Polyvinylpyrrolidone (PVP) and Na-Alginate Addition Enhances the Survival and Agronomic Performances of a Liquid Inoculant of <i>Bradyrhizobium japonicum</i> for Soybean (<i>Glycine max</i> (L.) <i>Merr.</i>)
Nontoxic polymers PVP and Na-alginate may provide a favorable environment for the survival of bacteria. Therefore, PVP and Na-alginate were added to a growth medium to develop a liquid inoculant of <i>Bradyrhizobium japonicum</i> strain. The strain was identified by 16S rDNA sequencing....
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| Format: | Article |
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MDPI AG
2021-05-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/11/5/1009 |
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| author | Pulak Maitra Jubair Al-Rashid Dipa Mandal Md. Shofiul Azam Noorain Munim Rasul |
| author_facet | Pulak Maitra Jubair Al-Rashid Dipa Mandal Md. Shofiul Azam Noorain Munim Rasul |
| author_sort | Pulak Maitra |
| collection | DOAJ |
| description | Nontoxic polymers PVP and Na-alginate may provide a favorable environment for the survival of bacteria. Therefore, PVP and Na-alginate were added to a growth medium to develop a liquid inoculant of <i>Bradyrhizobium japonicum</i> strain. The strain was identified by 16S rDNA sequencing. The addition of PVP (1.8%) and Na-alginate (0.2%) in the medium promoted a better survival (1.93 × 10<sup>9</sup> cells mL<sup>−1</sup>) of <i>B. japonicum</i> strain compared to the control (3.50 × 10<sup>2</sup> cells mL<sup>−1</sup>) after 6 months of storage. The combination of PVP and Na-alginate ensured 1.53 × 10<sup>7</sup> cells mL<sup>−1</sup> up to 12 months of storage under ambient temperature (28 ± 2 °C), whereas PVP (1.8%) or Na-alginate (0.2%) alone produced similar cell counts only up to 8 months and 6 months, respectively. Consecutive field experiments proved the efficacy of the liquid inoculant on nodulation and yield of soybean. The combination of PVP and Na-alginate-based inoculation of <i>B. japonicum</i> strain significantly increased the nodule number per plant, number of pods per plant, number of seeds per pod, seed yield, and yield per hectare (<i>p</i> ≤ 0.05). Thus, the combination of PVP- and Na-alginate-based inoculation of <i>B. japonicum</i> has great potential to popularize the organic cultivation of soybean. |
| first_indexed | 2024-03-10T11:13:56Z |
| format | Article |
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| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T11:13:56Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
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| series | Agronomy |
| spelling | doaj.art-240a6ad3144d452b9525ea82ce65402f2023-11-21T20:31:50ZengMDPI AGAgronomy2073-43952021-05-01115100910.3390/agronomy11051009Polyvinylpyrrolidone (PVP) and Na-Alginate Addition Enhances the Survival and Agronomic Performances of a Liquid Inoculant of <i>Bradyrhizobium japonicum</i> for Soybean (<i>Glycine max</i> (L.) <i>Merr.</i>)Pulak Maitra0Jubair Al-Rashid1Dipa Mandal2Md. Shofiul Azam3Noorain Munim Rasul4Solid-State Fermentation Resource Utilization Key Laboratory of Sichuan Province, Faculty of Agriculture, Forestry and Food Engineering, Yibin University, Yibin 644000, ChinaApex Biotechnology Laboratory, Apex Holdings Ltd., East Chandora, Shafipur, Kaliakoir, Gazipur 1751, BangladeshSchool of International Education, University of Chinese Academy of Sciences, Beijing 100049, ChinaSchool of International Education, University of Chinese Academy of Sciences, Beijing 100049, ChinaApex Biotechnology Laboratory, Apex Holdings Ltd., East Chandora, Shafipur, Kaliakoir, Gazipur 1751, BangladeshNontoxic polymers PVP and Na-alginate may provide a favorable environment for the survival of bacteria. Therefore, PVP and Na-alginate were added to a growth medium to develop a liquid inoculant of <i>Bradyrhizobium japonicum</i> strain. The strain was identified by 16S rDNA sequencing. The addition of PVP (1.8%) and Na-alginate (0.2%) in the medium promoted a better survival (1.93 × 10<sup>9</sup> cells mL<sup>−1</sup>) of <i>B. japonicum</i> strain compared to the control (3.50 × 10<sup>2</sup> cells mL<sup>−1</sup>) after 6 months of storage. The combination of PVP and Na-alginate ensured 1.53 × 10<sup>7</sup> cells mL<sup>−1</sup> up to 12 months of storage under ambient temperature (28 ± 2 °C), whereas PVP (1.8%) or Na-alginate (0.2%) alone produced similar cell counts only up to 8 months and 6 months, respectively. Consecutive field experiments proved the efficacy of the liquid inoculant on nodulation and yield of soybean. The combination of PVP and Na-alginate-based inoculation of <i>B. japonicum</i> strain significantly increased the nodule number per plant, number of pods per plant, number of seeds per pod, seed yield, and yield per hectare (<i>p</i> ≤ 0.05). Thus, the combination of PVP- and Na-alginate-based inoculation of <i>B. japonicum</i> has great potential to popularize the organic cultivation of soybean.https://www.mdpi.com/2073-4395/11/5/1009<i>Bradyrhizobium japonicum</i>biofertilizerssoybeannodulationcrop yield |
| spellingShingle | Pulak Maitra Jubair Al-Rashid Dipa Mandal Md. Shofiul Azam Noorain Munim Rasul Polyvinylpyrrolidone (PVP) and Na-Alginate Addition Enhances the Survival and Agronomic Performances of a Liquid Inoculant of <i>Bradyrhizobium japonicum</i> for Soybean (<i>Glycine max</i> (L.) <i>Merr.</i>) Agronomy <i>Bradyrhizobium japonicum</i> biofertilizers soybean nodulation crop yield |
| title | Polyvinylpyrrolidone (PVP) and Na-Alginate Addition Enhances the Survival and Agronomic Performances of a Liquid Inoculant of <i>Bradyrhizobium japonicum</i> for Soybean (<i>Glycine max</i> (L.) <i>Merr.</i>) |
| title_full | Polyvinylpyrrolidone (PVP) and Na-Alginate Addition Enhances the Survival and Agronomic Performances of a Liquid Inoculant of <i>Bradyrhizobium japonicum</i> for Soybean (<i>Glycine max</i> (L.) <i>Merr.</i>) |
| title_fullStr | Polyvinylpyrrolidone (PVP) and Na-Alginate Addition Enhances the Survival and Agronomic Performances of a Liquid Inoculant of <i>Bradyrhizobium japonicum</i> for Soybean (<i>Glycine max</i> (L.) <i>Merr.</i>) |
| title_full_unstemmed | Polyvinylpyrrolidone (PVP) and Na-Alginate Addition Enhances the Survival and Agronomic Performances of a Liquid Inoculant of <i>Bradyrhizobium japonicum</i> for Soybean (<i>Glycine max</i> (L.) <i>Merr.</i>) |
| title_short | Polyvinylpyrrolidone (PVP) and Na-Alginate Addition Enhances the Survival and Agronomic Performances of a Liquid Inoculant of <i>Bradyrhizobium japonicum</i> for Soybean (<i>Glycine max</i> (L.) <i>Merr.</i>) |
| title_sort | polyvinylpyrrolidone pvp and na alginate addition enhances the survival and agronomic performances of a liquid inoculant of i bradyrhizobium japonicum i for soybean i glycine max i l i merr i |
| topic | <i>Bradyrhizobium japonicum</i> biofertilizers soybean nodulation crop yield |
| url | https://www.mdpi.com/2073-4395/11/5/1009 |
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