Polygonum barbatum extract reduces colorectal cancer cell proliferation, migration, invasion, and epithelial–mesenchymal transition via YAP and β-catenin pathway regulation
Abstract Colorectal cancer (CRC) is the third most common cancer worldwide with novel therapeutic developmental challenges. Polygonum barbatum has anticancer potential, but its mechanism(s) are unclear. This study investigates the inhibitory effect of P. barbatum on human CRC cells. Polygonum barbat...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-45630-1 |
| _version_ | 1797647242377560064 |
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| author | Pi-Kai Chang I-Chuan Yen Wei-Cheng Tsai Shih-Yu Lee |
| author_facet | Pi-Kai Chang I-Chuan Yen Wei-Cheng Tsai Shih-Yu Lee |
| author_sort | Pi-Kai Chang |
| collection | DOAJ |
| description | Abstract Colorectal cancer (CRC) is the third most common cancer worldwide with novel therapeutic developmental challenges. Polygonum barbatum has anticancer potential, but its mechanism(s) are unclear. This study investigates the inhibitory effect of P. barbatum on human CRC cells. Polygonum barbatum extract (PBE) and quercetin standard HPLC fingerprints were determined using analytical RP-HPLC and evaluations were completed using the human colon cancer cell line HCT-116 (KRASG13D mutation) and HT-29 (BRAF mutation) cells. Post-PBE treatment, cell viability, colony formation, migration, invasion, and apoptosis, as well as changes in the whole-transcriptome of cells were analyzed. PBE significantly reduced CRC cell growth, migration, and invasion, and the genes responsible for extracellular matrix (ECM) organization, cell motility, and cell growth were suppressed by PBE. The differentially expressed genes revealed that PBE treatment exerted a significant effect on the ECM interaction and focal adhesion pathways. Epithelial-to-mesenchymal transition markers, N-cadherin, vimentin, SLUG, and SNAIL, were shown to be regulated by PBE. These effects were associated with blockade of the Yes-associated protein and the GSK3β/β-catenin axis. PBE exerts a significant inhibitory effect on CRC cells and may be applicable in clinical trials. |
| first_indexed | 2024-03-11T15:14:30Z |
| format | Article |
| id | doaj.art-23e6a526042948b4adc4e43d33c1efe2 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-11T15:14:30Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-23e6a526042948b4adc4e43d33c1efe22023-10-29T12:24:24ZengNature PortfolioScientific Reports2045-23222023-10-0113111410.1038/s41598-023-45630-1Polygonum barbatum extract reduces colorectal cancer cell proliferation, migration, invasion, and epithelial–mesenchymal transition via YAP and β-catenin pathway regulationPi-Kai Chang0I-Chuan Yen1Wei-Cheng Tsai2Shih-Yu Lee3Graduate Institute of Medical Sciences, National Defense Medical CenterSchool of Pharmacy, National Defense Medical CenterGraduate Institute of Aerospace and Undersea Medicine, National Defense Medical CenterGraduate Institute of Medical Sciences, National Defense Medical CenterAbstract Colorectal cancer (CRC) is the third most common cancer worldwide with novel therapeutic developmental challenges. Polygonum barbatum has anticancer potential, but its mechanism(s) are unclear. This study investigates the inhibitory effect of P. barbatum on human CRC cells. Polygonum barbatum extract (PBE) and quercetin standard HPLC fingerprints were determined using analytical RP-HPLC and evaluations were completed using the human colon cancer cell line HCT-116 (KRASG13D mutation) and HT-29 (BRAF mutation) cells. Post-PBE treatment, cell viability, colony formation, migration, invasion, and apoptosis, as well as changes in the whole-transcriptome of cells were analyzed. PBE significantly reduced CRC cell growth, migration, and invasion, and the genes responsible for extracellular matrix (ECM) organization, cell motility, and cell growth were suppressed by PBE. The differentially expressed genes revealed that PBE treatment exerted a significant effect on the ECM interaction and focal adhesion pathways. Epithelial-to-mesenchymal transition markers, N-cadherin, vimentin, SLUG, and SNAIL, were shown to be regulated by PBE. These effects were associated with blockade of the Yes-associated protein and the GSK3β/β-catenin axis. PBE exerts a significant inhibitory effect on CRC cells and may be applicable in clinical trials.https://doi.org/10.1038/s41598-023-45630-1 |
| spellingShingle | Pi-Kai Chang I-Chuan Yen Wei-Cheng Tsai Shih-Yu Lee Polygonum barbatum extract reduces colorectal cancer cell proliferation, migration, invasion, and epithelial–mesenchymal transition via YAP and β-catenin pathway regulation Scientific Reports |
| title | Polygonum barbatum extract reduces colorectal cancer cell proliferation, migration, invasion, and epithelial–mesenchymal transition via YAP and β-catenin pathway regulation |
| title_full | Polygonum barbatum extract reduces colorectal cancer cell proliferation, migration, invasion, and epithelial–mesenchymal transition via YAP and β-catenin pathway regulation |
| title_fullStr | Polygonum barbatum extract reduces colorectal cancer cell proliferation, migration, invasion, and epithelial–mesenchymal transition via YAP and β-catenin pathway regulation |
| title_full_unstemmed | Polygonum barbatum extract reduces colorectal cancer cell proliferation, migration, invasion, and epithelial–mesenchymal transition via YAP and β-catenin pathway regulation |
| title_short | Polygonum barbatum extract reduces colorectal cancer cell proliferation, migration, invasion, and epithelial–mesenchymal transition via YAP and β-catenin pathway regulation |
| title_sort | polygonum barbatum extract reduces colorectal cancer cell proliferation migration invasion and epithelial mesenchymal transition via yap and β catenin pathway regulation |
| url | https://doi.org/10.1038/s41598-023-45630-1 |
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