Screening of <i>Lactiplantibacillus plantarum</i> 67 with Strong Adhesion to Caco-2 Cells and the Effects of Protective Agents on Its Adhesion Ability during Vacuum Freeze Drying
Adhesion to the intestinal tract provides the foundation for Lactobacillus to exert its benefits. Vacuum freeze-drying (VFD) is currently one of the main processing methods for Lactobacillus products. Therefore, the effects of VFD on the adhesion and survival of <i>Lactiplantibacillus plantaru...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/19/3604 |
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| author | Dawei Chen Congcong Guo Chenyu Ren Zihan Xia Haiyan Xu Hengxian Qu Yunchao Wa Chengran Guan Chenchen Zhang Jianya Qian Ruixia Gu |
| author_facet | Dawei Chen Congcong Guo Chenyu Ren Zihan Xia Haiyan Xu Hengxian Qu Yunchao Wa Chengran Guan Chenchen Zhang Jianya Qian Ruixia Gu |
| author_sort | Dawei Chen |
| collection | DOAJ |
| description | Adhesion to the intestinal tract provides the foundation for Lactobacillus to exert its benefits. Vacuum freeze-drying (VFD) is currently one of the main processing methods for Lactobacillus products. Therefore, the effects of VFD on the adhesion and survival of <i>Lactiplantibacillus plantarum</i> 67 were investigated in this study. The results show that <i>L. plantarum</i> 67 exhibits remarkable tolerance following successive exposure to simulated saliva, gastric juice and intestinal juice, and also has a strong adhesion ability to Caco-2 cells. The adhesion and survival rates of <i>L. plantarum</i> 67 significantly decreased after VFD in phosphate-buffered saline (PBS), whereas they significantly increased in protective agents (PAs) (<i>p</i> < 0.05). Scanning electron microscope observations show that <i>L. plantarum</i> 67 aggregated more to Caco-2 cells in PAs than in PBS, and its shape and size were protected. Proteomics detection findings indicated that differentially expressed proteins (DEPs) related to adhesins and vitality and their pathways in <i>L. plantarum</i> 67 were significantly affected by VFD (<i>p</i> < 0.05). However, the expression of DEPs (such as cold shock protein, cell surface protein, adherence protein, chitin-binding domain and extracellular transglycosylase, membrane-bound protein) was improved by PAs. Compared with PBS, the PAs significantly adjusted the phosphotransferase system and amino sugar and nucleotide sugar metabolism pathways (<i>p</i> < 0.05). VFD decreased the adhesion and vitality of <i>L. plantarum</i> 67, while the PAs could exert protective effects by regulating proteins and pathways related to adhesion and vitality. |
| first_indexed | 2024-03-10T21:44:23Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T21:44:23Z |
| publishDate | 2023-09-01 |
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| record_format | Article |
| series | Foods |
| spelling | doaj.art-23efc4c1472c41599423cd7e5c022ea02023-11-19T14:23:12ZengMDPI AGFoods2304-81582023-09-011219360410.3390/foods12193604Screening of <i>Lactiplantibacillus plantarum</i> 67 with Strong Adhesion to Caco-2 Cells and the Effects of Protective Agents on Its Adhesion Ability during Vacuum Freeze DryingDawei Chen0Congcong Guo1Chenyu Ren2Zihan Xia3Haiyan Xu4Hengxian Qu5Yunchao Wa6Chengran Guan7Chenchen Zhang8Jianya Qian9Ruixia Gu10College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaAdhesion to the intestinal tract provides the foundation for Lactobacillus to exert its benefits. Vacuum freeze-drying (VFD) is currently one of the main processing methods for Lactobacillus products. Therefore, the effects of VFD on the adhesion and survival of <i>Lactiplantibacillus plantarum</i> 67 were investigated in this study. The results show that <i>L. plantarum</i> 67 exhibits remarkable tolerance following successive exposure to simulated saliva, gastric juice and intestinal juice, and also has a strong adhesion ability to Caco-2 cells. The adhesion and survival rates of <i>L. plantarum</i> 67 significantly decreased after VFD in phosphate-buffered saline (PBS), whereas they significantly increased in protective agents (PAs) (<i>p</i> < 0.05). Scanning electron microscope observations show that <i>L. plantarum</i> 67 aggregated more to Caco-2 cells in PAs than in PBS, and its shape and size were protected. Proteomics detection findings indicated that differentially expressed proteins (DEPs) related to adhesins and vitality and their pathways in <i>L. plantarum</i> 67 were significantly affected by VFD (<i>p</i> < 0.05). However, the expression of DEPs (such as cold shock protein, cell surface protein, adherence protein, chitin-binding domain and extracellular transglycosylase, membrane-bound protein) was improved by PAs. Compared with PBS, the PAs significantly adjusted the phosphotransferase system and amino sugar and nucleotide sugar metabolism pathways (<i>p</i> < 0.05). VFD decreased the adhesion and vitality of <i>L. plantarum</i> 67, while the PAs could exert protective effects by regulating proteins and pathways related to adhesion and vitality.https://www.mdpi.com/2304-8158/12/19/3604vacuum freeze-dryingprotective agents<i>Lactiplantibacillus plantarum</i> 67adhesion |
| spellingShingle | Dawei Chen Congcong Guo Chenyu Ren Zihan Xia Haiyan Xu Hengxian Qu Yunchao Wa Chengran Guan Chenchen Zhang Jianya Qian Ruixia Gu Screening of <i>Lactiplantibacillus plantarum</i> 67 with Strong Adhesion to Caco-2 Cells and the Effects of Protective Agents on Its Adhesion Ability during Vacuum Freeze Drying Foods vacuum freeze-drying protective agents <i>Lactiplantibacillus plantarum</i> 67 adhesion |
| title | Screening of <i>Lactiplantibacillus plantarum</i> 67 with Strong Adhesion to Caco-2 Cells and the Effects of Protective Agents on Its Adhesion Ability during Vacuum Freeze Drying |
| title_full | Screening of <i>Lactiplantibacillus plantarum</i> 67 with Strong Adhesion to Caco-2 Cells and the Effects of Protective Agents on Its Adhesion Ability during Vacuum Freeze Drying |
| title_fullStr | Screening of <i>Lactiplantibacillus plantarum</i> 67 with Strong Adhesion to Caco-2 Cells and the Effects of Protective Agents on Its Adhesion Ability during Vacuum Freeze Drying |
| title_full_unstemmed | Screening of <i>Lactiplantibacillus plantarum</i> 67 with Strong Adhesion to Caco-2 Cells and the Effects of Protective Agents on Its Adhesion Ability during Vacuum Freeze Drying |
| title_short | Screening of <i>Lactiplantibacillus plantarum</i> 67 with Strong Adhesion to Caco-2 Cells and the Effects of Protective Agents on Its Adhesion Ability during Vacuum Freeze Drying |
| title_sort | screening of i lactiplantibacillus plantarum i 67 with strong adhesion to caco 2 cells and the effects of protective agents on its adhesion ability during vacuum freeze drying |
| topic | vacuum freeze-drying protective agents <i>Lactiplantibacillus plantarum</i> 67 adhesion |
| url | https://www.mdpi.com/2304-8158/12/19/3604 |
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