Evolution of Complex Target SELEX to Identify Aptamers against Mammalian Cell-Surface Antigens
The demand has increased for sophisticated molecular tools with improved detection limits. Such molecules should be simple in structure, yet stable enough for clinical applications. Nucleic acid aptamers (NAAs) represent a class of molecules able to meet this demand. In particular, aptamers, a class...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-01-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/22/2/215 |
| _version_ | 1819156802625863680 |
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| author | Prabodhika Mallikaratchy |
| author_facet | Prabodhika Mallikaratchy |
| author_sort | Prabodhika Mallikaratchy |
| collection | DOAJ |
| description | The demand has increased for sophisticated molecular tools with improved detection limits. Such molecules should be simple in structure, yet stable enough for clinical applications. Nucleic acid aptamers (NAAs) represent a class of molecules able to meet this demand. In particular, aptamers, a class of small nucleic acid ligands that are composed of single-stranded modified/unmodified RNA/DNA molecules, can be evolved from a complex library using Systematic Evolution of Ligands by EXponential enrichment (SELEX) against almost any molecule. Since its introduction in 1990, in stages, SELEX technology has itself undergone several modifications, improving selection and broadening the repertoire of targets. This review summarizes these milestones that have pushed the field forward, allowing researchers to generate aptamers that can potentially be applied as therapeutic and diagnostic agents. |
| first_indexed | 2024-12-22T15:58:40Z |
| format | Article |
| id | doaj.art-23eac24456794a73a578f446ee865c13 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-22T15:58:40Z |
| publishDate | 2017-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-23eac24456794a73a578f446ee865c132022-12-21T18:20:44ZengMDPI AGMolecules1420-30492017-01-0122221510.3390/molecules22020215molecules22020215Evolution of Complex Target SELEX to Identify Aptamers against Mammalian Cell-Surface AntigensPrabodhika Mallikaratchy0Department of Chemistry, Lehman College, The City University of New York, 250 Bedford Park Blvd. West, Bronx, NY 10468, USAThe demand has increased for sophisticated molecular tools with improved detection limits. Such molecules should be simple in structure, yet stable enough for clinical applications. Nucleic acid aptamers (NAAs) represent a class of molecules able to meet this demand. In particular, aptamers, a class of small nucleic acid ligands that are composed of single-stranded modified/unmodified RNA/DNA molecules, can be evolved from a complex library using Systematic Evolution of Ligands by EXponential enrichment (SELEX) against almost any molecule. Since its introduction in 1990, in stages, SELEX technology has itself undergone several modifications, improving selection and broadening the repertoire of targets. This review summarizes these milestones that have pushed the field forward, allowing researchers to generate aptamers that can potentially be applied as therapeutic and diagnostic agents.http://www.mdpi.com/1420-3049/22/2/215SELEXnucleic acid aptamerscell-surface markers |
| spellingShingle | Prabodhika Mallikaratchy Evolution of Complex Target SELEX to Identify Aptamers against Mammalian Cell-Surface Antigens Molecules SELEX nucleic acid aptamers cell-surface markers |
| title | Evolution of Complex Target SELEX to Identify Aptamers against Mammalian Cell-Surface Antigens |
| title_full | Evolution of Complex Target SELEX to Identify Aptamers against Mammalian Cell-Surface Antigens |
| title_fullStr | Evolution of Complex Target SELEX to Identify Aptamers against Mammalian Cell-Surface Antigens |
| title_full_unstemmed | Evolution of Complex Target SELEX to Identify Aptamers against Mammalian Cell-Surface Antigens |
| title_short | Evolution of Complex Target SELEX to Identify Aptamers against Mammalian Cell-Surface Antigens |
| title_sort | evolution of complex target selex to identify aptamers against mammalian cell surface antigens |
| topic | SELEX nucleic acid aptamers cell-surface markers |
| url | http://www.mdpi.com/1420-3049/22/2/215 |
| work_keys_str_mv | AT prabodhikamallikaratchy evolutionofcomplextargetselextoidentifyaptamersagainstmammaliancellsurfaceantigens |