<i>Maackia amurensis</i> Rupr. et Maxim.: Supercritical CO<sub>2</sub> Extraction and Mass Spectrometric Characterization of Chemical Constituents
Three types of extraction were used to obtain biologically active substances from the heartwood of <i>M. amurensis</i>: supercritical CO<sub>2</sub> extraction, maceration with EtOH, and maceration with MeOH. The supercritical extraction method proved to be the most effective...
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MDPI AG
2023-02-01
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| Series: | Molecules |
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| author | Mayya P. Razgonova Elena I. Cherevach Lyudmila A. Tekutyeva Sergey A. Fedoreyev Natalia P. Mishchenko Darya V. Tarbeeva Ekaterina N. Demidova Nikita S. Kirilenko Kirill Golokhvast |
| author_facet | Mayya P. Razgonova Elena I. Cherevach Lyudmila A. Tekutyeva Sergey A. Fedoreyev Natalia P. Mishchenko Darya V. Tarbeeva Ekaterina N. Demidova Nikita S. Kirilenko Kirill Golokhvast |
| author_sort | Mayya P. Razgonova |
| collection | DOAJ |
| description | Three types of extraction were used to obtain biologically active substances from the heartwood of <i>M. amurensis</i>: supercritical CO<sub>2</sub> extraction, maceration with EtOH, and maceration with MeOH. The supercritical extraction method proved to be the most effective type of extraction, giving the highest yield of biologically active substances. Several experimental conditions were investigated in the pressure range of 50–400 bar, with 2% of ethanol as co-solvent in the liquid phase at a temperature in the range of 31–70 °C. The most effective extraction conditions are: pressure of 100 bar and a temperature of 55 °C for <i>M. amurensis</i> heartwood. The heartwood of <i>M. amurensis</i> contains various polyphenolic compounds and compounds of other chemical groups with valuable biological activity. Tandem mass spectrometry (HPLC-ESI—ion trap) was applied to detect target analytes. High-accuracy mass spectrometric data were recorded on an ion trap equipped with an ESI source in the modes of negative and positive ions. The four-stage ion separation mode was implemented. Sixty-six different biologically active components have been identified in <i>M. amurensis</i> extracts. Twenty-two polyphenols were identified for the first time in the genus <i>Maackia</i>. |
| first_indexed | 2024-03-11T07:17:22Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T07:17:22Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-23e8762c12d4495f9a1e1aeaec986cd42023-11-17T08:10:59ZengMDPI AGMolecules1420-30492023-02-01285202610.3390/molecules28052026<i>Maackia amurensis</i> Rupr. et Maxim.: Supercritical CO<sub>2</sub> Extraction and Mass Spectrometric Characterization of Chemical ConstituentsMayya P. Razgonova0Elena I. Cherevach1Lyudmila A. Tekutyeva2Sergey A. Fedoreyev3Natalia P. Mishchenko4Darya V. Tarbeeva5Ekaterina N. Demidova6Nikita S. Kirilenko7Kirill Golokhvast8N.I. Vavilov All-Russian Institute of Plant Genetic Resources, B. Morskaya 42-44, 190000 Saint Petersburg, RussiaDepartment of Pharmacy and Pharmacology, School of Biomedicine, Far Eastern Federal University, Sukhanova 8, 690950 Vladivostok, RussiaDepartment of Pharmacy and Pharmacology, School of Biomedicine, Far Eastern Federal University, Sukhanova 8, 690950 Vladivostok, RussiaDepartment of Pharmacy and Pharmacology, School of Biomedicine, Far Eastern Federal University, Sukhanova 8, 690950 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Science, Prospect 100 let Vladivostoku 159, 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Science, Prospect 100 let Vladivostoku 159, 690022 Vladivostok, RussiaDepartment of Pharmacy and Pharmacology, School of Biomedicine, Far Eastern Federal University, Sukhanova 8, 690950 Vladivostok, RussiaDepartment of Pharmacy and Pharmacology, School of Biomedicine, Far Eastern Federal University, Sukhanova 8, 690950 Vladivostok, RussiaDepartment of Pharmacy and Pharmacology, School of Biomedicine, Far Eastern Federal University, Sukhanova 8, 690950 Vladivostok, RussiaThree types of extraction were used to obtain biologically active substances from the heartwood of <i>M. amurensis</i>: supercritical CO<sub>2</sub> extraction, maceration with EtOH, and maceration with MeOH. The supercritical extraction method proved to be the most effective type of extraction, giving the highest yield of biologically active substances. Several experimental conditions were investigated in the pressure range of 50–400 bar, with 2% of ethanol as co-solvent in the liquid phase at a temperature in the range of 31–70 °C. The most effective extraction conditions are: pressure of 100 bar and a temperature of 55 °C for <i>M. amurensis</i> heartwood. The heartwood of <i>M. amurensis</i> contains various polyphenolic compounds and compounds of other chemical groups with valuable biological activity. Tandem mass spectrometry (HPLC-ESI—ion trap) was applied to detect target analytes. High-accuracy mass spectrometric data were recorded on an ion trap equipped with an ESI source in the modes of negative and positive ions. The four-stage ion separation mode was implemented. Sixty-six different biologically active components have been identified in <i>M. amurensis</i> extracts. Twenty-two polyphenols were identified for the first time in the genus <i>Maackia</i>.https://www.mdpi.com/1420-3049/28/5/2026<i>Maackia amurensis</i>CO<sub>2</sub> extractiontandem mass spectrometrypolyphenolsbioactive compounds |
| spellingShingle | Mayya P. Razgonova Elena I. Cherevach Lyudmila A. Tekutyeva Sergey A. Fedoreyev Natalia P. Mishchenko Darya V. Tarbeeva Ekaterina N. Demidova Nikita S. Kirilenko Kirill Golokhvast <i>Maackia amurensis</i> Rupr. et Maxim.: Supercritical CO<sub>2</sub> Extraction and Mass Spectrometric Characterization of Chemical Constituents Molecules <i>Maackia amurensis</i> CO<sub>2</sub> extraction tandem mass spectrometry polyphenols bioactive compounds |
| title | <i>Maackia amurensis</i> Rupr. et Maxim.: Supercritical CO<sub>2</sub> Extraction and Mass Spectrometric Characterization of Chemical Constituents |
| title_full | <i>Maackia amurensis</i> Rupr. et Maxim.: Supercritical CO<sub>2</sub> Extraction and Mass Spectrometric Characterization of Chemical Constituents |
| title_fullStr | <i>Maackia amurensis</i> Rupr. et Maxim.: Supercritical CO<sub>2</sub> Extraction and Mass Spectrometric Characterization of Chemical Constituents |
| title_full_unstemmed | <i>Maackia amurensis</i> Rupr. et Maxim.: Supercritical CO<sub>2</sub> Extraction and Mass Spectrometric Characterization of Chemical Constituents |
| title_short | <i>Maackia amurensis</i> Rupr. et Maxim.: Supercritical CO<sub>2</sub> Extraction and Mass Spectrometric Characterization of Chemical Constituents |
| title_sort | i maackia amurensis i rupr et maxim supercritical co sub 2 sub extraction and mass spectrometric characterization of chemical constituents |
| topic | <i>Maackia amurensis</i> CO<sub>2</sub> extraction tandem mass spectrometry polyphenols bioactive compounds |
| url | https://www.mdpi.com/1420-3049/28/5/2026 |
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