Quantum-Dot-Based Iron Oxide Nanoparticles Activate the NLRP3 Inflammasome in Murine Bone Marrow-Derived Dendritic Cells
Inflammasomes are cytosolic complexes composed of a Nod-like receptor, NLR, the adaptor protein, ASC, and a proteolytic enzyme, caspase-1. Inflammasome activation leads to caspase-1 activation and promotes functional maturation of IL-1β and IL-18, two prototypical inflammatory cytokines. Besides, in...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/18/3145 |
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| author | Fernando Menegatti de Melo Karine Kawasaki Tarciso Almeida Sellani Bruno Souza Bonifácio Renato Arruda Mortara Henrique Eisi Toma Filipe Menegatti de Melo Elaine Guadelupe Rodrigues |
| author_facet | Fernando Menegatti de Melo Karine Kawasaki Tarciso Almeida Sellani Bruno Souza Bonifácio Renato Arruda Mortara Henrique Eisi Toma Filipe Menegatti de Melo Elaine Guadelupe Rodrigues |
| author_sort | Fernando Menegatti de Melo |
| collection | DOAJ |
| description | Inflammasomes are cytosolic complexes composed of a Nod-like receptor, NLR, the adaptor protein, ASC, and a proteolytic enzyme, caspase-1. Inflammasome activation leads to caspase-1 activation and promotes functional maturation of IL-1β and IL-18, two prototypical inflammatory cytokines. Besides, inflammasome activation leads to pyroptosis, an inflammatory type of cell death. Inflammasomes are vital for the host to cope with foreign pathogens or tissue damage. Herein, we show that quantum-dot-based iron oxide nanoparticles, MNP@QD, trigger NLRP3 inflammasome activation and subsequent release of proinflammatory interleukin IL-1β by murine bone marrow-derived dendritic cells (BMDCs). This activation is more pronounced if these cells endocytose the nanoparticles before receiving inflammatory stimulation. MNP@QD was characterized by using imaging techniques like transmission electron microscopy, fluorescence microscopy, and atomic force microscopy, as well as physical and spectroscopical techniques such as fluorescence spectroscopy and powder diffraction. These findings may open the possibility of using the composite MNP@QD as both an imaging and a therapeutic tool. |
| first_indexed | 2024-03-09T22:57:50Z |
| format | Article |
| id | doaj.art-2408d5d1227c4b05943717994f1363ce |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T22:57:50Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-2408d5d1227c4b05943717994f1363ce2023-11-23T18:06:15ZengMDPI AGNanomaterials2079-49912022-09-011218314510.3390/nano12183145Quantum-Dot-Based Iron Oxide Nanoparticles Activate the NLRP3 Inflammasome in Murine Bone Marrow-Derived Dendritic CellsFernando Menegatti de Melo0Karine Kawasaki1Tarciso Almeida Sellani2Bruno Souza Bonifácio3Renato Arruda Mortara4Henrique Eisi Toma5Filipe Menegatti de Melo6Elaine Guadelupe Rodrigues7Department of Chemistry, Institute of Chemistry, University of São Paulo (USP), Av. Lineu Prestes 748, Butantã, São Paulo 05508-000, SP, BrazilDepartment of Microbiology, Immunology and Parasitology, Federal University of São Paulo (UNIFESP), Rua Botucatu, 862, Vila Clementino, São Paulo 04023-062, SP, BrazilDepartment of Microbiology, Immunology and Parasitology, Federal University of São Paulo (UNIFESP), Rua Botucatu, 862, Vila Clementino, São Paulo 04023-062, SP, BrazilDepartment of Microbiology, Immunology and Parasitology, Federal University of São Paulo (UNIFESP), Rua Botucatu, 862, Vila Clementino, São Paulo 04023-062, SP, BrazilDepartment of Microbiology, Immunology and Parasitology, Federal University of São Paulo (UNIFESP), Rua Botucatu, 862, Vila Clementino, São Paulo 04023-062, SP, BrazilDepartment of Chemistry, Institute of Chemistry, University of São Paulo (USP), Av. Lineu Prestes 748, Butantã, São Paulo 05508-000, SP, BrazilDepartment of Microbiology, Immunology and Parasitology, Federal University of São Paulo (UNIFESP), Rua Botucatu, 862, Vila Clementino, São Paulo 04023-062, SP, BrazilDepartment of Microbiology, Immunology and Parasitology, Federal University of São Paulo (UNIFESP), Rua Botucatu, 862, Vila Clementino, São Paulo 04023-062, SP, BrazilInflammasomes are cytosolic complexes composed of a Nod-like receptor, NLR, the adaptor protein, ASC, and a proteolytic enzyme, caspase-1. Inflammasome activation leads to caspase-1 activation and promotes functional maturation of IL-1β and IL-18, two prototypical inflammatory cytokines. Besides, inflammasome activation leads to pyroptosis, an inflammatory type of cell death. Inflammasomes are vital for the host to cope with foreign pathogens or tissue damage. Herein, we show that quantum-dot-based iron oxide nanoparticles, MNP@QD, trigger NLRP3 inflammasome activation and subsequent release of proinflammatory interleukin IL-1β by murine bone marrow-derived dendritic cells (BMDCs). This activation is more pronounced if these cells endocytose the nanoparticles before receiving inflammatory stimulation. MNP@QD was characterized by using imaging techniques like transmission electron microscopy, fluorescence microscopy, and atomic force microscopy, as well as physical and spectroscopical techniques such as fluorescence spectroscopy and powder diffraction. These findings may open the possibility of using the composite MNP@QD as both an imaging and a therapeutic tool.https://www.mdpi.com/2079-4991/12/18/3145quantum-dotiron oxide nanoparticlesNLRP inflammasomedendritic cells |
| spellingShingle | Fernando Menegatti de Melo Karine Kawasaki Tarciso Almeida Sellani Bruno Souza Bonifácio Renato Arruda Mortara Henrique Eisi Toma Filipe Menegatti de Melo Elaine Guadelupe Rodrigues Quantum-Dot-Based Iron Oxide Nanoparticles Activate the NLRP3 Inflammasome in Murine Bone Marrow-Derived Dendritic Cells Nanomaterials quantum-dot iron oxide nanoparticles NLRP inflammasome dendritic cells |
| title | Quantum-Dot-Based Iron Oxide Nanoparticles Activate the NLRP3 Inflammasome in Murine Bone Marrow-Derived Dendritic Cells |
| title_full | Quantum-Dot-Based Iron Oxide Nanoparticles Activate the NLRP3 Inflammasome in Murine Bone Marrow-Derived Dendritic Cells |
| title_fullStr | Quantum-Dot-Based Iron Oxide Nanoparticles Activate the NLRP3 Inflammasome in Murine Bone Marrow-Derived Dendritic Cells |
| title_full_unstemmed | Quantum-Dot-Based Iron Oxide Nanoparticles Activate the NLRP3 Inflammasome in Murine Bone Marrow-Derived Dendritic Cells |
| title_short | Quantum-Dot-Based Iron Oxide Nanoparticles Activate the NLRP3 Inflammasome in Murine Bone Marrow-Derived Dendritic Cells |
| title_sort | quantum dot based iron oxide nanoparticles activate the nlrp3 inflammasome in murine bone marrow derived dendritic cells |
| topic | quantum-dot iron oxide nanoparticles NLRP inflammasome dendritic cells |
| url | https://www.mdpi.com/2079-4991/12/18/3145 |
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