Worenine Prevents Solar Ultraviolet–Induced Sunburn by Inhibiting JNK2
Excessive solar ultraviolet (SUV) radiation often causes dermatitis, photoaging, and even skin cancer. In the pathological processes of SUV-induced sunburn, JNK is activated by phosphorylation, and it in turn phosphorylates its downstream transcription factors, such as ATF2 and c-jun. The transcript...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-07-01
|
| Series: | Frontiers in Pharmacology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.881042/full |
| _version_ | 1818179869484253184 |
|---|---|
| author | Juanjuan Xiao Juanjuan Xiao Hui Lu Hui Lu Tengfei Ma Xiaofang Ni Teding Chang Man Liu Nijie Li Peijiang Lu Changshu Ke Qin Tian Ling Zou Fei Wang Wei Wang Lu Zhang Ping Yuan Lin Liu Jianmin Zhang Fei Shi Qiuhong Duan Feng Zhu Feng Zhu Feng Zhu Feng Zhu |
| author_facet | Juanjuan Xiao Juanjuan Xiao Hui Lu Hui Lu Tengfei Ma Xiaofang Ni Teding Chang Man Liu Nijie Li Peijiang Lu Changshu Ke Qin Tian Ling Zou Fei Wang Wei Wang Lu Zhang Ping Yuan Lin Liu Jianmin Zhang Fei Shi Qiuhong Duan Feng Zhu Feng Zhu Feng Zhu Feng Zhu |
| author_sort | Juanjuan Xiao |
| collection | DOAJ |
| description | Excessive solar ultraviolet (SUV) radiation often causes dermatitis, photoaging, and even skin cancer. In the pathological processes of SUV-induced sunburn, JNK is activated by phosphorylation, and it in turn phosphorylates its downstream transcription factors, such as ATF2 and c-jun. The transcription factors further regulate the expression of pro-inflammatory genes, such as IL-6 and TNF-α, which ultimately leads to dermatitis. Therefore, inhibiting JNK may be a strategy to prevent dermatitis. In this study, we screened for worenine as a potential drug candidate for inhibiting sunburn. We determined that worenine inhibited the JNK-ATF2/c-jun signaling pathway and the secretion of IL-6 and TNF-α in cell culture and in vivo, confirming the role of worenine in inhibiting sunburn. Furthermore, we determined that worenine bound and inhibited JNK2 activity in vitro through the MST, kinase, and in vitro kinase assays. Therefore, worenine might be a promising drug candidate for the prevention and treatment of SUV-induced sunburn. |
| first_indexed | 2024-12-11T21:10:44Z |
| format | Article |
| id | doaj.art-24134b29180d4992bffef8021c37ed80 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-11T21:10:44Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-24134b29180d4992bffef8021c37ed802022-12-22T00:50:43ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-07-011310.3389/fphar.2022.881042881042Worenine Prevents Solar Ultraviolet–Induced Sunburn by Inhibiting JNK2Juanjuan Xiao0Juanjuan Xiao1Hui Lu2Hui Lu3Tengfei Ma4Xiaofang Ni5Teding Chang6Man Liu7Nijie Li8Peijiang Lu9Changshu Ke10Qin Tian11Ling Zou12Fei Wang13Wei Wang14Lu Zhang15Ping Yuan16Lin Liu17Jianmin Zhang18Fei Shi19Qiuhong Duan20Feng Zhu21Feng Zhu22Feng Zhu23Feng Zhu24Cancer Research Institute, The Affiliated Hospital of Guilin Medical University, Guilin, ChinaGuangxi Health Commission Key Laboratory of Novel Onco-Kinases in Target Therapy, The Affiliated Hospital of Guilin Medical University, Guilin, ChinaWuhan Children’s Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaWuhan Children’s Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaSecond Clinical College, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaSecond Clinical College, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaSecond Clinical College, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaSecond Clinical College, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Dermatology, The General Hospital of Air Force, Beijing, ChinaDepartment of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaCancer Research Institute, The Affiliated Hospital of Guilin Medical University, Guilin, ChinaGuangxi Health Commission Key Laboratory of Novel Onco-Kinases in Target Therapy, The Affiliated Hospital of Guilin Medical University, Guilin, ChinaDepartment of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaGuangxi Key Laboratory of Molecular Medicine in Liver Injury and Repair, The Affiliated Hospital of Guilin Medical University, Guilin, ChinaExcessive solar ultraviolet (SUV) radiation often causes dermatitis, photoaging, and even skin cancer. In the pathological processes of SUV-induced sunburn, JNK is activated by phosphorylation, and it in turn phosphorylates its downstream transcription factors, such as ATF2 and c-jun. The transcription factors further regulate the expression of pro-inflammatory genes, such as IL-6 and TNF-α, which ultimately leads to dermatitis. Therefore, inhibiting JNK may be a strategy to prevent dermatitis. In this study, we screened for worenine as a potential drug candidate for inhibiting sunburn. We determined that worenine inhibited the JNK-ATF2/c-jun signaling pathway and the secretion of IL-6 and TNF-α in cell culture and in vivo, confirming the role of worenine in inhibiting sunburn. Furthermore, we determined that worenine bound and inhibited JNK2 activity in vitro through the MST, kinase, and in vitro kinase assays. Therefore, worenine might be a promising drug candidate for the prevention and treatment of SUV-induced sunburn.https://www.frontiersin.org/articles/10.3389/fphar.2022.881042/fullTCMtraditional Chinese medicineworenineJNK2SUVsunburn |
| spellingShingle | Juanjuan Xiao Juanjuan Xiao Hui Lu Hui Lu Tengfei Ma Xiaofang Ni Teding Chang Man Liu Nijie Li Peijiang Lu Changshu Ke Qin Tian Ling Zou Fei Wang Wei Wang Lu Zhang Ping Yuan Lin Liu Jianmin Zhang Fei Shi Qiuhong Duan Feng Zhu Feng Zhu Feng Zhu Feng Zhu Worenine Prevents Solar Ultraviolet–Induced Sunburn by Inhibiting JNK2 Frontiers in Pharmacology TCM traditional Chinese medicine worenine JNK2 SUV sunburn |
| title | Worenine Prevents Solar Ultraviolet–Induced Sunburn by Inhibiting JNK2 |
| title_full | Worenine Prevents Solar Ultraviolet–Induced Sunburn by Inhibiting JNK2 |
| title_fullStr | Worenine Prevents Solar Ultraviolet–Induced Sunburn by Inhibiting JNK2 |
| title_full_unstemmed | Worenine Prevents Solar Ultraviolet–Induced Sunburn by Inhibiting JNK2 |
| title_short | Worenine Prevents Solar Ultraviolet–Induced Sunburn by Inhibiting JNK2 |
| title_sort | worenine prevents solar ultraviolet induced sunburn by inhibiting jnk2 |
| topic | TCM traditional Chinese medicine worenine JNK2 SUV sunburn |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.881042/full |
| work_keys_str_mv | AT juanjuanxiao woreninepreventssolarultravioletinducedsunburnbyinhibitingjnk2 AT juanjuanxiao woreninepreventssolarultravioletinducedsunburnbyinhibitingjnk2 AT huilu woreninepreventssolarultravioletinducedsunburnbyinhibitingjnk2 AT huilu woreninepreventssolarultravioletinducedsunburnbyinhibitingjnk2 AT tengfeima woreninepreventssolarultravioletinducedsunburnbyinhibitingjnk2 AT xiaofangni woreninepreventssolarultravioletinducedsunburnbyinhibitingjnk2 AT tedingchang woreninepreventssolarultravioletinducedsunburnbyinhibitingjnk2 AT manliu woreninepreventssolarultravioletinducedsunburnbyinhibitingjnk2 AT nijieli woreninepreventssolarultravioletinducedsunburnbyinhibitingjnk2 AT peijianglu woreninepreventssolarultravioletinducedsunburnbyinhibitingjnk2 AT changshuke woreninepreventssolarultravioletinducedsunburnbyinhibitingjnk2 AT qintian woreninepreventssolarultravioletinducedsunburnbyinhibitingjnk2 AT lingzou woreninepreventssolarultravioletinducedsunburnbyinhibitingjnk2 AT feiwang woreninepreventssolarultravioletinducedsunburnbyinhibitingjnk2 AT weiwang woreninepreventssolarultravioletinducedsunburnbyinhibitingjnk2 AT luzhang woreninepreventssolarultravioletinducedsunburnbyinhibitingjnk2 AT pingyuan woreninepreventssolarultravioletinducedsunburnbyinhibitingjnk2 AT linliu woreninepreventssolarultravioletinducedsunburnbyinhibitingjnk2 AT jianminzhang woreninepreventssolarultravioletinducedsunburnbyinhibitingjnk2 AT feishi woreninepreventssolarultravioletinducedsunburnbyinhibitingjnk2 AT qiuhongduan woreninepreventssolarultravioletinducedsunburnbyinhibitingjnk2 AT fengzhu woreninepreventssolarultravioletinducedsunburnbyinhibitingjnk2 AT fengzhu woreninepreventssolarultravioletinducedsunburnbyinhibitingjnk2 AT fengzhu woreninepreventssolarultravioletinducedsunburnbyinhibitingjnk2 AT fengzhu woreninepreventssolarultravioletinducedsunburnbyinhibitingjnk2 |