Metagenomic next-generation sequencing to investigate infectious keratitis by Corynespora cassiicola: a case reportShuo Xu0Shui Lu1Yan Gu2Hongjuan Sun3Minghui Ma4Yue Leng5Wenhui Liu6Department of Ophthalmology, Jiangnan University Medical Center, Wuxi, ChinaDepartment of Ophthalmology, Jiangnan University Medical Center, Wuxi, ChinaDepartment of Ophthalmology, Jiangnan University Medical Center, Wuxi, ChinaDepartment of Ophthalmology, Jiangnan University Medical Center, Wuxi, ChinaDepartment of Drug Clinical Trial Institution, Jiangnan University Medical Center, Wuxi, ChinaDepartment of Human Resources Division, The Affiliated Wuxi People’s Nanjing Medical University, Wuxi, ChinaDepartment of Ophthalmology, Jiangnan University Medical Center, Wuxi, ChinaIn this report, the case of a 65-year-old immunosuppressed female who presented with recurring redness and irritation in her right eye for 2 months is described. Ocular examination revealed conjunctival congestion, feather-like greyish-white corneal deep stromal infiltrate, white, floccular material sprawling from the anterior chamber angle and hypopyon. The in vivo confocal microscopy (IVCM) instantly confirmed fungal keratitis, and empirical antifungal therapy was thus administered. The patient exhibited therapeutic penetrating keratoplasty, however, due to the progression of infection and the lack of identified pathogens. The fungal isolate was identified as Corynespora cassiicola by metagenomic next-generation sequencing (mNGS) of the host cornea. The patient responded well to intensive conservative therapy and subsequent surgical therapy. To our knowledge, this case represents the first case of C. cassiicola infection from China, highlighting the emergence of a rare fungus that causes keratitis. Furthermore, mNGS has the capability to facilitate prompt identification and timely management of challenging ocular infections that are difficult to diagnose.https://www.frontiersin.org/articles/10.3389/fmed.2023.1285753/fullCorynespora cassiicolafungal keratitiskeratoplastyvoriconazolemetagenomic next-generation sequencing |
| spellingShingle | Shuo Xu Shui Lu Yan Gu Hongjuan Sun Minghui Ma Yue Leng Wenhui Liu Metagenomic next-generation sequencing to investigate infectious keratitis by Corynespora cassiicola: a case report Frontiers in Medicine Corynespora cassiicola fungal keratitis keratoplasty voriconazole metagenomic next-generation sequencing |
| title | Metagenomic next-generation sequencing to investigate infectious keratitis by Corynespora cassiicola: a case report |
| title_full | Metagenomic next-generation sequencing to investigate infectious keratitis by Corynespora cassiicola: a case report |
| title_fullStr | Metagenomic next-generation sequencing to investigate infectious keratitis by Corynespora cassiicola: a case report |
| title_full_unstemmed | Metagenomic next-generation sequencing to investigate infectious keratitis by Corynespora cassiicola: a case report |
| title_short | Metagenomic next-generation sequencing to investigate infectious keratitis by Corynespora cassiicola: a case report |
| title_sort | metagenomic next generation sequencing to investigate infectious keratitis by corynespora cassiicola a case report |
| topic | Corynespora cassiicola fungal keratitis keratoplasty voriconazole metagenomic next-generation sequencing |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2023.1285753/full |
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