Therapeutic Potential of Engineered Virus-like Particles of Parvovirus B19
Virus-like particles (VLPs) comprise one or many structural components of virions, except their genetic material. Thus, VLPs keep their structural properties of cellular recognition while being non-infectious. VLPs of Parvovirus B19 (B19V) can be produced by the heterologous expression of their stru...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/12/8/1007 |
| _version_ | 1797583539253805056 |
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| author | Ignacio Sánchez-Moguel Carmina Montiel Ismael Bustos-Jaimes |
| author_facet | Ignacio Sánchez-Moguel Carmina Montiel Ismael Bustos-Jaimes |
| author_sort | Ignacio Sánchez-Moguel |
| collection | DOAJ |
| description | Virus-like particles (VLPs) comprise one or many structural components of virions, except their genetic material. Thus, VLPs keep their structural properties of cellular recognition while being non-infectious. VLPs of Parvovirus B19 (B19V) can be produced by the heterologous expression of their structural proteins VP1 and VP2 in bacteria. These proteins are purified under denaturing conditions, refolded, and assembled into VLPs. Moreover, chimeric forms of VP2 have been constructed to harbor peptides or functional proteins on the surface of the particles without dropping their competence to form VLPs, serving as presenting nanoparticles. The in-vitro assembly approach offers exciting possibilities for the composition of VLPs, as more than one chimeric form of VP2 can be included in the assembly stage, producing multifunctional VLPs. Here, the heterologous expression and in-vitro assembly of B19V structural proteins and their chimeras are reviewed. Considerations for the engineering of the structural proteins of B19V are also discussed. Finally, the construction of multifunctional VLPs and their future potential as innovative medical tools are examined. |
| first_indexed | 2024-03-10T23:40:18Z |
| format | Article |
| id | doaj.art-241300a218b2405598d536a3002a084f |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T23:40:18Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-241300a218b2405598d536a3002a084f2023-11-19T02:32:08ZengMDPI AGPathogens2076-08172023-08-01128100710.3390/pathogens12081007Therapeutic Potential of Engineered Virus-like Particles of Parvovirus B19Ignacio Sánchez-Moguel0Carmina Montiel1Ismael Bustos-Jaimes2Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Mexico City 04510, MexicoDepartamento de Alimentos y Biotecnología, Facultad de Química, Universidad Nacional Autónoma de México (UNAM), Mexico City 04510, MexicoDepartamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Mexico City 04510, MexicoVirus-like particles (VLPs) comprise one or many structural components of virions, except their genetic material. Thus, VLPs keep their structural properties of cellular recognition while being non-infectious. VLPs of Parvovirus B19 (B19V) can be produced by the heterologous expression of their structural proteins VP1 and VP2 in bacteria. These proteins are purified under denaturing conditions, refolded, and assembled into VLPs. Moreover, chimeric forms of VP2 have been constructed to harbor peptides or functional proteins on the surface of the particles without dropping their competence to form VLPs, serving as presenting nanoparticles. The in-vitro assembly approach offers exciting possibilities for the composition of VLPs, as more than one chimeric form of VP2 can be included in the assembly stage, producing multifunctional VLPs. Here, the heterologous expression and in-vitro assembly of B19V structural proteins and their chimeras are reviewed. Considerations for the engineering of the structural proteins of B19V are also discussed. Finally, the construction of multifunctional VLPs and their future potential as innovative medical tools are examined.https://www.mdpi.com/2076-0817/12/8/1007parvovirus-like particlesprotein engineeringenzyme nanocarriersnanobiotecnologynanomedicine |
| spellingShingle | Ignacio Sánchez-Moguel Carmina Montiel Ismael Bustos-Jaimes Therapeutic Potential of Engineered Virus-like Particles of Parvovirus B19 Pathogens parvovirus-like particles protein engineering enzyme nanocarriers nanobiotecnology nanomedicine |
| title | Therapeutic Potential of Engineered Virus-like Particles of Parvovirus B19 |
| title_full | Therapeutic Potential of Engineered Virus-like Particles of Parvovirus B19 |
| title_fullStr | Therapeutic Potential of Engineered Virus-like Particles of Parvovirus B19 |
| title_full_unstemmed | Therapeutic Potential of Engineered Virus-like Particles of Parvovirus B19 |
| title_short | Therapeutic Potential of Engineered Virus-like Particles of Parvovirus B19 |
| title_sort | therapeutic potential of engineered virus like particles of parvovirus b19 |
| topic | parvovirus-like particles protein engineering enzyme nanocarriers nanobiotecnology nanomedicine |
| url | https://www.mdpi.com/2076-0817/12/8/1007 |
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