Effect of PEA-OXA on neuropathic pain and functional recovery after sciatic nerve crush
Abstract Background Animal models of sciatic nerve injury are commonly used to study neuropathic pain as well as axon regeneration. Inflammation/immune response at the site of nerve lesion is known to be an essential trigger of the pathological changes that have a critical impact on nerve repair and...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-09-01
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| Series: | Journal of Neuroinflammation |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12974-018-1303-5 |
| _version_ | 1819076000514834432 |
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| author | Enrico Gugliandolo Ramona D’amico Marika Cordaro Roberta Fusco Rosalba Siracusa Rosalia Crupi Daniela Impellizzeri Salvatore Cuzzocrea Rosanna Di Paola |
| author_facet | Enrico Gugliandolo Ramona D’amico Marika Cordaro Roberta Fusco Rosalba Siracusa Rosalia Crupi Daniela Impellizzeri Salvatore Cuzzocrea Rosanna Di Paola |
| author_sort | Enrico Gugliandolo |
| collection | DOAJ |
| description | Abstract Background Animal models of sciatic nerve injury are commonly used to study neuropathic pain as well as axon regeneration. Inflammation/immune response at the site of nerve lesion is known to be an essential trigger of the pathological changes that have a critical impact on nerve repair and regeneration; moreover, the damage to peripheral nerve can cause a loss of sensory function and produces a persistent neuropathic pain. N-Acylethanolamines (NAEs) involve a family of lipid molecules existent in animal and plant, of which is N-palmitoylethanolamide (PEA) that arouses great attention owing to its anti-inflammatory, analgesic, and neuroprotective activities. The modulation of specific amidases for NAEs (and in particular NAE-hydrolyzing acid amidase NAAA, which is more selective for PEA) could be a condition to preserve its levels. Here, we investigated, in a mice model of sciatic nerve crush, the effect of 2-pentadecyl-2-oxazoline (PEA-OXA) the oxazoline of PEA that reportedly modulates activity of NAAA. Methods In this experimental model, the mice, following the sciatic nerve crush, were treated daily with PEA-OXA at a dose of 10 mg\kg for 14 days. Therefore, we evaluated the effects of PEA-OXA on the degree of injury, on the inhibition of neuropathic pain, and on the inflammatory process, as in the improvement of reparative processes and therefore in the restoration of locomotor function. Results Our results showed that PEA-OXA (10 mg/kg) treatment, daily, for 14 days after sciatic nerve crush, have an anti-inflammatory and neuroprotective effect and moreover have an analgesic protective effect on hypersensitivity, and improve the functional recovery after nerve crush. Conclusions Therefore, treatment with PEA-OXA as a whole has shown a protective effect, which makes it a powerful candidate for the treatment of peripheral nerve injury and neuropathic pain. |
| first_indexed | 2024-12-21T18:34:21Z |
| format | Article |
| id | doaj.art-241bbd2fd47d4afcb6ddefd84b4bc679 |
| institution | Directory Open Access Journal |
| issn | 1742-2094 |
| language | English |
| last_indexed | 2024-12-21T18:34:21Z |
| publishDate | 2018-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Neuroinflammation |
| spelling | doaj.art-241bbd2fd47d4afcb6ddefd84b4bc6792022-12-21T18:54:11ZengBMCJournal of Neuroinflammation1742-20942018-09-0115111310.1186/s12974-018-1303-5Effect of PEA-OXA on neuropathic pain and functional recovery after sciatic nerve crushEnrico Gugliandolo0Ramona D’amico1Marika Cordaro2Roberta Fusco3Rosalba Siracusa4Rosalia Crupi5Daniela Impellizzeri6Salvatore Cuzzocrea7Rosanna Di Paola8Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno D’Alcontres, no 31Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno D’Alcontres, no 31Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno D’Alcontres, no 31Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno D’Alcontres, no 31Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno D’Alcontres, no 31Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno D’Alcontres, no 31Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno D’Alcontres, no 31Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno D’Alcontres, no 31Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno D’Alcontres, no 31Abstract Background Animal models of sciatic nerve injury are commonly used to study neuropathic pain as well as axon regeneration. Inflammation/immune response at the site of nerve lesion is known to be an essential trigger of the pathological changes that have a critical impact on nerve repair and regeneration; moreover, the damage to peripheral nerve can cause a loss of sensory function and produces a persistent neuropathic pain. N-Acylethanolamines (NAEs) involve a family of lipid molecules existent in animal and plant, of which is N-palmitoylethanolamide (PEA) that arouses great attention owing to its anti-inflammatory, analgesic, and neuroprotective activities. The modulation of specific amidases for NAEs (and in particular NAE-hydrolyzing acid amidase NAAA, which is more selective for PEA) could be a condition to preserve its levels. Here, we investigated, in a mice model of sciatic nerve crush, the effect of 2-pentadecyl-2-oxazoline (PEA-OXA) the oxazoline of PEA that reportedly modulates activity of NAAA. Methods In this experimental model, the mice, following the sciatic nerve crush, were treated daily with PEA-OXA at a dose of 10 mg\kg for 14 days. Therefore, we evaluated the effects of PEA-OXA on the degree of injury, on the inhibition of neuropathic pain, and on the inflammatory process, as in the improvement of reparative processes and therefore in the restoration of locomotor function. Results Our results showed that PEA-OXA (10 mg/kg) treatment, daily, for 14 days after sciatic nerve crush, have an anti-inflammatory and neuroprotective effect and moreover have an analgesic protective effect on hypersensitivity, and improve the functional recovery after nerve crush. Conclusions Therefore, treatment with PEA-OXA as a whole has shown a protective effect, which makes it a powerful candidate for the treatment of peripheral nerve injury and neuropathic pain.http://link.springer.com/article/10.1186/s12974-018-1303-5NeuroinflammationPEA-OXASciatic nerve |
| spellingShingle | Enrico Gugliandolo Ramona D’amico Marika Cordaro Roberta Fusco Rosalba Siracusa Rosalia Crupi Daniela Impellizzeri Salvatore Cuzzocrea Rosanna Di Paola Effect of PEA-OXA on neuropathic pain and functional recovery after sciatic nerve crush Journal of Neuroinflammation Neuroinflammation PEA-OXA Sciatic nerve |
| title | Effect of PEA-OXA on neuropathic pain and functional recovery after sciatic nerve crush |
| title_full | Effect of PEA-OXA on neuropathic pain and functional recovery after sciatic nerve crush |
| title_fullStr | Effect of PEA-OXA on neuropathic pain and functional recovery after sciatic nerve crush |
| title_full_unstemmed | Effect of PEA-OXA on neuropathic pain and functional recovery after sciatic nerve crush |
| title_short | Effect of PEA-OXA on neuropathic pain and functional recovery after sciatic nerve crush |
| title_sort | effect of pea oxa on neuropathic pain and functional recovery after sciatic nerve crush |
| topic | Neuroinflammation PEA-OXA Sciatic nerve |
| url | http://link.springer.com/article/10.1186/s12974-018-1303-5 |
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