Oxidative status of honey bees infected with Nosema ceranae microsporidium and supplemented with Agaricus bisporus mushroom extract

Nosema ceranae, a microsporidium species, is among the most common causes of bee diseases. The positive effect of Agaricus bisporus mushroom extract on the survival and immunity of Nosema-infected bees has been reported recently. The effect could be achieved by stimulating the expression of immune-r...

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Main Authors: Glavinić Uroš, Džogović Danica, Jelisić Stefan, Ristanić Marko, Zorc Minja, Aleksić Nevenka, Stanimirović Zoran
Format: Article
Language:English
Published: Faculty of Veterinary Medicine, Belgrade 2023-01-01
Series:Veterinarski Glasnik
Subjects:
Online Access:https://doiserbia.nb.rs/img/doi/0350-2457/2023/0350-24572200013G.pdf
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author Glavinić Uroš
Džogović Danica
Jelisić Stefan
Ristanić Marko
Zorc Minja
Aleksić Nevenka
Stanimirović Zoran
author_facet Glavinić Uroš
Džogović Danica
Jelisić Stefan
Ristanić Marko
Zorc Minja
Aleksić Nevenka
Stanimirović Zoran
author_sort Glavinić Uroš
collection DOAJ
description Nosema ceranae, a microsporidium species, is among the most common causes of bee diseases. The positive effect of Agaricus bisporus mushroom extract on the survival and immunity of Nosema-infected bees has been reported recently. The effect could be achieved by stimulating the expression of immune-related genes, but also by suppressing nosemosis. The aim of this work was to determine the effect of A. bisporus extract on the oxidative status of bees infected with N. ceranae. In a cage experiment on newly hatched bees, the effect of aqueous extract of champignon (A. bisporus, strain A15) was investigated. Six groups were formed: three groups were infected and received A. bisporus extract through food at different times (days 1, 3, and 6 after hatching), one group received the extract but was not infected (treatment control), one was only infected with Nosema (positive control) and one was neither infected nor received the extract (negative control). The effects were examined on samples taken on days 7 and 15 of the study. The activities of the antioxidant enzymes catalase (CAT), superoxide dismutase (SOD) and glutathione S-transferase (GST) and the concentrations of malondialdehyde (MDA) were determined. In comparison to the positive control, the enzyme activities and MDA concentrations were significantly lower in the groups fed with the mushroom extract supplement. In the negative control, the level of oxidative stress was lower than in the positive control. In comparison with the other groups, the values mostly did not differ significantly. The oxidative status of bees infected with N. ceranae was significantly better if they were fed with the A. bisporus extract.
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spelling doaj.art-2413dac016ad4627b37578188572b41c2023-06-09T10:44:35ZengFaculty of Veterinary Medicine, BelgradeVeterinarski Glasnik0350-24572406-07712023-01-01771355010.2298/VETGL220715013G0350-24572200013GOxidative status of honey bees infected with Nosema ceranae microsporidium and supplemented with Agaricus bisporus mushroom extractGlavinić Uroš0https://orcid.org/0000-0003-2143-1611Džogović Danica1Jelisić Stefan2https://orcid.org/0000-0002-4578-182XRistanić Marko3https://orcid.org/0000-0002-6084-7321Zorc Minja4https://orcid.org/0000-0003-3330-7909Aleksić Nevenka5https://orcid.org/0000-0003-2093-8656Stanimirović Zoran6https://orcid.org/0000-0003-2657-8591University of Belgrade, Faculty of Veterinary Medicine, Department of Biology, Belgrade, SerbiaUniversity of Belgrade, Faculty of Veterinary Medicine, Department of Biology, Belgrade, SerbiaUniversity of Belgrade, Faculty of Veterinary Medicine, Department of Biology, Belgrade, SerbiaUniversity of Belgrade, Faculty of Veterinary Medicine, Department of Biology, Belgrade, SerbiaUniversity of Ljubljana, Biotechnical Faculty, Department of Animal Science, Ljubljana, SloveniaUniversity of Belgrade, Faculty of Veterinary Medicine, Department of Parasitology, Belgrade, SerbiaUniversity of Belgrade, Faculty of Veterinary Medicine, Department of Biology, Belgrade, SerbiaNosema ceranae, a microsporidium species, is among the most common causes of bee diseases. The positive effect of Agaricus bisporus mushroom extract on the survival and immunity of Nosema-infected bees has been reported recently. The effect could be achieved by stimulating the expression of immune-related genes, but also by suppressing nosemosis. The aim of this work was to determine the effect of A. bisporus extract on the oxidative status of bees infected with N. ceranae. In a cage experiment on newly hatched bees, the effect of aqueous extract of champignon (A. bisporus, strain A15) was investigated. Six groups were formed: three groups were infected and received A. bisporus extract through food at different times (days 1, 3, and 6 after hatching), one group received the extract but was not infected (treatment control), one was only infected with Nosema (positive control) and one was neither infected nor received the extract (negative control). The effects were examined on samples taken on days 7 and 15 of the study. The activities of the antioxidant enzymes catalase (CAT), superoxide dismutase (SOD) and glutathione S-transferase (GST) and the concentrations of malondialdehyde (MDA) were determined. In comparison to the positive control, the enzyme activities and MDA concentrations were significantly lower in the groups fed with the mushroom extract supplement. In the negative control, the level of oxidative stress was lower than in the positive control. In comparison with the other groups, the values mostly did not differ significantly. The oxidative status of bees infected with N. ceranae was significantly better if they were fed with the A. bisporus extract.https://doiserbia.nb.rs/img/doi/0350-2457/2023/0350-24572200013G.pdfapis melliferanosema ceranaeagaricus bisporusoxidative stressoxidative status
spellingShingle Glavinić Uroš
Džogović Danica
Jelisić Stefan
Ristanić Marko
Zorc Minja
Aleksić Nevenka
Stanimirović Zoran
Oxidative status of honey bees infected with Nosema ceranae microsporidium and supplemented with Agaricus bisporus mushroom extract
Veterinarski Glasnik
apis mellifera
nosema ceranae
agaricus bisporus
oxidative stress
oxidative status
title Oxidative status of honey bees infected with Nosema ceranae microsporidium and supplemented with Agaricus bisporus mushroom extract
title_full Oxidative status of honey bees infected with Nosema ceranae microsporidium and supplemented with Agaricus bisporus mushroom extract
title_fullStr Oxidative status of honey bees infected with Nosema ceranae microsporidium and supplemented with Agaricus bisporus mushroom extract
title_full_unstemmed Oxidative status of honey bees infected with Nosema ceranae microsporidium and supplemented with Agaricus bisporus mushroom extract
title_short Oxidative status of honey bees infected with Nosema ceranae microsporidium and supplemented with Agaricus bisporus mushroom extract
title_sort oxidative status of honey bees infected with nosema ceranae microsporidium and supplemented with agaricus bisporus mushroom extract
topic apis mellifera
nosema ceranae
agaricus bisporus
oxidative stress
oxidative status
url https://doiserbia.nb.rs/img/doi/0350-2457/2023/0350-24572200013G.pdf
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