Shaping the Cell and the Future: Recent Advancements in Biophysical Aspects Relevant to Regenerative Medicine
In a worldwide effort to generate clinically useful therapeutic or preventive interventions, harnessing biophysical stimuli for directing cell fate is a powerful strategy. With the vision to control cell function through engineering cell shape, better understanding, measuring, and controlling cell s...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-12-01
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| Series: | Journal of Functional Morphology and Kinesiology |
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| Online Access: | https://www.mdpi.com/2411-5142/3/1/2 |
| _version_ | 1819099886873739264 |
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| author | Melanie L. Hart Jasmin C. Lauer Mischa Selig Martha Hanak Brandan Walters Bernd Rolauffs |
| author_facet | Melanie L. Hart Jasmin C. Lauer Mischa Selig Martha Hanak Brandan Walters Bernd Rolauffs |
| author_sort | Melanie L. Hart |
| collection | DOAJ |
| description | In a worldwide effort to generate clinically useful therapeutic or preventive interventions, harnessing biophysical stimuli for directing cell fate is a powerful strategy. With the vision to control cell function through engineering cell shape, better understanding, measuring, and controlling cell shape for ultimately utilizing cell shape-instructive materials is an emerging “hot” topic in regenerative medicine. This review highlights how quantitation of cellular morphology is useful not only for understanding the effects of different microenvironmental or biophysical stimuli on cells, but also how it could be used as a predictive marker of biological responses, e.g., by predicting future mesenchymal stromal cell differentiation. We introduce how high throughput image analysis, combined with computational tools, are increasingly being used to efficiently and accurately recognize cells. Moreover, we discuss how a panel of quantitative shape descriptors may be useful for measuring specific aspects of cellular and nuclear morphology in cell culture and tissues. This review focuses on the mechano-biological principle(s) through which biophysical cues can affect cellular shape, and recent insights on how specific cellular “baseline shapes” can intentionally be engineered, using biophysical cues. Hence, this review hopes to reveal how measuring and controlling cellular shape may aid in future regenerative medicine applications. |
| first_indexed | 2024-12-22T00:54:01Z |
| format | Article |
| id | doaj.art-241c115c15fd47d59a8b015069b8a4da |
| institution | Directory Open Access Journal |
| issn | 2411-5142 |
| language | English |
| last_indexed | 2024-12-22T00:54:01Z |
| publishDate | 2017-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Morphology and Kinesiology |
| spelling | doaj.art-241c115c15fd47d59a8b015069b8a4da2022-12-21T18:44:22ZengMDPI AGJournal of Functional Morphology and Kinesiology2411-51422017-12-0131210.3390/jfmk3010002jfmk3010002Shaping the Cell and the Future: Recent Advancements in Biophysical Aspects Relevant to Regenerative MedicineMelanie L. Hart0Jasmin C. Lauer1Mischa Selig2Martha Hanak3Brandan Walters4Bernd Rolauffs5G.E.R.N. Tissue Replacement, Regeneration & Neogenesis, Department of Orthopedics and Trauma Surgery, Medical Center—Albert-Ludwigs-University of Freiburg, Faculty of Medicine, Albert-Ludwigs-University of Freiburg, Hugstetter Straße 55, 79106 Freiburg, GermanyG.E.R.N. Tissue Replacement, Regeneration & Neogenesis, Department of Orthopedics and Trauma Surgery, Medical Center—Albert-Ludwigs-University of Freiburg, Faculty of Medicine, Albert-Ludwigs-University of Freiburg, Hugstetter Straße 55, 79106 Freiburg, GermanyG.E.R.N. Tissue Replacement, Regeneration & Neogenesis, Department of Orthopedics and Trauma Surgery, Medical Center—Albert-Ludwigs-University of Freiburg, Faculty of Medicine, Albert-Ludwigs-University of Freiburg, Hugstetter Straße 55, 79106 Freiburg, GermanyG.E.R.N. Tissue Replacement, Regeneration & Neogenesis, Department of Orthopedics and Trauma Surgery, Medical Center—Albert-Ludwigs-University of Freiburg, Faculty of Medicine, Albert-Ludwigs-University of Freiburg, Hugstetter Straße 55, 79106 Freiburg, GermanyDepartment of Biomedical Engineering, University of Michigan, Michigan, MI 48109, USAG.E.R.N. Tissue Replacement, Regeneration & Neogenesis, Department of Orthopedics and Trauma Surgery, Medical Center—Albert-Ludwigs-University of Freiburg, Faculty of Medicine, Albert-Ludwigs-University of Freiburg, Hugstetter Straße 55, 79106 Freiburg, GermanyIn a worldwide effort to generate clinically useful therapeutic or preventive interventions, harnessing biophysical stimuli for directing cell fate is a powerful strategy. With the vision to control cell function through engineering cell shape, better understanding, measuring, and controlling cell shape for ultimately utilizing cell shape-instructive materials is an emerging “hot” topic in regenerative medicine. This review highlights how quantitation of cellular morphology is useful not only for understanding the effects of different microenvironmental or biophysical stimuli on cells, but also how it could be used as a predictive marker of biological responses, e.g., by predicting future mesenchymal stromal cell differentiation. We introduce how high throughput image analysis, combined with computational tools, are increasingly being used to efficiently and accurately recognize cells. Moreover, we discuss how a panel of quantitative shape descriptors may be useful for measuring specific aspects of cellular and nuclear morphology in cell culture and tissues. This review focuses on the mechano-biological principle(s) through which biophysical cues can affect cellular shape, and recent insights on how specific cellular “baseline shapes” can intentionally be engineered, using biophysical cues. Hence, this review hopes to reveal how measuring and controlling cellular shape may aid in future regenerative medicine applications.https://www.mdpi.com/2411-5142/3/1/2cell morphologycell shapebiophysical cuestissue engineeringcell imagingquantitative analysisengineering cell morphologypredicting phenotypic outcomesmorphological signaturesmorphological fingerprintsmesenchymal stromal cells |
| spellingShingle | Melanie L. Hart Jasmin C. Lauer Mischa Selig Martha Hanak Brandan Walters Bernd Rolauffs Shaping the Cell and the Future: Recent Advancements in Biophysical Aspects Relevant to Regenerative Medicine Journal of Functional Morphology and Kinesiology cell morphology cell shape biophysical cues tissue engineering cell imaging quantitative analysis engineering cell morphology predicting phenotypic outcomes morphological signatures morphological fingerprints mesenchymal stromal cells |
| title | Shaping the Cell and the Future: Recent Advancements in Biophysical Aspects Relevant to Regenerative Medicine |
| title_full | Shaping the Cell and the Future: Recent Advancements in Biophysical Aspects Relevant to Regenerative Medicine |
| title_fullStr | Shaping the Cell and the Future: Recent Advancements in Biophysical Aspects Relevant to Regenerative Medicine |
| title_full_unstemmed | Shaping the Cell and the Future: Recent Advancements in Biophysical Aspects Relevant to Regenerative Medicine |
| title_short | Shaping the Cell and the Future: Recent Advancements in Biophysical Aspects Relevant to Regenerative Medicine |
| title_sort | shaping the cell and the future recent advancements in biophysical aspects relevant to regenerative medicine |
| topic | cell morphology cell shape biophysical cues tissue engineering cell imaging quantitative analysis engineering cell morphology predicting phenotypic outcomes morphological signatures morphological fingerprints mesenchymal stromal cells |
| url | https://www.mdpi.com/2411-5142/3/1/2 |
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