Salivary Gland Tissue Engineering Approaches: State of the Art and Future Directions
Salivary gland regeneration is important for developing treatments for radiation-induced xerostomia, Sjögren’s syndrome, and other conditions that cause dry mouth. Culture conditions adopted from tissue engineering strategies have been used to recapitulate gland structure and function to study and r...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/10/7/1723 |
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| author | Lindsay R. Piraino Danielle S. W. Benoit Lisa A. DeLouise |
| author_facet | Lindsay R. Piraino Danielle S. W. Benoit Lisa A. DeLouise |
| author_sort | Lindsay R. Piraino |
| collection | DOAJ |
| description | Salivary gland regeneration is important for developing treatments for radiation-induced xerostomia, Sjögren’s syndrome, and other conditions that cause dry mouth. Culture conditions adopted from tissue engineering strategies have been used to recapitulate gland structure and function to study and regenerate the salivary glands. The purpose of this review is to highlight current trends in the field, with an emphasis on soluble factors that have been shown to improve secretory function in vitro. A PubMed search was conducted to identify articles published in the last 10 years and articles were evaluated to identify the most promising approaches and areas for further research. Results showed increasing use of extracellular matrix mimetics, such as Matrigel<sup>®</sup>, collagen, and a variety of functionalized polymers. Soluble factors that provide supportive cues, including fibroblast growth factors (FGFs) and neurotrophic factors, as well as chemical inhibitors of Rho-associated kinase (ROCK), epidermal growth factor receptor (EGFR), and transforming growth factor β receptor (TGFβR) have shown increases in important markers including aquaporin 5 (Aqp5); muscle, intestine, and stomach expression 1 (Mist1); and keratin (K5). However, recapitulation of tissue function at in vivo levels is still elusive. A focus on identification of soluble factors, cells, and/or matrix cues tested in combination may further increase the maintenance of salivary gland secretory function in vitro. These approaches may also be amenable for translation in vivo to support successful regeneration of dysfunctional glands. |
| first_indexed | 2024-03-10T09:42:53Z |
| format | Article |
| id | doaj.art-240f2d6b4edf4f40840575c00e6f8f2f |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T09:42:53Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-240f2d6b4edf4f40840575c00e6f8f2f2023-11-22T03:29:00ZengMDPI AGCells2073-44092021-07-01107172310.3390/cells10071723Salivary Gland Tissue Engineering Approaches: State of the Art and Future DirectionsLindsay R. Piraino0Danielle S. W. Benoit1Lisa A. DeLouise2Department of Biomedical Engineering, University of Rochester, Rochester, NY 14627, USADepartment of Biomedical Engineering, University of Rochester, Rochester, NY 14627, USADepartment of Biomedical Engineering, University of Rochester, Rochester, NY 14627, USASalivary gland regeneration is important for developing treatments for radiation-induced xerostomia, Sjögren’s syndrome, and other conditions that cause dry mouth. Culture conditions adopted from tissue engineering strategies have been used to recapitulate gland structure and function to study and regenerate the salivary glands. The purpose of this review is to highlight current trends in the field, with an emphasis on soluble factors that have been shown to improve secretory function in vitro. A PubMed search was conducted to identify articles published in the last 10 years and articles were evaluated to identify the most promising approaches and areas for further research. Results showed increasing use of extracellular matrix mimetics, such as Matrigel<sup>®</sup>, collagen, and a variety of functionalized polymers. Soluble factors that provide supportive cues, including fibroblast growth factors (FGFs) and neurotrophic factors, as well as chemical inhibitors of Rho-associated kinase (ROCK), epidermal growth factor receptor (EGFR), and transforming growth factor β receptor (TGFβR) have shown increases in important markers including aquaporin 5 (Aqp5); muscle, intestine, and stomach expression 1 (Mist1); and keratin (K5). However, recapitulation of tissue function at in vivo levels is still elusive. A focus on identification of soluble factors, cells, and/or matrix cues tested in combination may further increase the maintenance of salivary gland secretory function in vitro. These approaches may also be amenable for translation in vivo to support successful regeneration of dysfunctional glands.https://www.mdpi.com/2073-4409/10/7/1723salivary glandtissue engineeringcell culturesoluble cuesmedia optimization |
| spellingShingle | Lindsay R. Piraino Danielle S. W. Benoit Lisa A. DeLouise Salivary Gland Tissue Engineering Approaches: State of the Art and Future Directions Cells salivary gland tissue engineering cell culture soluble cues media optimization |
| title | Salivary Gland Tissue Engineering Approaches: State of the Art and Future Directions |
| title_full | Salivary Gland Tissue Engineering Approaches: State of the Art and Future Directions |
| title_fullStr | Salivary Gland Tissue Engineering Approaches: State of the Art and Future Directions |
| title_full_unstemmed | Salivary Gland Tissue Engineering Approaches: State of the Art and Future Directions |
| title_short | Salivary Gland Tissue Engineering Approaches: State of the Art and Future Directions |
| title_sort | salivary gland tissue engineering approaches state of the art and future directions |
| topic | salivary gland tissue engineering cell culture soluble cues media optimization |
| url | https://www.mdpi.com/2073-4409/10/7/1723 |
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