Influence of Glutamine and Branched-Chain Amino Acids Supplementation during Refeeding in Activity-Based Anorectic Mice
Background: Optimizing the refeeding of patients with anorexia nervosa remains important to limit somatic complications of malnutrition, as well as to avoid disease relapses by targeting persistent mood and intestinal disorders. We aimed to evaluate the effects of glutamine (Gln) and branched-chain...
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MDPI AG
2020-11-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/12/11/3510 |
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| author | Clément L’Huillier Marine Jarbeau Floriane Pingeon Wafa Bahlouli Emmeline Salameh Asma Amamou Alexis Goichon Pauline Tirelle Jean-Luc do Rego Pierre Déchelotte Moïse Coëffier |
| author_facet | Clément L’Huillier Marine Jarbeau Floriane Pingeon Wafa Bahlouli Emmeline Salameh Asma Amamou Alexis Goichon Pauline Tirelle Jean-Luc do Rego Pierre Déchelotte Moïse Coëffier |
| author_sort | Clément L’Huillier |
| collection | DOAJ |
| description | Background: Optimizing the refeeding of patients with anorexia nervosa remains important to limit somatic complications of malnutrition, as well as to avoid disease relapses by targeting persistent mood and intestinal disorders. We aimed to evaluate the effects of glutamine (Gln) and branched-chain amino acids (BCAA) supplementation during refeeding in activity-based anorectic (ABA) mice. Method: Male C57Bl/6 mice were randomized in control and ABA groups. Once ABA-induced malnutrition was established, mice were progressively refed or not. Refed mice had free access to drinking water supplemented or not with 1% Gln or 2.5% BCAA for 10 days. Results: A progressive refeeding was associated with a partial restoration of body weight and lean mass, while a fat mass rebound was observed. In addition, refeeding restored glucose and leptin. Gln did not affect these parameters, while BCAA tended to increase body weight, fat mass, and glycaemia. In the colon, refeeding improved total protein synthesis and restored the LC3II/LC3I ratio, a marker of autophagy. Gln supplementation enhanced colonic protein synthesis, which was associated with an increased p-p70S6kinase/p70S6kinase ratio, whereas these effects were blunted by BCCA supplementation. Conclusions: In ABA mice, Gln and BCAA supplementations during a progressive refeeding fail to restore body weight and lean mass. However, Gln supplementation improves total colonic protein synthesis conversely to BCAA. Further studies are needed to decipher the underlying mechanisms involved in these opposite results. |
| first_indexed | 2024-03-10T14:50:28Z |
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| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T14:50:28Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-24078ae03f1144c9857ded13c994ee212023-11-20T20:58:28ZengMDPI AGNutrients2072-66432020-11-011211351010.3390/nu12113510Influence of Glutamine and Branched-Chain Amino Acids Supplementation during Refeeding in Activity-Based Anorectic MiceClément L’Huillier0Marine Jarbeau1Floriane Pingeon2Wafa Bahlouli3Emmeline Salameh4Asma Amamou5Alexis Goichon6Pauline Tirelle7Jean-Luc do Rego8Pierre Déchelotte9Moïse Coëffier10Normandie University, UNIROUEN, INSERM UMR 1073 Nutrition, Inflammation and Gut-Brain Axis, 76000 Rouen, FranceNormandie University, UNIROUEN, INSERM UMR 1073 Nutrition, Inflammation and Gut-Brain Axis, 76000 Rouen, FranceNormandie University, UNIROUEN, INSERM UMR 1073 Nutrition, Inflammation and Gut-Brain Axis, 76000 Rouen, FranceNormandie University, UNIROUEN, INSERM UMR 1073 Nutrition, Inflammation and Gut-Brain Axis, 76000 Rouen, FranceNormandie University, UNIROUEN, INSERM UMR 1073 Nutrition, Inflammation and Gut-Brain Axis, 76000 Rouen, FranceNormandie University, UNIROUEN, INSERM UMR 1073 Nutrition, Inflammation and Gut-Brain Axis, 76000 Rouen, FranceNormandie University, UNIROUEN, INSERM UMR 1073 Nutrition, Inflammation and Gut-Brain Axis, 76000 Rouen, FranceNormandie University, UNIROUEN, INSERM UMR 1073 Nutrition, Inflammation and Gut-Brain Axis, 76000 Rouen, FranceInstitute of Research and Innovation in Biomedicine (IRIB), UNIROUEN, 76000 Rouen, FranceNormandie University, UNIROUEN, INSERM UMR 1073 Nutrition, Inflammation and Gut-Brain Axis, 76000 Rouen, FranceNormandie University, UNIROUEN, INSERM UMR 1073 Nutrition, Inflammation and Gut-Brain Axis, 76000 Rouen, FranceBackground: Optimizing the refeeding of patients with anorexia nervosa remains important to limit somatic complications of malnutrition, as well as to avoid disease relapses by targeting persistent mood and intestinal disorders. We aimed to evaluate the effects of glutamine (Gln) and branched-chain amino acids (BCAA) supplementation during refeeding in activity-based anorectic (ABA) mice. Method: Male C57Bl/6 mice were randomized in control and ABA groups. Once ABA-induced malnutrition was established, mice were progressively refed or not. Refed mice had free access to drinking water supplemented or not with 1% Gln or 2.5% BCAA for 10 days. Results: A progressive refeeding was associated with a partial restoration of body weight and lean mass, while a fat mass rebound was observed. In addition, refeeding restored glucose and leptin. Gln did not affect these parameters, while BCAA tended to increase body weight, fat mass, and glycaemia. In the colon, refeeding improved total protein synthesis and restored the LC3II/LC3I ratio, a marker of autophagy. Gln supplementation enhanced colonic protein synthesis, which was associated with an increased p-p70S6kinase/p70S6kinase ratio, whereas these effects were blunted by BCCA supplementation. Conclusions: In ABA mice, Gln and BCAA supplementations during a progressive refeeding fail to restore body weight and lean mass. However, Gln supplementation improves total colonic protein synthesis conversely to BCAA. Further studies are needed to decipher the underlying mechanisms involved in these opposite results.https://www.mdpi.com/2072-6643/12/11/3510activity-based anorexiaglutaminebranched-chain amino acidsrefeedingprotein synthesiscolon |
| spellingShingle | Clément L’Huillier Marine Jarbeau Floriane Pingeon Wafa Bahlouli Emmeline Salameh Asma Amamou Alexis Goichon Pauline Tirelle Jean-Luc do Rego Pierre Déchelotte Moïse Coëffier Influence of Glutamine and Branched-Chain Amino Acids Supplementation during Refeeding in Activity-Based Anorectic Mice Nutrients activity-based anorexia glutamine branched-chain amino acids refeeding protein synthesis colon |
| title | Influence of Glutamine and Branched-Chain Amino Acids Supplementation during Refeeding in Activity-Based Anorectic Mice |
| title_full | Influence of Glutamine and Branched-Chain Amino Acids Supplementation during Refeeding in Activity-Based Anorectic Mice |
| title_fullStr | Influence of Glutamine and Branched-Chain Amino Acids Supplementation during Refeeding in Activity-Based Anorectic Mice |
| title_full_unstemmed | Influence of Glutamine and Branched-Chain Amino Acids Supplementation during Refeeding in Activity-Based Anorectic Mice |
| title_short | Influence of Glutamine and Branched-Chain Amino Acids Supplementation during Refeeding in Activity-Based Anorectic Mice |
| title_sort | influence of glutamine and branched chain amino acids supplementation during refeeding in activity based anorectic mice |
| topic | activity-based anorexia glutamine branched-chain amino acids refeeding protein synthesis colon |
| url | https://www.mdpi.com/2072-6643/12/11/3510 |
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