Distribution of campylobacter species in different water sources in parts of Enugu State of Nigeria

Aim: To determine the prevalence and possible reservoirs of Campylobacter species in water samples from different sources in Enugu and Nsukka areas of Enugu State, Nigeria. Methods: Four hundred and eighty (480) samples were obtained from the different water sources, using sterile bacteriological sa...

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Bibliographic Details
Main Authors: N F Onyemelukwe, Marcus Akaolisa
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2001-01-01
Series:International Journal of Medicine and Health Development
Subjects:
Online Access:http://www.ijmhdev.com/article.asp?issn=2635-3695;year=2001;volume=6;issue=1;spage=57;epage=59;aulast=Onyemelukwe;type=0
Description
Summary:Aim: To determine the prevalence and possible reservoirs of Campylobacter species in water samples from different sources in Enugu and Nsukka areas of Enugu State, Nigeria. Methods: Four hundred and eighty (480) samples were obtained from the different water sources, using sterile bacteriological samples bottles following standard protocols. The samples were maintained at between 8 and 15°C and processed within 1 hour of collection. Examination for Campylobacter species was accordingly carried out using a membrane filtration procedure (Celman CN - 6 metrical 47mm/0.45un membrane fitters) using 300ml samples volumes per sources. Butzlers' selective medium was used for isolating and incubation was done by the candle jar method at 42°C. Biochemical characterization of the strains was carried out and species identified by standard micirobiologkal procedures. Results: 146 (34.2%) samples contained Campylobacter spp. Speciation shows the following prevalent rate of isolates: C: jejuni 97 (59.1%), C. Coli 48(29.3%), C. Lardis 14 (8.5%) and C. Faecalis 5 (3.0%). Stagnant waters were most highly contaminated followed by well waters. Tap water was the least contaminated. Conclusions: Water is a reservoir for Campylobacter species and the risks inhabitants are exposed to through these waters are highlighted.
ISSN:2635-3695