Repeated 28-Day Oral Toxicological Study and Gastroprotective Effects of <i>Nigella sativa</i> L. Oil (Shuhada) against Ethanol-Induced Gastric Mucosal Injury in Rats
<i>Nigella sativa</i> L. and black seeds are traditionally used for cooking and medicinal purposes in Arab and other countries. Although <i>N. sativa</i> seed extract has many known biological effects, the biological effects of cold-pressed <i>N. sativa</i> oil ar...
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MDPI AG
2023-03-01
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| author | Sineenart Sanpinit Palika Wetchakul Piriya Chonsut Ngamrayu Ngamdokmai Aktsar Roskiana Ahmad Sakan Warinhomhoun |
| author_facet | Sineenart Sanpinit Palika Wetchakul Piriya Chonsut Ngamrayu Ngamdokmai Aktsar Roskiana Ahmad Sakan Warinhomhoun |
| author_sort | Sineenart Sanpinit |
| collection | DOAJ |
| description | <i>Nigella sativa</i> L. and black seeds are traditionally used for cooking and medicinal purposes in Arab and other countries. Although <i>N. sativa</i> seed extract has many known biological effects, the biological effects of cold-pressed <i>N. sativa</i> oil are poorly understood. Therefore, the objective of this study was to investigate the gastroprotective effects and subacute oral toxicity of black seed oil (BSO) in an animal model. The gastroprotective effects of oral BSO (50% and 100%; 1 mg/kg) were tested using acute experimental models of ethanol-induced gastric ulcers. Gross and histological gastric lesions, ulcerated gastric areas, ulcer index score, percentage of inhibition rate, gastric juice pH, and gastric wall mucus were all evaluated. The subacute toxicity of BSO and its thymoquinone (TQ) content were also examined. The results indicated that the administration of BSO exerted gastroprotective effects by increasing the gastric wall mucus and decreasing gastric juice acidity. In the subacute toxicity test, the animals behaved normally, and their weight and water and food intake did not show significant variations. High-performance liquid chromatography detected 7.3 mg/mL TQ in BSO. These findings suggest that BSO may be a safe therapeutic drug for preventing gastric ulcers. |
| first_indexed | 2024-03-11T06:03:55Z |
| format | Article |
| id | doaj.art-2413ea0635b241789e52d6dc97078d9e |
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| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-11T06:03:55Z |
| publishDate | 2023-03-01 |
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| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-2413ea0635b241789e52d6dc97078d9e2023-11-17T13:07:06ZengMDPI AGNutrients2072-66432023-03-01156153210.3390/nu15061532Repeated 28-Day Oral Toxicological Study and Gastroprotective Effects of <i>Nigella sativa</i> L. Oil (Shuhada) against Ethanol-Induced Gastric Mucosal Injury in RatsSineenart Sanpinit0Palika Wetchakul1Piriya Chonsut2Ngamrayu Ngamdokmai3Aktsar Roskiana Ahmad4Sakan Warinhomhoun5Division of Applied Thai Traditional Medicine, School of Medicine, Walailak University, Nakhon Si Thammarat 80160, ThailandDivision of Applied Thai Traditional Medicine, School of Medicine, Walailak University, Nakhon Si Thammarat 80160, ThailandDivision of Applied Thai Traditional Medicine, School of Medicine, Walailak University, Nakhon Si Thammarat 80160, ThailandDivision of Applied Thai Traditional Medicine, School of Medicine, Walailak University, Nakhon Si Thammarat 80160, ThailandDepartment of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Universitas of Muslim Indonesia, Makassar 90241, South Sulawesi, IndonesiaDivision of Applied Thai Traditional Medicine, School of Medicine, Walailak University, Nakhon Si Thammarat 80160, Thailand<i>Nigella sativa</i> L. and black seeds are traditionally used for cooking and medicinal purposes in Arab and other countries. Although <i>N. sativa</i> seed extract has many known biological effects, the biological effects of cold-pressed <i>N. sativa</i> oil are poorly understood. Therefore, the objective of this study was to investigate the gastroprotective effects and subacute oral toxicity of black seed oil (BSO) in an animal model. The gastroprotective effects of oral BSO (50% and 100%; 1 mg/kg) were tested using acute experimental models of ethanol-induced gastric ulcers. Gross and histological gastric lesions, ulcerated gastric areas, ulcer index score, percentage of inhibition rate, gastric juice pH, and gastric wall mucus were all evaluated. The subacute toxicity of BSO and its thymoquinone (TQ) content were also examined. The results indicated that the administration of BSO exerted gastroprotective effects by increasing the gastric wall mucus and decreasing gastric juice acidity. In the subacute toxicity test, the animals behaved normally, and their weight and water and food intake did not show significant variations. High-performance liquid chromatography detected 7.3 mg/mL TQ in BSO. These findings suggest that BSO may be a safe therapeutic drug for preventing gastric ulcers.https://www.mdpi.com/2072-6643/15/6/1532traditional Arabic and Islamic medicineblack cumin seed oilthymoquinoneQurangastric ulcertoxicity |
| spellingShingle | Sineenart Sanpinit Palika Wetchakul Piriya Chonsut Ngamrayu Ngamdokmai Aktsar Roskiana Ahmad Sakan Warinhomhoun Repeated 28-Day Oral Toxicological Study and Gastroprotective Effects of <i>Nigella sativa</i> L. Oil (Shuhada) against Ethanol-Induced Gastric Mucosal Injury in Rats Nutrients traditional Arabic and Islamic medicine black cumin seed oil thymoquinone Quran gastric ulcer toxicity |
| title | Repeated 28-Day Oral Toxicological Study and Gastroprotective Effects of <i>Nigella sativa</i> L. Oil (Shuhada) against Ethanol-Induced Gastric Mucosal Injury in Rats |
| title_full | Repeated 28-Day Oral Toxicological Study and Gastroprotective Effects of <i>Nigella sativa</i> L. Oil (Shuhada) against Ethanol-Induced Gastric Mucosal Injury in Rats |
| title_fullStr | Repeated 28-Day Oral Toxicological Study and Gastroprotective Effects of <i>Nigella sativa</i> L. Oil (Shuhada) against Ethanol-Induced Gastric Mucosal Injury in Rats |
| title_full_unstemmed | Repeated 28-Day Oral Toxicological Study and Gastroprotective Effects of <i>Nigella sativa</i> L. Oil (Shuhada) against Ethanol-Induced Gastric Mucosal Injury in Rats |
| title_short | Repeated 28-Day Oral Toxicological Study and Gastroprotective Effects of <i>Nigella sativa</i> L. Oil (Shuhada) against Ethanol-Induced Gastric Mucosal Injury in Rats |
| title_sort | repeated 28 day oral toxicological study and gastroprotective effects of i nigella sativa i l oil shuhada against ethanol induced gastric mucosal injury in rats |
| topic | traditional Arabic and Islamic medicine black cumin seed oil thymoquinone Quran gastric ulcer toxicity |
| url | https://www.mdpi.com/2072-6643/15/6/1532 |
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