Prevention of Loperamide-Induced Constipation in Mice and Alteration of 5-Hydroxytryotamine Signaling by <i>Ligilactobacillus salivarius</i> Li01
Although <i>Ligilactobacillus salivarius</i> Li01 (Li01) has shown much promise in preventing multiple gastrointestinal diseases, the potential of the probiotic in alleviating constipation and the related mechanisms remain unclear. In this study, the effects of Li01 were evaluated in a l...
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MDPI AG
2022-10-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/19/4083 |
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| author | Bo Qiu Lian Zhu Shuobo Zhang Shengyi Han Yiqiu Fei Furong Ba Björn Berglund Lanjuan Li Mingfei Yao |
| author_facet | Bo Qiu Lian Zhu Shuobo Zhang Shengyi Han Yiqiu Fei Furong Ba Björn Berglund Lanjuan Li Mingfei Yao |
| author_sort | Bo Qiu |
| collection | DOAJ |
| description | Although <i>Ligilactobacillus salivarius</i> Li01 (Li01) has shown much promise in preventing multiple gastrointestinal diseases, the potential of the probiotic in alleviating constipation and the related mechanisms remain unclear. In this study, the effects of Li01 were evaluated in a loperamide-induced constipation mouse model. The results demonstrated that Li01 intervention can relieve constipation symptoms by improving water content, quantity, and morphology of feces and act as an intestinal barrier structure protector. Furthermore, Li01 can modulate gut motility (gastrointestinal transit rate), the fluid transit-associated expression of aquaporins, and the serum parameters vasoactive intestinal peptide, substance P, and somatostatin. Constipation significantly increased the levels of 5-hydroxytryotamine (5-HT) in serum (<i>p</i> < 0.01) and decreased the levels in the intestine (<i>p</i> < 0.001). Due to its function of elevating the expression of tryptophan hydroxylase 1, this was reversed after Li01 treatment. Li01 also promoted the expression of 5-HT receptor 3 and 4, indicating that the 5-HT signaling pathway may play a critical role in the mechanism by which Li01 alleviate constipation symptoms. Additionally, Li01 significantly altered the gut microbiota composition by enhancing the ratio of <i>Firmicutes</i>/<i>Bacteroidetes</i> and increasing the abundance of <i>Rikenellaceae_RC9</i> genera. Based on the above results, Li01 may have the potential to effectively alleviate constipation by regulating the 5-HT pathway and alteration of the gut microbiota. |
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| language | English |
| last_indexed | 2024-03-09T21:19:32Z |
| publishDate | 2022-10-01 |
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| series | Nutrients |
| spelling | doaj.art-23fd718eea304a41b49a0962e0c80fe12023-11-23T21:25:21ZengMDPI AGNutrients2072-66432022-10-011419408310.3390/nu14194083Prevention of Loperamide-Induced Constipation in Mice and Alteration of 5-Hydroxytryotamine Signaling by <i>Ligilactobacillus salivarius</i> Li01Bo Qiu0Lian Zhu1Shuobo Zhang2Shengyi Han3Yiqiu Fei4Furong Ba5Björn Berglund6Lanjuan Li7Mingfei Yao8State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, ChinaSchool of Basic Medical Sciences and Forensic Medicine, Hangzhou Medical College, Hangzhou 310053, ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, ChinaDepartment of Biomedical and Clinical Sciences, Linköping University, SE-58183 Linköping, SwedenState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, ChinaAlthough <i>Ligilactobacillus salivarius</i> Li01 (Li01) has shown much promise in preventing multiple gastrointestinal diseases, the potential of the probiotic in alleviating constipation and the related mechanisms remain unclear. In this study, the effects of Li01 were evaluated in a loperamide-induced constipation mouse model. The results demonstrated that Li01 intervention can relieve constipation symptoms by improving water content, quantity, and morphology of feces and act as an intestinal barrier structure protector. Furthermore, Li01 can modulate gut motility (gastrointestinal transit rate), the fluid transit-associated expression of aquaporins, and the serum parameters vasoactive intestinal peptide, substance P, and somatostatin. Constipation significantly increased the levels of 5-hydroxytryotamine (5-HT) in serum (<i>p</i> < 0.01) and decreased the levels in the intestine (<i>p</i> < 0.001). Due to its function of elevating the expression of tryptophan hydroxylase 1, this was reversed after Li01 treatment. Li01 also promoted the expression of 5-HT receptor 3 and 4, indicating that the 5-HT signaling pathway may play a critical role in the mechanism by which Li01 alleviate constipation symptoms. Additionally, Li01 significantly altered the gut microbiota composition by enhancing the ratio of <i>Firmicutes</i>/<i>Bacteroidetes</i> and increasing the abundance of <i>Rikenellaceae_RC9</i> genera. Based on the above results, Li01 may have the potential to effectively alleviate constipation by regulating the 5-HT pathway and alteration of the gut microbiota.https://www.mdpi.com/2072-6643/14/19/4083constipation<i>Ligilactobacillus salivarius</i> Li015-hydroxytryotaminegut microbiota |
| spellingShingle | Bo Qiu Lian Zhu Shuobo Zhang Shengyi Han Yiqiu Fei Furong Ba Björn Berglund Lanjuan Li Mingfei Yao Prevention of Loperamide-Induced Constipation in Mice and Alteration of 5-Hydroxytryotamine Signaling by <i>Ligilactobacillus salivarius</i> Li01 Nutrients constipation <i>Ligilactobacillus salivarius</i> Li01 5-hydroxytryotamine gut microbiota |
| title | Prevention of Loperamide-Induced Constipation in Mice and Alteration of 5-Hydroxytryotamine Signaling by <i>Ligilactobacillus salivarius</i> Li01 |
| title_full | Prevention of Loperamide-Induced Constipation in Mice and Alteration of 5-Hydroxytryotamine Signaling by <i>Ligilactobacillus salivarius</i> Li01 |
| title_fullStr | Prevention of Loperamide-Induced Constipation in Mice and Alteration of 5-Hydroxytryotamine Signaling by <i>Ligilactobacillus salivarius</i> Li01 |
| title_full_unstemmed | Prevention of Loperamide-Induced Constipation in Mice and Alteration of 5-Hydroxytryotamine Signaling by <i>Ligilactobacillus salivarius</i> Li01 |
| title_short | Prevention of Loperamide-Induced Constipation in Mice and Alteration of 5-Hydroxytryotamine Signaling by <i>Ligilactobacillus salivarius</i> Li01 |
| title_sort | prevention of loperamide induced constipation in mice and alteration of 5 hydroxytryotamine signaling by i ligilactobacillus salivarius i li01 |
| topic | constipation <i>Ligilactobacillus salivarius</i> Li01 5-hydroxytryotamine gut microbiota |
| url | https://www.mdpi.com/2072-6643/14/19/4083 |
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