Temporal Expression of Myogenic Regulatory Genes in Different Chicken Breeds during Embryonic Development
The basic units of skeletal muscle in all vertebrates are multinucleate myofibers, which are formed from the fusion of mononuclear myoblasts during the embryonic period. In order to understand the regulation of embryonic muscle development, we selected four chicken breeds, namely, Cornish (CN), Whit...
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/17/10115 |
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| author | Shuang Gu Chaoliang Wen Junying Li Honghong Liu Qiang Huang Jiangxia Zheng Congjiao Sun Ning Yang |
| author_facet | Shuang Gu Chaoliang Wen Junying Li Honghong Liu Qiang Huang Jiangxia Zheng Congjiao Sun Ning Yang |
| author_sort | Shuang Gu |
| collection | DOAJ |
| description | The basic units of skeletal muscle in all vertebrates are multinucleate myofibers, which are formed from the fusion of mononuclear myoblasts during the embryonic period. In order to understand the regulation of embryonic muscle development, we selected four chicken breeds, namely, Cornish (CN), White Plymouth Rock (WPR), White Leghorn (WL), and Beijing-You Chicken (BYC), for evaluation of their temporal expression patterns of known key regulatory genes (<i>Myomaker</i>, <i>MYOD</i>, and <i>MSTN</i>) during pectoral muscle (PM) and thigh muscle (TM) development. The highest expression level of <i>Myomaker</i> occurred from embryonic days E13 to E15 for all breeds, indicating that it was the crucial stage of myoblast fusion. Interestingly, the fast-growing CN showed the highest gene expression level of <i>Myomaker</i> during the crucial stage. The <i>MYOD</i> gene expression at D1 was much higher, implying that <i>MYOD</i> might have an important role after hatching. Histomorphology of PM and TM suggested that the myofibers was largely complete at E17, which was speculated to have occurred because of the expression increase in <i>MSTN</i> and the expression decrease in <i>Myomaker</i>. Our research contributes to lay a foundation for the study of myofiber development during the embryonic period in different chicken breeds. |
| first_indexed | 2024-03-10T01:41:04Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T01:41:04Z |
| publishDate | 2022-09-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-23ea6e8c1e17479abf84cc7d4d553e052023-11-23T13:23:18ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123171011510.3390/ijms231710115Temporal Expression of Myogenic Regulatory Genes in Different Chicken Breeds during Embryonic DevelopmentShuang Gu0Chaoliang Wen1Junying Li2Honghong Liu3Qiang Huang4Jiangxia Zheng5Congjiao Sun6Ning Yang7National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100193, ChinaNational Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100193, ChinaNational Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100193, ChinaNational Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100193, ChinaNational Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100193, ChinaNational Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100193, ChinaNational Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100193, ChinaNational Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100193, ChinaThe basic units of skeletal muscle in all vertebrates are multinucleate myofibers, which are formed from the fusion of mononuclear myoblasts during the embryonic period. In order to understand the regulation of embryonic muscle development, we selected four chicken breeds, namely, Cornish (CN), White Plymouth Rock (WPR), White Leghorn (WL), and Beijing-You Chicken (BYC), for evaluation of their temporal expression patterns of known key regulatory genes (<i>Myomaker</i>, <i>MYOD</i>, and <i>MSTN</i>) during pectoral muscle (PM) and thigh muscle (TM) development. The highest expression level of <i>Myomaker</i> occurred from embryonic days E13 to E15 for all breeds, indicating that it was the crucial stage of myoblast fusion. Interestingly, the fast-growing CN showed the highest gene expression level of <i>Myomaker</i> during the crucial stage. The <i>MYOD</i> gene expression at D1 was much higher, implying that <i>MYOD</i> might have an important role after hatching. Histomorphology of PM and TM suggested that the myofibers was largely complete at E17, which was speculated to have occurred because of the expression increase in <i>MSTN</i> and the expression decrease in <i>Myomaker</i>. Our research contributes to lay a foundation for the study of myofiber development during the embryonic period in different chicken breeds.https://www.mdpi.com/1422-0067/23/17/10115chickenembryonic periodgene expressionmyoblast fusionmuscle development |
| spellingShingle | Shuang Gu Chaoliang Wen Junying Li Honghong Liu Qiang Huang Jiangxia Zheng Congjiao Sun Ning Yang Temporal Expression of Myogenic Regulatory Genes in Different Chicken Breeds during Embryonic Development International Journal of Molecular Sciences chicken embryonic period gene expression myoblast fusion muscle development |
| title | Temporal Expression of Myogenic Regulatory Genes in Different Chicken Breeds during Embryonic Development |
| title_full | Temporal Expression of Myogenic Regulatory Genes in Different Chicken Breeds during Embryonic Development |
| title_fullStr | Temporal Expression of Myogenic Regulatory Genes in Different Chicken Breeds during Embryonic Development |
| title_full_unstemmed | Temporal Expression of Myogenic Regulatory Genes in Different Chicken Breeds during Embryonic Development |
| title_short | Temporal Expression of Myogenic Regulatory Genes in Different Chicken Breeds during Embryonic Development |
| title_sort | temporal expression of myogenic regulatory genes in different chicken breeds during embryonic development |
| topic | chicken embryonic period gene expression myoblast fusion muscle development |
| url | https://www.mdpi.com/1422-0067/23/17/10115 |
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