Detailed protocol for germ-free Drosophila melanogaster colonization with Propionibacterium spp. biofilms
Summary: In this protocol, we describe a germ-free Drosophila melanogaster model to investigate anaerobic bacterial biofilms. We detail how to establish Propionibacterium spp. biofilms in the fruit fly’s gut using an easy to carry out method.For complete details on the use and execution of this prot...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-06-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722002222 |
| _version_ | 1818141917173514240 |
|---|---|
| author | Vicky Bronnec Oleg A. Alexeyev |
| author_facet | Vicky Bronnec Oleg A. Alexeyev |
| author_sort | Vicky Bronnec |
| collection | DOAJ |
| description | Summary: In this protocol, we describe a germ-free Drosophila melanogaster model to investigate anaerobic bacterial biofilms. We detail how to establish Propionibacterium spp. biofilms in the fruit fly’s gut using an easy to carry out method.For complete details on the use and execution of this protocol, please refer to Bronnec and Alexeyev (2021) and Bronnec et al. (2022). |
| first_indexed | 2024-12-11T11:07:29Z |
| format | Article |
| id | doaj.art-23f9c25c4b024a90a217199de966c964 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-11T11:07:29Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-23f9c25c4b024a90a217199de966c9642022-12-22T01:09:39ZengElsevierSTAR Protocols2666-16672022-06-0132101342Detailed protocol for germ-free Drosophila melanogaster colonization with Propionibacterium spp. biofilmsVicky Bronnec0Oleg A. Alexeyev1Department of Medical Biosciences/Pathology, Umeå University, 90 187 Umeå, Sweden; Corresponding authorDepartment of Medical Biosciences/Pathology, Umeå University, 90 187 Umeå, Sweden; Corresponding authorSummary: In this protocol, we describe a germ-free Drosophila melanogaster model to investigate anaerobic bacterial biofilms. We detail how to establish Propionibacterium spp. biofilms in the fruit fly’s gut using an easy to carry out method.For complete details on the use and execution of this protocol, please refer to Bronnec and Alexeyev (2021) and Bronnec et al. (2022).http://www.sciencedirect.com/science/article/pii/S2666166722002222Health SciencesMicrobiologyMicroscopyModel Organisms |
| spellingShingle | Vicky Bronnec Oleg A. Alexeyev Detailed protocol for germ-free Drosophila melanogaster colonization with Propionibacterium spp. biofilms STAR Protocols Health Sciences Microbiology Microscopy Model Organisms |
| title | Detailed protocol for germ-free Drosophila melanogaster colonization with Propionibacterium spp. biofilms |
| title_full | Detailed protocol for germ-free Drosophila melanogaster colonization with Propionibacterium spp. biofilms |
| title_fullStr | Detailed protocol for germ-free Drosophila melanogaster colonization with Propionibacterium spp. biofilms |
| title_full_unstemmed | Detailed protocol for germ-free Drosophila melanogaster colonization with Propionibacterium spp. biofilms |
| title_short | Detailed protocol for germ-free Drosophila melanogaster colonization with Propionibacterium spp. biofilms |
| title_sort | detailed protocol for germ free drosophila melanogaster colonization with propionibacterium spp biofilms |
| topic | Health Sciences Microbiology Microscopy Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722002222 |
| work_keys_str_mv | AT vickybronnec detailedprotocolforgermfreedrosophilamelanogastercolonizationwithpropionibacteriumsppbiofilms AT olegaalexeyev detailedprotocolforgermfreedrosophilamelanogastercolonizationwithpropionibacteriumsppbiofilms |