Calcitonin Directly Inhibits Luteinizing Hormone-Stimulated Progesterone Production in Granulosa Cells of the Largest Follicle of Hen
The present study was performed to demonstrate the presence of receptors for avian calcitonin (cCT) in ovarian granulosa cells of the largest follicle of laying hens and to determine the effect of cCT on LH-stimulated P4 production. The cCT binding in the granulosa cells of the largest follicle was...
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2010-04-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/47/2/47_009084/_pdf/-char/en |
| _version_ | 1797816165124276224 |
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| author | Kaori Nakagawa-Mizuyachi Tetsuya Takahashi Shunsuke Kasai Hiroyuki Nakayama Mitsuo Kawashima |
| author_facet | Kaori Nakagawa-Mizuyachi Tetsuya Takahashi Shunsuke Kasai Hiroyuki Nakayama Mitsuo Kawashima |
| author_sort | Kaori Nakagawa-Mizuyachi |
| collection | DOAJ |
| description | The present study was performed to demonstrate the presence of receptors for avian calcitonin (cCT) in ovarian granulosa cells of the largest follicle of laying hens and to determine the effect of cCT on LH-stimulated P4 production. The cCT binding in the granulosa cells of the largest follicle was determined using a [125I]CT binding assay. The binding properties in the granulosa cells satisfied the criteria of a receptor-ligand interaction in terms of specificity, saturation, high affinity and limited capacity. During incubation of ovarian granulosa cells in vitro, cCT alone did not affect the P4 production alone, but cCT decreased the amount of LH-stimulated P4 production and the sensitivity to LH. The present study suggests that cCT may act directly on the granulosa cell of the largest follicle via its receptor binding and inhibit P4 production in response to LH in hens. |
| first_indexed | 2024-03-13T08:33:39Z |
| format | Article |
| id | doaj.art-23ef714ed16e4da2bda675db0905e881 |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:33:39Z |
| publishDate | 2010-04-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-23ef714ed16e4da2bda675db0905e8812023-05-31T00:53:25ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862010-04-0147217017510.2141/jpsa.009084jpsaCalcitonin Directly Inhibits Luteinizing Hormone-Stimulated Progesterone Production in Granulosa Cells of the Largest Follicle of HenKaori Nakagawa-Mizuyachi0Tetsuya Takahashi1Shunsuke Kasai2Hiroyuki Nakayama3Mitsuo Kawashima4The United Graduate School of Agricultural Science, Gifu University, JapanDepartment of Avian Endocrinology, Gifu University, JapanDepartment of Avian Endocrinology, Gifu University, JapanThe United Graduate School of Agricultural Science, Gifu University, JapanThe United Graduate School of Agricultural Science, Gifu University, JapanThe present study was performed to demonstrate the presence of receptors for avian calcitonin (cCT) in ovarian granulosa cells of the largest follicle of laying hens and to determine the effect of cCT on LH-stimulated P4 production. The cCT binding in the granulosa cells of the largest follicle was determined using a [125I]CT binding assay. The binding properties in the granulosa cells satisfied the criteria of a receptor-ligand interaction in terms of specificity, saturation, high affinity and limited capacity. During incubation of ovarian granulosa cells in vitro, cCT alone did not affect the P4 production alone, but cCT decreased the amount of LH-stimulated P4 production and the sensitivity to LH. The present study suggests that cCT may act directly on the granulosa cell of the largest follicle via its receptor binding and inhibit P4 production in response to LH in hens.https://www.jstage.jst.go.jp/article/jpsa/47/2/47_009084/_pdf/-char/encalcitoninhengraulosa cellovaryprogesterone |
| spellingShingle | Kaori Nakagawa-Mizuyachi Tetsuya Takahashi Shunsuke Kasai Hiroyuki Nakayama Mitsuo Kawashima Calcitonin Directly Inhibits Luteinizing Hormone-Stimulated Progesterone Production in Granulosa Cells of the Largest Follicle of Hen The Journal of Poultry Science calcitonin hen graulosa cell ovary progesterone |
| title | Calcitonin Directly Inhibits Luteinizing Hormone-Stimulated Progesterone Production in Granulosa Cells of the Largest Follicle of Hen |
| title_full | Calcitonin Directly Inhibits Luteinizing Hormone-Stimulated Progesterone Production in Granulosa Cells of the Largest Follicle of Hen |
| title_fullStr | Calcitonin Directly Inhibits Luteinizing Hormone-Stimulated Progesterone Production in Granulosa Cells of the Largest Follicle of Hen |
| title_full_unstemmed | Calcitonin Directly Inhibits Luteinizing Hormone-Stimulated Progesterone Production in Granulosa Cells of the Largest Follicle of Hen |
| title_short | Calcitonin Directly Inhibits Luteinizing Hormone-Stimulated Progesterone Production in Granulosa Cells of the Largest Follicle of Hen |
| title_sort | calcitonin directly inhibits luteinizing hormone stimulated progesterone production in granulosa cells of the largest follicle of hen |
| topic | calcitonin hen graulosa cell ovary progesterone |
| url | https://www.jstage.jst.go.jp/article/jpsa/47/2/47_009084/_pdf/-char/en |
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