Analysis of Microbiota and Mycobiota in Fungal Ball Rhinosinusitis: Specific Interaction between <i>Aspergillus fumigatus</i> and <i>Haemophilus influenza</i>?
Fungal ball (FB) rhinosinusitis (RS) is the main type of non-invasive fungal RS. Despite positive direct examination (DE) of biopsies, culture remains negative in more than 60% of cases. The aim of the study was to evaluate the performance/efficacy of targeted metagenomics (TM) to analyze microbiota...
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MDPI AG
2021-07-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/7/7/550 |
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| author | Sarah Dellière Eric Dannaoui Maxime Fieux Pierre Bonfils Guillaume Gricourt Vanessa Demontant Isabelle Podglajen Paul-Louis Woerther Cécile Angebault Françoise Botterel |
| author_facet | Sarah Dellière Eric Dannaoui Maxime Fieux Pierre Bonfils Guillaume Gricourt Vanessa Demontant Isabelle Podglajen Paul-Louis Woerther Cécile Angebault Françoise Botterel |
| author_sort | Sarah Dellière |
| collection | DOAJ |
| description | Fungal ball (FB) rhinosinusitis (RS) is the main type of non-invasive fungal RS. Despite positive direct examination (DE) of biopsies, culture remains negative in more than 60% of cases. The aim of the study was to evaluate the performance/efficacy of targeted metagenomics (TM) to analyze microbiota and mycobiota in FB and find microbial associations. Forty-five sinus biopsies from patients who underwent surgery for chronic RS were included. After DE and culture, DNA was extracted, then fungal ITS1–ITS2 and bacterial V3–V4 16S rDNA loci were sequenced (MiSeq<sup>TM</sup> Illumina). Operational taxonomic units (OTUs) were defined via QIIME and assigned to SILVA (16S) and UNITE (ITS) databases. Statistical analyses were performed using SHAMAN. Thirty-eight patients had FB and seven had non-fungal rhinosinusitis (NFRS). DE and culture of FB were positive for fungi in 97.3 and 31.6% of patients, respectively. TM analysis of the 38 FB yielded more than one fungal genus in 100% of cases, with <i>Aspergillus</i> in 89.5% (34/38). <i>Haemophilus</i> was over-represented in FB with >1000 reads/sample in 47.3% (18/38) compared to NFRS (<i>p</i> < 0.001). TM allowed fungal identification in biopsies with negative culture. <i>Haemophilus</i> was associated with FB. Pathogenesis could result from fungi–bacteria interactions in a mixed biofilm-like structure. |
| first_indexed | 2024-03-10T09:35:04Z |
| format | Article |
| id | doaj.art-24153266644444bf8e0ef6e82157370b |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T09:35:04Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-24153266644444bf8e0ef6e82157370b2023-11-22T04:09:55ZengMDPI AGJournal of Fungi2309-608X2021-07-017755010.3390/jof7070550Analysis of Microbiota and Mycobiota in Fungal Ball Rhinosinusitis: Specific Interaction between <i>Aspergillus fumigatus</i> and <i>Haemophilus influenza</i>?Sarah Dellière0Eric Dannaoui1Maxime Fieux2Pierre Bonfils3Guillaume Gricourt4Vanessa Demontant5Isabelle Podglajen6Paul-Louis Woerther7Cécile Angebault8Françoise Botterel9Unité de Parasitologie-Mycologie, Département de Prévention, Diagnostic et Traitement des Infections, APHP, GHU Hôpitaux Universitaires Henri-Mondor, 94010 Créteil, FranceUR DYNAMiC 7380, Faculté de Santé, Université Paris-Est Créteil, 94010 Créteil, FranceDépartement d’Otorhinolaryngologie, Hôpital Européen George Pompidou, APHP, Université de Paris, 75015 Paris, FranceDépartement d’Otorhinolaryngologie, Hôpital Européen George Pompidou, APHP, Université de Paris, 75015 Paris, FrancePlate-Forme Genomiques, APHP-IMRB, GHU Hôpitaux Universitaires Henri-Mondor, UPEC, 94010 Créteil, FrancePlate-Forme Genomiques, APHP-IMRB, GHU Hôpitaux Universitaires Henri-Mondor, UPEC, 94010 Créteil, FranceUnité de Bactériologie, Département de Microbiologie, Hôpital Européen George Pompidou, APHP, Université de Paris, 75015 Paris, FranceUR DYNAMiC 7380, Faculté de Santé, Université Paris-Est Créteil, 94010 Créteil, FranceUnité de Parasitologie-Mycologie, Département de Prévention, Diagnostic et Traitement des Infections, APHP, GHU Hôpitaux Universitaires Henri-Mondor, 94010 Créteil, FranceUnité de Parasitologie-Mycologie, Département de Prévention, Diagnostic et Traitement des Infections, APHP, GHU Hôpitaux Universitaires Henri-Mondor, 94010 Créteil, FranceFungal ball (FB) rhinosinusitis (RS) is the main type of non-invasive fungal RS. Despite positive direct examination (DE) of biopsies, culture remains negative in more than 60% of cases. The aim of the study was to evaluate the performance/efficacy of targeted metagenomics (TM) to analyze microbiota and mycobiota in FB and find microbial associations. Forty-five sinus biopsies from patients who underwent surgery for chronic RS were included. After DE and culture, DNA was extracted, then fungal ITS1–ITS2 and bacterial V3–V4 16S rDNA loci were sequenced (MiSeq<sup>TM</sup> Illumina). Operational taxonomic units (OTUs) were defined via QIIME and assigned to SILVA (16S) and UNITE (ITS) databases. Statistical analyses were performed using SHAMAN. Thirty-eight patients had FB and seven had non-fungal rhinosinusitis (NFRS). DE and culture of FB were positive for fungi in 97.3 and 31.6% of patients, respectively. TM analysis of the 38 FB yielded more than one fungal genus in 100% of cases, with <i>Aspergillus</i> in 89.5% (34/38). <i>Haemophilus</i> was over-represented in FB with >1000 reads/sample in 47.3% (18/38) compared to NFRS (<i>p</i> < 0.001). TM allowed fungal identification in biopsies with negative culture. <i>Haemophilus</i> was associated with FB. Pathogenesis could result from fungi–bacteria interactions in a mixed biofilm-like structure.https://www.mdpi.com/2309-608X/7/7/550<i>Aspergillus fumigatus</i><i>Haemophilus influenzae</i>mycobiotamicrobiotamicrobial interactionsfungal–bacterial biofilm |
| spellingShingle | Sarah Dellière Eric Dannaoui Maxime Fieux Pierre Bonfils Guillaume Gricourt Vanessa Demontant Isabelle Podglajen Paul-Louis Woerther Cécile Angebault Françoise Botterel Analysis of Microbiota and Mycobiota in Fungal Ball Rhinosinusitis: Specific Interaction between <i>Aspergillus fumigatus</i> and <i>Haemophilus influenza</i>? Journal of Fungi <i>Aspergillus fumigatus</i> <i>Haemophilus influenzae</i> mycobiota microbiota microbial interactions fungal–bacterial biofilm |
| title | Analysis of Microbiota and Mycobiota in Fungal Ball Rhinosinusitis: Specific Interaction between <i>Aspergillus fumigatus</i> and <i>Haemophilus influenza</i>? |
| title_full | Analysis of Microbiota and Mycobiota in Fungal Ball Rhinosinusitis: Specific Interaction between <i>Aspergillus fumigatus</i> and <i>Haemophilus influenza</i>? |
| title_fullStr | Analysis of Microbiota and Mycobiota in Fungal Ball Rhinosinusitis: Specific Interaction between <i>Aspergillus fumigatus</i> and <i>Haemophilus influenza</i>? |
| title_full_unstemmed | Analysis of Microbiota and Mycobiota in Fungal Ball Rhinosinusitis: Specific Interaction between <i>Aspergillus fumigatus</i> and <i>Haemophilus influenza</i>? |
| title_short | Analysis of Microbiota and Mycobiota in Fungal Ball Rhinosinusitis: Specific Interaction between <i>Aspergillus fumigatus</i> and <i>Haemophilus influenza</i>? |
| title_sort | analysis of microbiota and mycobiota in fungal ball rhinosinusitis specific interaction between i aspergillus fumigatus i and i haemophilus influenza i |
| topic | <i>Aspergillus fumigatus</i> <i>Haemophilus influenzae</i> mycobiota microbiota microbial interactions fungal–bacterial biofilm |
| url | https://www.mdpi.com/2309-608X/7/7/550 |
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