Changes in Light Energy Utilization in Photosystem II and Reactive Oxygen Species Generation in Potato Leaves by the Pinworm <i>Tuta absoluta</i>
We evaluated photosystem II (PSII) functionality in potato plants (<i>Solanum tuberosum</i> L.) before and after a 15 min feeding by the leaf miner <i>Tuta absoluta</i> using chlorophyll <i>a</i> fluorescence imaging analysis combined with reactive oxygen species...
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MDPI AG
2021-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/10/2984 |
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| author | Ilektra Sperdouli Stefanos Andreadis Julietta Moustaka Emmanuel Panteris Aphrodite Tsaballa Michael Moustakas |
| author_facet | Ilektra Sperdouli Stefanos Andreadis Julietta Moustaka Emmanuel Panteris Aphrodite Tsaballa Michael Moustakas |
| author_sort | Ilektra Sperdouli |
| collection | DOAJ |
| description | We evaluated photosystem II (PSII) functionality in potato plants (<i>Solanum tuberosum</i> L.) before and after a 15 min feeding by the leaf miner <i>Tuta absoluta</i> using chlorophyll <i>a</i> fluorescence imaging analysis combined with reactive oxygen species (ROS) detection. Fifteen minutes after feeding, we observed at the feeding zone and at the whole leaf a decrease in the effective quantum yield of photosystem II (PSII) photochemistry (Φ<i><sub>PSII</sub></i>). While at the feeding zone the quantum yield of regulated non-photochemical energy loss in PSII (Φ<i><sub>NPQ</sub></i>) did not change, at the whole leaf level there was a significant increase. As a result, at the feeding zone a significant increase in the quantum yield of non-regulated energy loss in PSII (Φ<i><sub>NO</sub></i>) occurred, but there was no change at the whole leaf level compared to that before feeding, indicating no change in singlet oxygen (<sup>1</sup>O<sub>2</sub>) formation. The decreased Φ<i><sub>PSII</sub></i> after feeding was due to a decreased fraction of open reaction centers (q<i><sub>p</sub></i>), since the efficiency of open PSII reaction centers to utilize the light energy (F<i>v</i>′/F<i>m</i>′) did not differ before and after feeding. The decreased fraction of open reaction centers resulted in increased excess excitation energy (EXC) at the feeding zone and at the whole leaf level, while hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) production was detected only at the feeding zone. Although the whole leaf PSII efficiency decreased compared to that before feeding, the maximum efficiency of PSII photochemistry (F<i>v</i>/F<i>m</i>), and the efficiency of the water-splitting complex on the donor side of PSII (F<i>v</i>/F<i>o</i>), did not differ to that before feeding, thus they cannot be considered as sensitive parameters to monitor biotic stress effects. Chlorophyll fluorescence imaging analysis proved to be a good indicator to monitor even short-term impacts of insect herbivory on photosynthetic function, and among the studied parameters, the reduction status of the plastoquinone pool (q<i><sub>p</sub></i>) was the most sensitive and suitable indicator to probe photosynthetic function under biotic stress. |
| first_indexed | 2024-03-10T11:18:49Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T11:18:49Z |
| publishDate | 2021-05-01 |
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| spelling | doaj.art-23f8561222704f93a6134cbfbda877492023-11-21T20:10:05ZengMDPI AGMolecules1420-30492021-05-012610298410.3390/molecules26102984Changes in Light Energy Utilization in Photosystem II and Reactive Oxygen Species Generation in Potato Leaves by the Pinworm <i>Tuta absoluta</i>Ilektra Sperdouli0Stefanos Andreadis1Julietta Moustaka2Emmanuel Panteris3Aphrodite Tsaballa4Michael Moustakas5Institute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organisation–Demeter (ELGO-Dimitra), 57001 Thermi, Thessaloniki, GreeceInstitute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organisation–Demeter (ELGO-Dimitra), 57001 Thermi, Thessaloniki, GreeceDepartment of Botany, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceDepartment of Botany, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceInstitute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organisation–Demeter (ELGO-Dimitra), 57001 Thermi, Thessaloniki, GreeceDepartment of Botany, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceWe evaluated photosystem II (PSII) functionality in potato plants (<i>Solanum tuberosum</i> L.) before and after a 15 min feeding by the leaf miner <i>Tuta absoluta</i> using chlorophyll <i>a</i> fluorescence imaging analysis combined with reactive oxygen species (ROS) detection. Fifteen minutes after feeding, we observed at the feeding zone and at the whole leaf a decrease in the effective quantum yield of photosystem II (PSII) photochemistry (Φ<i><sub>PSII</sub></i>). While at the feeding zone the quantum yield of regulated non-photochemical energy loss in PSII (Φ<i><sub>NPQ</sub></i>) did not change, at the whole leaf level there was a significant increase. As a result, at the feeding zone a significant increase in the quantum yield of non-regulated energy loss in PSII (Φ<i><sub>NO</sub></i>) occurred, but there was no change at the whole leaf level compared to that before feeding, indicating no change in singlet oxygen (<sup>1</sup>O<sub>2</sub>) formation. The decreased Φ<i><sub>PSII</sub></i> after feeding was due to a decreased fraction of open reaction centers (q<i><sub>p</sub></i>), since the efficiency of open PSII reaction centers to utilize the light energy (F<i>v</i>′/F<i>m</i>′) did not differ before and after feeding. The decreased fraction of open reaction centers resulted in increased excess excitation energy (EXC) at the feeding zone and at the whole leaf level, while hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) production was detected only at the feeding zone. Although the whole leaf PSII efficiency decreased compared to that before feeding, the maximum efficiency of PSII photochemistry (F<i>v</i>/F<i>m</i>), and the efficiency of the water-splitting complex on the donor side of PSII (F<i>v</i>/F<i>o</i>), did not differ to that before feeding, thus they cannot be considered as sensitive parameters to monitor biotic stress effects. Chlorophyll fluorescence imaging analysis proved to be a good indicator to monitor even short-term impacts of insect herbivory on photosynthetic function, and among the studied parameters, the reduction status of the plastoquinone pool (q<i><sub>p</sub></i>) was the most sensitive and suitable indicator to probe photosynthetic function under biotic stress.https://www.mdpi.com/1420-3049/26/10/2984biotic stresschlorophyll fluorescence imagingherbivore insectshydrogen peroxidelight energy usenon-photochemical quenching |
| spellingShingle | Ilektra Sperdouli Stefanos Andreadis Julietta Moustaka Emmanuel Panteris Aphrodite Tsaballa Michael Moustakas Changes in Light Energy Utilization in Photosystem II and Reactive Oxygen Species Generation in Potato Leaves by the Pinworm <i>Tuta absoluta</i> Molecules biotic stress chlorophyll fluorescence imaging herbivore insects hydrogen peroxide light energy use non-photochemical quenching |
| title | Changes in Light Energy Utilization in Photosystem II and Reactive Oxygen Species Generation in Potato Leaves by the Pinworm <i>Tuta absoluta</i> |
| title_full | Changes in Light Energy Utilization in Photosystem II and Reactive Oxygen Species Generation in Potato Leaves by the Pinworm <i>Tuta absoluta</i> |
| title_fullStr | Changes in Light Energy Utilization in Photosystem II and Reactive Oxygen Species Generation in Potato Leaves by the Pinworm <i>Tuta absoluta</i> |
| title_full_unstemmed | Changes in Light Energy Utilization in Photosystem II and Reactive Oxygen Species Generation in Potato Leaves by the Pinworm <i>Tuta absoluta</i> |
| title_short | Changes in Light Energy Utilization in Photosystem II and Reactive Oxygen Species Generation in Potato Leaves by the Pinworm <i>Tuta absoluta</i> |
| title_sort | changes in light energy utilization in photosystem ii and reactive oxygen species generation in potato leaves by the pinworm i tuta absoluta i |
| topic | biotic stress chlorophyll fluorescence imaging herbivore insects hydrogen peroxide light energy use non-photochemical quenching |
| url | https://www.mdpi.com/1420-3049/26/10/2984 |
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