Role of a GntR-family response regulator LbrA in Listeria monocytogenes biofilm formation.
The formation of Listeria monocytogenes biofilms contributes to persistent contamination in food processing facilities. A microarray comparison of L. monocytogenes between the transcriptome of the strong biofilm forming strain (Bfm(s)) Scott A and the weak biofilm forming (Bfm(w)) strain F2365 was c...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3720924?pdf=render |
| _version_ | 1828887631722708992 |
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| author | Andrew Wassinger Lu Zhang Erin Tracy Robert S Munson Sophia Kathariou Hua H Wang |
| author_facet | Andrew Wassinger Lu Zhang Erin Tracy Robert S Munson Sophia Kathariou Hua H Wang |
| author_sort | Andrew Wassinger |
| collection | DOAJ |
| description | The formation of Listeria monocytogenes biofilms contributes to persistent contamination in food processing facilities. A microarray comparison of L. monocytogenes between the transcriptome of the strong biofilm forming strain (Bfm(s)) Scott A and the weak biofilm forming (Bfm(w)) strain F2365 was conducted to identify genes potentially involved in biofilm formation. Among 951 genes with significant difference in expression between the two strains, a GntR-family response regulator encoding gene (LMOf2365_0414), designated lbrA, was found to be highly expressed in Scott A relative to F2365. A Scott A lbrA-deletion mutant, designated AW3, formed biofilm to a much lesser extent as compared to the parent strain by a rapid attachment assay and scanning electron microscopy. Complementation with lbrA from Scott A restored the Bfm(s) phenotype in the AW3 derivative. A second microarray assessment using the lbrA deletion mutant AW3 and the wild type Scott A revealed a total of 304 genes with expression significantly different between the two strains, indicating the potential regulatory role of LbrA in L. monocytogenes. A cloned copy of Scott A lbrA was unable to confer enhanced biofilm forming potential in F2365, suggesting that additional factors contributed to weak biofilm formation by F2365. |
| first_indexed | 2024-12-13T12:07:58Z |
| format | Article |
| id | doaj.art-23f8c25c926f47c18b0c2e9dbf7afce7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T12:07:58Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-23f8c25c926f47c18b0c2e9dbf7afce72022-12-21T23:46:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0187e7044810.1371/journal.pone.0070448Role of a GntR-family response regulator LbrA in Listeria monocytogenes biofilm formation.Andrew WassingerLu ZhangErin TracyRobert S MunsonSophia KathariouHua H WangThe formation of Listeria monocytogenes biofilms contributes to persistent contamination in food processing facilities. A microarray comparison of L. monocytogenes between the transcriptome of the strong biofilm forming strain (Bfm(s)) Scott A and the weak biofilm forming (Bfm(w)) strain F2365 was conducted to identify genes potentially involved in biofilm formation. Among 951 genes with significant difference in expression between the two strains, a GntR-family response regulator encoding gene (LMOf2365_0414), designated lbrA, was found to be highly expressed in Scott A relative to F2365. A Scott A lbrA-deletion mutant, designated AW3, formed biofilm to a much lesser extent as compared to the parent strain by a rapid attachment assay and scanning electron microscopy. Complementation with lbrA from Scott A restored the Bfm(s) phenotype in the AW3 derivative. A second microarray assessment using the lbrA deletion mutant AW3 and the wild type Scott A revealed a total of 304 genes with expression significantly different between the two strains, indicating the potential regulatory role of LbrA in L. monocytogenes. A cloned copy of Scott A lbrA was unable to confer enhanced biofilm forming potential in F2365, suggesting that additional factors contributed to weak biofilm formation by F2365.http://europepmc.org/articles/PMC3720924?pdf=render |
| spellingShingle | Andrew Wassinger Lu Zhang Erin Tracy Robert S Munson Sophia Kathariou Hua H Wang Role of a GntR-family response regulator LbrA in Listeria monocytogenes biofilm formation. PLoS ONE |
| title | Role of a GntR-family response regulator LbrA in Listeria monocytogenes biofilm formation. |
| title_full | Role of a GntR-family response regulator LbrA in Listeria monocytogenes biofilm formation. |
| title_fullStr | Role of a GntR-family response regulator LbrA in Listeria monocytogenes biofilm formation. |
| title_full_unstemmed | Role of a GntR-family response regulator LbrA in Listeria monocytogenes biofilm formation. |
| title_short | Role of a GntR-family response regulator LbrA in Listeria monocytogenes biofilm formation. |
| title_sort | role of a gntr family response regulator lbra in listeria monocytogenes biofilm formation |
| url | http://europepmc.org/articles/PMC3720924?pdf=render |
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