Adapting the Accelerated Solvent Extraction Method for Resin and Rubber Determination in Guayule Using the BÜCHI Speed Extractor
Guayule (<i>Parthenium argentatum</i> Gray) is a promising alternative source to <i>Hevea brasiliensis</i> for the production of natural rubber, which can reach levels of 8–9% under industrialized farming conditions. The most common method for determining rubber concentration...
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| Format: | Article |
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MDPI AG
2021-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/1/183 |
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| author | Juana Rozalén María de las Mercedes García-Martínez María Engracia Carrión Manuel Carmona Horacio López-Córcoles Katrina Cornish Amaya Zalacain |
| author_facet | Juana Rozalén María de las Mercedes García-Martínez María Engracia Carrión Manuel Carmona Horacio López-Córcoles Katrina Cornish Amaya Zalacain |
| author_sort | Juana Rozalén |
| collection | DOAJ |
| description | Guayule (<i>Parthenium argentatum</i> Gray) is a promising alternative source to <i>Hevea brasiliensis</i> for the production of natural rubber, which can reach levels of 8–9% under industrialized farming conditions. The most common method for determining rubber concentration is by accelerated solvent extraction (ASE), a technique developed by the Dionex Corporation and almost exclusively performed with the Dionex ASE-200 or 350 systems. Herein, it is sought to apply and adapt the most common methods used in the literature for the Dionex system to another extraction platform, the BÜCHI Speed Extractor E-914. Results showed that using a sand sandwich method to confine the sample in the center and exploiting a larger cell volume (80 mL) for extraction prevents the occurrence of overpressure and problems with clogging. Under optimized conditions, the coefficient of variation was <15% for both resin quantification for samples containing 5.0–15.8% of resin and for rubber quantification for samples with 1.7–10.3% rubber content. The extraction time for resin (2 cycles of 5 min each) was smaller than for rubber (2 cycles of 20 min each). It would be interesting to carry out interlaboratory comparisons to standardize the method at an international level. |
| first_indexed | 2024-03-10T13:32:39Z |
| format | Article |
| id | doaj.art-23fe2e04a0834102a5cdfe5081e98c3c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T13:32:39Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-23fe2e04a0834102a5cdfe5081e98c3c2023-11-21T07:43:58ZengMDPI AGMolecules1420-30492021-01-0126118310.3390/molecules26010183Adapting the Accelerated Solvent Extraction Method for Resin and Rubber Determination in Guayule Using the BÜCHI Speed ExtractorJuana Rozalén0María de las Mercedes García-Martínez1María Engracia Carrión2Manuel Carmona3Horacio López-Córcoles4Katrina Cornish5Amaya Zalacain6Catedra de Química Agrícola, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, Universidad de Castilla-La Mancha, Campus Universitario s/n, 02071 Albacete, SpainFood Quality Research Group, Institute for Regional Development (IDR), Universidad de Castilla-La Mancha, 02071 Albacete, SpainFood Technology Lab, School of Architecture, Engineering and Design, Universidad Europea de Madrid, C/Tajo s/n, 28670 Villaviciosa de Odón, SpainFood Quality Research Group, Institute for Regional Development (IDR), Universidad de Castilla-La Mancha, 02071 Albacete, SpainResearch Department, Instituto Técnico Agronómico Provincial de Albacete (ITAP), Parque Empresarial Campollano, 2<sup>a</sup> Avenida, 61, 02007 Albacete, SpainDepartments of Horticulture and Crop Science and of Food Agricultural and Biological Engineering, College of Food, Agricultural and Environmental Sciences, The Ohio State University, Wooster, OH 44691, USACatedra de Química Agrícola, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, Universidad de Castilla-La Mancha, Campus Universitario s/n, 02071 Albacete, SpainGuayule (<i>Parthenium argentatum</i> Gray) is a promising alternative source to <i>Hevea brasiliensis</i> for the production of natural rubber, which can reach levels of 8–9% under industrialized farming conditions. The most common method for determining rubber concentration is by accelerated solvent extraction (ASE), a technique developed by the Dionex Corporation and almost exclusively performed with the Dionex ASE-200 or 350 systems. Herein, it is sought to apply and adapt the most common methods used in the literature for the Dionex system to another extraction platform, the BÜCHI Speed Extractor E-914. Results showed that using a sand sandwich method to confine the sample in the center and exploiting a larger cell volume (80 mL) for extraction prevents the occurrence of overpressure and problems with clogging. Under optimized conditions, the coefficient of variation was <15% for both resin quantification for samples containing 5.0–15.8% of resin and for rubber quantification for samples with 1.7–10.3% rubber content. The extraction time for resin (2 cycles of 5 min each) was smaller than for rubber (2 cycles of 20 min each). It would be interesting to carry out interlaboratory comparisons to standardize the method at an international level.https://www.mdpi.com/1420-3049/26/1/183guayuleaccelerated solvent extraction (ASE)natural rubberresin |
| spellingShingle | Juana Rozalén María de las Mercedes García-Martínez María Engracia Carrión Manuel Carmona Horacio López-Córcoles Katrina Cornish Amaya Zalacain Adapting the Accelerated Solvent Extraction Method for Resin and Rubber Determination in Guayule Using the BÜCHI Speed Extractor Molecules guayule accelerated solvent extraction (ASE) natural rubber resin |
| title | Adapting the Accelerated Solvent Extraction Method for Resin and Rubber Determination in Guayule Using the BÜCHI Speed Extractor |
| title_full | Adapting the Accelerated Solvent Extraction Method for Resin and Rubber Determination in Guayule Using the BÜCHI Speed Extractor |
| title_fullStr | Adapting the Accelerated Solvent Extraction Method for Resin and Rubber Determination in Guayule Using the BÜCHI Speed Extractor |
| title_full_unstemmed | Adapting the Accelerated Solvent Extraction Method for Resin and Rubber Determination in Guayule Using the BÜCHI Speed Extractor |
| title_short | Adapting the Accelerated Solvent Extraction Method for Resin and Rubber Determination in Guayule Using the BÜCHI Speed Extractor |
| title_sort | adapting the accelerated solvent extraction method for resin and rubber determination in guayule using the buchi speed extractor |
| topic | guayule accelerated solvent extraction (ASE) natural rubber resin |
| url | https://www.mdpi.com/1420-3049/26/1/183 |
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