Molecular characterisation of the current high prevalence of the new CPV‐2c variants in the Southern Vietnamese dogs signifies a widespread in the worldwide dog population
Abstract Background Canine parvovirus type 2 (CPV‐2) is known as the primary etiological agent cause of acute gastroenteritis, myocarditis and death of canids worldwide. In Vietnam, although CPV‐2 infection and its outbreaks are the most important risk factors of the canine's health concern, la...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-07-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.1163 |
| _version_ | 1797776569390858240 |
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| author | Thi To Nga Bui Minh Hoang Van Dung Nguyen Minh Nam Nguyen Van Thai Than |
| author_facet | Thi To Nga Bui Minh Hoang Van Dung Nguyen Minh Nam Nguyen Van Thai Than |
| author_sort | Thi To Nga Bui |
| collection | DOAJ |
| description | Abstract Background Canine parvovirus type 2 (CPV‐2) is known as the primary etiological agent cause of acute gastroenteritis, myocarditis and death of canids worldwide. In Vietnam, although CPV‐2 infection and its outbreaks are the most important risk factors of the canine's health concern, lack of available information about the molecular epidemiology of the CPV‐2. Objectives In this study, the complete coding sequences of 10 CPV‐2 strains collected from dogs vaccinated with CPV‐2 vaccination were analysed to better understand the genomic characteristics of the current circulating CPV‐2 in Vietnam. Methods Ten CPV‐specific PCR‐positive rectal swab samples were collected from dogs with acute symptoms of haemorrhagic diarrhoea and vomiting in Vietnam in 2019. The complete coding sequences of these CPV strains were analysed to determine their phylogeny and genetic relationship with other available CPV strains globally. Results Analysis of the VP2 gene sequences demonstrated that the studied strains belonged to the new CPV‐2c variants with the unique mutations at amino acids 5Ala‐Gly and 447Iso‐Met. Phylogenetic tree analysis indicated that the studied strains share a common evolutionary origin with the current CPV‐2c strains circulating in dogs in Asia countries, including China, Thailand, Taiwan and Mongolia, in recent years. Low sequence identity between the studied strains and commercial vaccine strains was observed. Conclusions This study provides deep insights into the molecular characteristics, genetic diversity, and evolution of circulating CPV‐2 strains in Vietnam. We recommend more studies to estimate the effectiveness of the CPV vaccine and the need to continue developing other effective vaccination essential to better control the widespread of these new CPV‐2 variants. |
| first_indexed | 2024-03-12T22:51:46Z |
| format | Article |
| id | doaj.art-241ac804c17b4434887fdd059ea9a867 |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-03-12T22:51:46Z |
| publishDate | 2023-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-241ac804c17b4434887fdd059ea9a8672023-07-20T08:09:46ZengWileyVeterinary Medicine and Science2053-10952023-07-01941553156310.1002/vms3.1163Molecular characterisation of the current high prevalence of the new CPV‐2c variants in the Southern Vietnamese dogs signifies a widespread in the worldwide dog populationThi To Nga Bui0Minh Hoang1Van Dung Nguyen2Minh Nam Nguyen3Van Thai Than4Department of Veterinary Pathology, College of Veterinary Medicine Vietnam National University of Agriculture Hanoi VietnamDepartment of Veterinary Pathology, College of Veterinary Medicine Vietnam National University of Agriculture Hanoi VietnamSub‐Department of Animal Health of Ho Chi Minh City Ho Chi Minh City VietnamResearch Center for Genetics and Reproductive Health School of Medicine Vietnam National University Ho Chi Minh City Ho Chi Minh City VietnamFaculty of Applied Sciences International School Vietnam National University Hanoi VietnamAbstract Background Canine parvovirus type 2 (CPV‐2) is known as the primary etiological agent cause of acute gastroenteritis, myocarditis and death of canids worldwide. In Vietnam, although CPV‐2 infection and its outbreaks are the most important risk factors of the canine's health concern, lack of available information about the molecular epidemiology of the CPV‐2. Objectives In this study, the complete coding sequences of 10 CPV‐2 strains collected from dogs vaccinated with CPV‐2 vaccination were analysed to better understand the genomic characteristics of the current circulating CPV‐2 in Vietnam. Methods Ten CPV‐specific PCR‐positive rectal swab samples were collected from dogs with acute symptoms of haemorrhagic diarrhoea and vomiting in Vietnam in 2019. The complete coding sequences of these CPV strains were analysed to determine their phylogeny and genetic relationship with other available CPV strains globally. Results Analysis of the VP2 gene sequences demonstrated that the studied strains belonged to the new CPV‐2c variants with the unique mutations at amino acids 5Ala‐Gly and 447Iso‐Met. Phylogenetic tree analysis indicated that the studied strains share a common evolutionary origin with the current CPV‐2c strains circulating in dogs in Asia countries, including China, Thailand, Taiwan and Mongolia, in recent years. Low sequence identity between the studied strains and commercial vaccine strains was observed. Conclusions This study provides deep insights into the molecular characteristics, genetic diversity, and evolution of circulating CPV‐2 strains in Vietnam. We recommend more studies to estimate the effectiveness of the CPV vaccine and the need to continue developing other effective vaccination essential to better control the widespread of these new CPV‐2 variants.https://doi.org/10.1002/vms3.1163canine parvovirusgenome characterisationnew CPV‐2c variantVietnam |
| spellingShingle | Thi To Nga Bui Minh Hoang Van Dung Nguyen Minh Nam Nguyen Van Thai Than Molecular characterisation of the current high prevalence of the new CPV‐2c variants in the Southern Vietnamese dogs signifies a widespread in the worldwide dog population Veterinary Medicine and Science canine parvovirus genome characterisation new CPV‐2c variant Vietnam |
| title | Molecular characterisation of the current high prevalence of the new CPV‐2c variants in the Southern Vietnamese dogs signifies a widespread in the worldwide dog population |
| title_full | Molecular characterisation of the current high prevalence of the new CPV‐2c variants in the Southern Vietnamese dogs signifies a widespread in the worldwide dog population |
| title_fullStr | Molecular characterisation of the current high prevalence of the new CPV‐2c variants in the Southern Vietnamese dogs signifies a widespread in the worldwide dog population |
| title_full_unstemmed | Molecular characterisation of the current high prevalence of the new CPV‐2c variants in the Southern Vietnamese dogs signifies a widespread in the worldwide dog population |
| title_short | Molecular characterisation of the current high prevalence of the new CPV‐2c variants in the Southern Vietnamese dogs signifies a widespread in the worldwide dog population |
| title_sort | molecular characterisation of the current high prevalence of the new cpv 2c variants in the southern vietnamese dogs signifies a widespread in the worldwide dog population |
| topic | canine parvovirus genome characterisation new CPV‐2c variant Vietnam |
| url | https://doi.org/10.1002/vms3.1163 |
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