Effect of electroacupuncture on the mRNA and protein expression of Rho-A and Rho-associated kinase II in spinal cord injury rats
Electroacupuncture is beneficial for the recovery of spinal cord injury, but the underlying mechanism is unclear. The Rho/Rho-associated kinase (ROCK) signaling pathway regulates the actin cytoskeleton by controlling the adhesive and migratory behaviors of cells that could inhibit neurite regrowth a...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2017-01-01
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| Series: | Neural Regeneration Research |
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| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2017;volume=12;issue=2;spage=276;epage=282;aulast=Min |
| _version_ | 1828495530561372160 |
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| author | You-jiang Min Li-li-qiang Ding Li-hong Cheng Wei-ping Xiao Xing-wei He Hui Zhang Zhi-yun Min Jia Pei |
| author_facet | You-jiang Min Li-li-qiang Ding Li-hong Cheng Wei-ping Xiao Xing-wei He Hui Zhang Zhi-yun Min Jia Pei |
| author_sort | You-jiang Min |
| collection | DOAJ |
| description | Electroacupuncture is beneficial for the recovery of spinal cord injury, but the underlying mechanism is unclear. The Rho/Rho-associated kinase (ROCK) signaling pathway regulates the actin cytoskeleton by controlling the adhesive and migratory behaviors of cells that could inhibit neurite regrowth after neural injury and consequently hinder the recovery from spinal cord injury. Therefore, we hypothesized electroacupuncture could affect the Rho/ROCK signaling pathway to promote the recovery of spinal cord injury. In our experiments, the spinal cord injury in adult Sprague-Dawley rats was caused by an impact device. Those rats were subjected to electroacupuncture at Yaoyangguan (GV3), Dazhui (GV14), Zusanli (ST36) and Ciliao (BL32) and/or monosialoganglioside treatment. Behavioral scores revealed that the hindlimb motor functions improved with those treatments. Real-time quantitative polymerase chain reaction, fluorescence in situ hybridization and western blot assay showed that electroacupuncture suppressed the mRNA and protein expression of Rho-A and Rho-associated kinase II (ROCKII) of injured spinal cord. Although monosialoganglioside promoted the recovery of hindlimb motor function, monosialoganglioside did not affect the expression of Rho-A and ROCKII. However, electroacupuncture combined with monosialoganglioside did not further improve the motor function or suppress the expression of Rho-A and ROCKII. Our data suggested that the electroacupuncture could specifically inhibit the activation of the Rho/ROCK signaling pathway thus partially contributing to the repair of injured spinal cord. Monosialoganglioside could promote the motor function but did not suppress expression of RhoA and ROCKII. There was no synergistic effect of electroacupuncture combined with monosialoganglioside. |
| first_indexed | 2024-12-11T12:13:39Z |
| format | Article |
| id | doaj.art-23ed95d344624aef96b9a17c8f10ae3d |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-11T12:13:39Z |
| publishDate | 2017-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-23ed95d344624aef96b9a17c8f10ae3d2022-12-22T01:07:42ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742017-01-0112227628210.4103/1673-5374.200811Effect of electroacupuncture on the mRNA and protein expression of Rho-A and Rho-associated kinase II in spinal cord injury ratsYou-jiang MinLi-li-qiang DingLi-hong ChengWei-ping XiaoXing-wei HeHui ZhangZhi-yun MinJia PeiElectroacupuncture is beneficial for the recovery of spinal cord injury, but the underlying mechanism is unclear. The Rho/Rho-associated kinase (ROCK) signaling pathway regulates the actin cytoskeleton by controlling the adhesive and migratory behaviors of cells that could inhibit neurite regrowth after neural injury and consequently hinder the recovery from spinal cord injury. Therefore, we hypothesized electroacupuncture could affect the Rho/ROCK signaling pathway to promote the recovery of spinal cord injury. In our experiments, the spinal cord injury in adult Sprague-Dawley rats was caused by an impact device. Those rats were subjected to electroacupuncture at Yaoyangguan (GV3), Dazhui (GV14), Zusanli (ST36) and Ciliao (BL32) and/or monosialoganglioside treatment. Behavioral scores revealed that the hindlimb motor functions improved with those treatments. Real-time quantitative polymerase chain reaction, fluorescence in situ hybridization and western blot assay showed that electroacupuncture suppressed the mRNA and protein expression of Rho-A and Rho-associated kinase II (ROCKII) of injured spinal cord. Although monosialoganglioside promoted the recovery of hindlimb motor function, monosialoganglioside did not affect the expression of Rho-A and ROCKII. However, electroacupuncture combined with monosialoganglioside did not further improve the motor function or suppress the expression of Rho-A and ROCKII. Our data suggested that the electroacupuncture could specifically inhibit the activation of the Rho/ROCK signaling pathway thus partially contributing to the repair of injured spinal cord. Monosialoganglioside could promote the motor function but did not suppress expression of RhoA and ROCKII. There was no synergistic effect of electroacupuncture combined with monosialoganglioside.http://www.nrronline.org/article.asp?issn=1673-5374;year=2017;volume=12;issue=2;spage=276;epage=282;aulast=Minnerve regeneration; spinal cord injury; electroacupuncture; Rho/Rho-associated kinase signaling pathway; monosialoganglioside; motor function; cytoskeleton; real-time quantitative polymerase chain reaction; western blot assay; hybridization in situ; neural regeneration |
| spellingShingle | You-jiang Min Li-li-qiang Ding Li-hong Cheng Wei-ping Xiao Xing-wei He Hui Zhang Zhi-yun Min Jia Pei Effect of electroacupuncture on the mRNA and protein expression of Rho-A and Rho-associated kinase II in spinal cord injury rats Neural Regeneration Research nerve regeneration; spinal cord injury; electroacupuncture; Rho/Rho-associated kinase signaling pathway; monosialoganglioside; motor function; cytoskeleton; real-time quantitative polymerase chain reaction; western blot assay; hybridization in situ; neural regeneration |
| title | Effect of electroacupuncture on the mRNA and protein expression of Rho-A and Rho-associated kinase II in spinal cord injury rats |
| title_full | Effect of electroacupuncture on the mRNA and protein expression of Rho-A and Rho-associated kinase II in spinal cord injury rats |
| title_fullStr | Effect of electroacupuncture on the mRNA and protein expression of Rho-A and Rho-associated kinase II in spinal cord injury rats |
| title_full_unstemmed | Effect of electroacupuncture on the mRNA and protein expression of Rho-A and Rho-associated kinase II in spinal cord injury rats |
| title_short | Effect of electroacupuncture on the mRNA and protein expression of Rho-A and Rho-associated kinase II in spinal cord injury rats |
| title_sort | effect of electroacupuncture on the mrna and protein expression of rho a and rho associated kinase ii in spinal cord injury rats |
| topic | nerve regeneration; spinal cord injury; electroacupuncture; Rho/Rho-associated kinase signaling pathway; monosialoganglioside; motor function; cytoskeleton; real-time quantitative polymerase chain reaction; western blot assay; hybridization in situ; neural regeneration |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2017;volume=12;issue=2;spage=276;epage=282;aulast=Min |
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