Effect of Resveratrol on Mitochondrial Biogenesis and Muscle Fiber Type Transformation in Bovine Myotubes via SIRT1/PGC-1α
This study investigated the effect of resveratrol on muscle fiber type transformation in bovine myotubes. Cell viability and related metabolic enzyme activities were measured by methyl thiazolyl tetrazolium (MTT) and colorimetric assays, and myogenic regulatory factors (MRFs), myosin heavy chains (M...
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| Format: | Article |
| Language: | English |
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China Food Publishing Company
2024-02-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-4-001.pdf |
| _version_ | 1797256607215648768 |
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| author | ZHANG Jingyue, DONG Pengcheng, ZUO Huixin, LIANG Rongrong, MAO Yanwei, ZHANG Yimin, YANG Xiaoyin, LUO Xin, ZHU Lixian |
| author_facet | ZHANG Jingyue, DONG Pengcheng, ZUO Huixin, LIANG Rongrong, MAO Yanwei, ZHANG Yimin, YANG Xiaoyin, LUO Xin, ZHU Lixian |
| author_sort | ZHANG Jingyue, DONG Pengcheng, ZUO Huixin, LIANG Rongrong, MAO Yanwei, ZHANG Yimin, YANG Xiaoyin, LUO Xin, ZHU Lixian |
| collection | DOAJ |
| description | This study investigated the effect of resveratrol on muscle fiber type transformation in bovine myotubes. Cell viability and related metabolic enzyme activities were measured by methyl thiazolyl tetrazolium (MTT) and colorimetric assays, and myogenic regulatory factors (MRFs), myosin heavy chains (MyHCs), and gene and protein expression related to mitochondrial biogenesis were determined. The results showed that resveratrol treatment significantly increased the gene expression of Myf5, Myf6, MyoG, and MyoD (P < 0.05) and promoted the differentiation of bovine myotubes. Resveratrol significantly increased slow MyHC expression and decreased fast MyHC expression, while upregulating the mRNA expression levels of MyHC I and MyHC IIa and downregulating the mRNA expression levels of MyHC IIx and MyHC IIb (P < 0.05). Resveratrol also significantly increased succinate dehydrogenase (SDH) and malate dehydrogenase (MDH) activity and decreased lactic dehydrogenase (LDH) activity (P < 0.05). In addition, resveratrol increased the gene and protein expression levels of silent information regulator 1 (SIRT1), peroxisome proliferator-activated receptor-gamma coactivator-1α (PGC-1α), nucleus respiratory factors-1 (NRF-1), mitochondrial transcription factor A (TFAM) (P < 0.05). Addition of the SIRT1 inhibitor 6-chloro-2,3,4,9-tetrahydro-1H-carbazole-1-carboxamide (EX527) significantly attenuated resveratrol-induced muscle fiber type transformation (P < 0.05), and the promoting effect of resveratrol on the gene and protein expression of SIRT1, PGC-1α, NRF-1, and TFAM (P < 0.05). Taken together, resveratrol can promote mitochondrial biogenesis and consequently muscle fiber type transformation by activating the SIRT1/PGC-1α signaling pathway. |
| first_indexed | 2024-04-24T22:24:26Z |
| format | Article |
| id | doaj.art-23e69f45219241d1b89660ecd3e5f293 |
| institution | Directory Open Access Journal |
| issn | 1002-6630 |
| language | English |
| last_indexed | 2024-04-24T22:24:26Z |
| publishDate | 2024-02-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj.art-23e69f45219241d1b89660ecd3e5f2932024-03-20T02:27:16ZengChina Food Publishing CompanyShipin Kexue1002-66302024-02-014541910.7506/spkx1002-6630-20230527-256Effect of Resveratrol on Mitochondrial Biogenesis and Muscle Fiber Type Transformation in Bovine Myotubes via SIRT1/PGC-1αZHANG Jingyue, DONG Pengcheng, ZUO Huixin, LIANG Rongrong, MAO Yanwei, ZHANG Yimin, YANG Xiaoyin, LUO Xin, ZHU Lixian0(National R & D Center for Beef Processing Technology, College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, China)This study investigated the effect of resveratrol on muscle fiber type transformation in bovine myotubes. Cell viability and related metabolic enzyme activities were measured by methyl thiazolyl tetrazolium (MTT) and colorimetric assays, and myogenic regulatory factors (MRFs), myosin heavy chains (MyHCs), and gene and protein expression related to mitochondrial biogenesis were determined. The results showed that resveratrol treatment significantly increased the gene expression of Myf5, Myf6, MyoG, and MyoD (P < 0.05) and promoted the differentiation of bovine myotubes. Resveratrol significantly increased slow MyHC expression and decreased fast MyHC expression, while upregulating the mRNA expression levels of MyHC I and MyHC IIa and downregulating the mRNA expression levels of MyHC IIx and MyHC IIb (P < 0.05). Resveratrol also significantly increased succinate dehydrogenase (SDH) and malate dehydrogenase (MDH) activity and decreased lactic dehydrogenase (LDH) activity (P < 0.05). In addition, resveratrol increased the gene and protein expression levels of silent information regulator 1 (SIRT1), peroxisome proliferator-activated receptor-gamma coactivator-1α (PGC-1α), nucleus respiratory factors-1 (NRF-1), mitochondrial transcription factor A (TFAM) (P < 0.05). Addition of the SIRT1 inhibitor 6-chloro-2,3,4,9-tetrahydro-1H-carbazole-1-carboxamide (EX527) significantly attenuated resveratrol-induced muscle fiber type transformation (P < 0.05), and the promoting effect of resveratrol on the gene and protein expression of SIRT1, PGC-1α, NRF-1, and TFAM (P < 0.05). Taken together, resveratrol can promote mitochondrial biogenesis and consequently muscle fiber type transformation by activating the SIRT1/PGC-1α signaling pathway.https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-4-001.pdfresveratrol; bovine myotubes; silent information regulator 1/peroxisome proliferator-activated receptor-gamma coactivator-1α; muscle fiber type transformation; mitochondrial biogenesis |
| spellingShingle | ZHANG Jingyue, DONG Pengcheng, ZUO Huixin, LIANG Rongrong, MAO Yanwei, ZHANG Yimin, YANG Xiaoyin, LUO Xin, ZHU Lixian Effect of Resveratrol on Mitochondrial Biogenesis and Muscle Fiber Type Transformation in Bovine Myotubes via SIRT1/PGC-1α Shipin Kexue resveratrol; bovine myotubes; silent information regulator 1/peroxisome proliferator-activated receptor-gamma coactivator-1α; muscle fiber type transformation; mitochondrial biogenesis |
| title | Effect of Resveratrol on Mitochondrial Biogenesis and Muscle Fiber Type Transformation in Bovine Myotubes via SIRT1/PGC-1α |
| title_full | Effect of Resveratrol on Mitochondrial Biogenesis and Muscle Fiber Type Transformation in Bovine Myotubes via SIRT1/PGC-1α |
| title_fullStr | Effect of Resveratrol on Mitochondrial Biogenesis and Muscle Fiber Type Transformation in Bovine Myotubes via SIRT1/PGC-1α |
| title_full_unstemmed | Effect of Resveratrol on Mitochondrial Biogenesis and Muscle Fiber Type Transformation in Bovine Myotubes via SIRT1/PGC-1α |
| title_short | Effect of Resveratrol on Mitochondrial Biogenesis and Muscle Fiber Type Transformation in Bovine Myotubes via SIRT1/PGC-1α |
| title_sort | effect of resveratrol on mitochondrial biogenesis and muscle fiber type transformation in bovine myotubes via sirt1 pgc 1α |
| topic | resveratrol; bovine myotubes; silent information regulator 1/peroxisome proliferator-activated receptor-gamma coactivator-1α; muscle fiber type transformation; mitochondrial biogenesis |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-4-001.pdf |
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