Evaluation of Streptococcus species isolated from subclinical sheep mastitis by molecular methods and determination of virulence factors and antimicrobial resistance genes
Streptococcus (S.) species are important pathogens that cause mastitis in sheep. The study aimed to examine Streptococcus species in sheep milk with subclinical mastitis, assessing their prevalence, antimicrobial resistance, and virulence genes. A total of 200 milk samples were collected from sheep...
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| Format: | Article |
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Czech Academy of Agricultural Sciences
2023-09-01
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| Series: | Veterinární Medicína |
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| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-202309-0002_evaluation-of-streptococcus-species-isolated-from-subclinical-sheep-mastitis-by-molecular-methods-and-determina.php |
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| author | V Ozavci HT Yuksel Dolgun S Kirkan Y Seferoglu Z Semen U Parin |
| author_facet | V Ozavci HT Yuksel Dolgun S Kirkan Y Seferoglu Z Semen U Parin |
| author_sort | V Ozavci |
| collection | DOAJ |
| description | Streptococcus (S.) species are important pathogens that cause mastitis in sheep. The study aimed to examine Streptococcus species in sheep milk with subclinical mastitis, assessing their prevalence, antimicrobial resistance, and virulence genes. A total of 200 milk samples were collected from sheep farms in İzmir's five districts. Out of 32 (28.6%) Streptococcus isolates identified by phenotypic methods, 25 were genotypically identified as S. uberis, 5 as S. agalactiae, and 2 as S. dysgalactiae. Disk diffusion was used to determine the antimicrobial resistance of the isolates. PCR was employed to identify antimicrobial resistance and virulence genes in the isolates. The highest resistance was found for cloxacillin (100%), and the highest sensitivity was found for florfenicol (84%). The most common resistance gene combination was tetM+tetS (3/32) for S. uberis in 9.4%. A total of five virulence genes were detected. GapC+sua (56.2%) constituted the most common gene pattern. The highest virulence gene gapC was detected in 78.1% (25/32) of the isolates. The cylE gene was not detected (0%) in the isolates. Streptococcus species may play a role in mastitis in sheep, emphasising the need for meticulous hygienic milking practices. |
| first_indexed | 2024-03-08T09:26:40Z |
| format | Article |
| id | doaj.art-23fdf8aae4284d3987afaefdf1fb4b74 |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-03-08T09:26:40Z |
| publishDate | 2023-09-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-23fdf8aae4284d3987afaefdf1fb4b742024-01-31T08:27:39ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922023-09-0168935936710.17221/42/2023-VETMEDvet-202309-0002Evaluation of Streptococcus species isolated from subclinical sheep mastitis by molecular methods and determination of virulence factors and antimicrobial resistance genesV Ozavci0HT Yuksel Dolgun1S Kirkan2Y Seferoglu3Z Semen4U Parin5Department of Microbiology, Faculty of Veterinary Medicine, Dokuz Eylul University, Izmir, TurkiyeDepartment of Microbiology, Faculty of Veterinary Medicine, Aydin Adnan Menderes University, Aydin, TurkiyeDepartment of Microbiology, Faculty of Veterinary Medicine, Aydin Adnan Menderes University, Aydin, TurkiyeDepartment of Microbiology, Faculty of Veterinary Medicine, Aydin Adnan Menderes University, Aydin, TurkiyeDepartment of Biochemistry, Faculty of Veterinary Medicine, Dokuz Eylul University, Izmir, TurkiyeDepartment of Microbiology, Faculty of Veterinary Medicine, Aydin Adnan Menderes University, Aydin, TurkiyeStreptococcus (S.) species are important pathogens that cause mastitis in sheep. The study aimed to examine Streptococcus species in sheep milk with subclinical mastitis, assessing their prevalence, antimicrobial resistance, and virulence genes. A total of 200 milk samples were collected from sheep farms in İzmir's five districts. Out of 32 (28.6%) Streptococcus isolates identified by phenotypic methods, 25 were genotypically identified as S. uberis, 5 as S. agalactiae, and 2 as S. dysgalactiae. Disk diffusion was used to determine the antimicrobial resistance of the isolates. PCR was employed to identify antimicrobial resistance and virulence genes in the isolates. The highest resistance was found for cloxacillin (100%), and the highest sensitivity was found for florfenicol (84%). The most common resistance gene combination was tetM+tetS (3/32) for S. uberis in 9.4%. A total of five virulence genes were detected. GapC+sua (56.2%) constituted the most common gene pattern. The highest virulence gene gapC was detected in 78.1% (25/32) of the isolates. The cylE gene was not detected (0%) in the isolates. Streptococcus species may play a role in mastitis in sheep, emphasising the need for meticulous hygienic milking practices.https://vetmed.agriculturejournals.cz/artkey/vet-202309-0002_evaluation-of-streptococcus-species-isolated-from-subclinical-sheep-mastitis-by-molecular-methods-and-determina.phpdairy sheepmastitispcrstreptococcus strains |
| spellingShingle | V Ozavci HT Yuksel Dolgun S Kirkan Y Seferoglu Z Semen U Parin Evaluation of Streptococcus species isolated from subclinical sheep mastitis by molecular methods and determination of virulence factors and antimicrobial resistance genes Veterinární Medicína dairy sheep mastitis pcr streptococcus strains |
| title | Evaluation of Streptococcus species isolated from subclinical sheep mastitis by molecular methods and determination of virulence factors and antimicrobial resistance genes |
| title_full | Evaluation of Streptococcus species isolated from subclinical sheep mastitis by molecular methods and determination of virulence factors and antimicrobial resistance genes |
| title_fullStr | Evaluation of Streptococcus species isolated from subclinical sheep mastitis by molecular methods and determination of virulence factors and antimicrobial resistance genes |
| title_full_unstemmed | Evaluation of Streptococcus species isolated from subclinical sheep mastitis by molecular methods and determination of virulence factors and antimicrobial resistance genes |
| title_short | Evaluation of Streptococcus species isolated from subclinical sheep mastitis by molecular methods and determination of virulence factors and antimicrobial resistance genes |
| title_sort | evaluation of streptococcus species isolated from subclinical sheep mastitis by molecular methods and determination of virulence factors and antimicrobial resistance genes |
| topic | dairy sheep mastitis pcr streptococcus strains |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-202309-0002_evaluation-of-streptococcus-species-isolated-from-subclinical-sheep-mastitis-by-molecular-methods-and-determina.php |
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