Best Practices and Progress in Precision-Cut Liver Slice Cultures
Thirty-five years ago, precision-cut liver slices (PCLS) were described as a promising tool and were expected to become the standard in vitro model to study liver disease as they tick off all characteristics of a good in vitro model. In contrast to most in vitro models, PCLS retain the complex 3D li...
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Format: | Article |
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MDPI AG
2021-07-01
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Series: | International Journal of Molecular Sciences |
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Online Access: | https://www.mdpi.com/1422-0067/22/13/7137 |
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author | Liza Dewyse Hendrik Reynaert Leo A. van Grunsven |
author_facet | Liza Dewyse Hendrik Reynaert Leo A. van Grunsven |
author_sort | Liza Dewyse |
collection | DOAJ |
description | Thirty-five years ago, precision-cut liver slices (PCLS) were described as a promising tool and were expected to become the standard in vitro model to study liver disease as they tick off all characteristics of a good in vitro model. In contrast to most in vitro models, PCLS retain the complex 3D liver structures found in vivo, including cell–cell and cell–matrix interactions, and therefore should constitute the most reliable tool to model and to investigate pathways underlying chronic liver disease in vitro. Nevertheless, the biggest disadvantage of the model is the initiation of a procedure-induced fibrotic response. In this review, we describe the parameters and potential of PCLS cultures and discuss whether the initially described limitations and pitfalls have been overcome. We summarize the latest advances in PCLS research and critically evaluate PCLS use and progress since its invention in 1985. |
first_indexed | 2024-03-10T09:51:33Z |
format | Article |
id | doaj.art-24214b2a23df4765a152c9f16ae8276c |
institution | Directory Open Access Journal |
issn | 1661-6596 1422-0067 |
language | English |
last_indexed | 2024-03-10T09:51:33Z |
publishDate | 2021-07-01 |
publisher | MDPI AG |
record_format | Article |
series | International Journal of Molecular Sciences |
spelling | doaj.art-24214b2a23df4765a152c9f16ae8276c2023-11-22T02:40:46ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-07-012213713710.3390/ijms22137137Best Practices and Progress in Precision-Cut Liver Slice CulturesLiza Dewyse0Hendrik Reynaert1Leo A. van Grunsven2Liver Cell Biology Research Group, Department of Basic Biomedical Sciences, Vrije Universiteit Brussel, 1090 Brussels, BelgiumDepartment of Gastro-Enterology and Hepatology, Universitair Ziekenhuis Brussel, 1090 Brussels, BelgiumLiver Cell Biology Research Group, Department of Basic Biomedical Sciences, Vrije Universiteit Brussel, 1090 Brussels, BelgiumThirty-five years ago, precision-cut liver slices (PCLS) were described as a promising tool and were expected to become the standard in vitro model to study liver disease as they tick off all characteristics of a good in vitro model. In contrast to most in vitro models, PCLS retain the complex 3D liver structures found in vivo, including cell–cell and cell–matrix interactions, and therefore should constitute the most reliable tool to model and to investigate pathways underlying chronic liver disease in vitro. Nevertheless, the biggest disadvantage of the model is the initiation of a procedure-induced fibrotic response. In this review, we describe the parameters and potential of PCLS cultures and discuss whether the initially described limitations and pitfalls have been overcome. We summarize the latest advances in PCLS research and critically evaluate PCLS use and progress since its invention in 1985.https://www.mdpi.com/1422-0067/22/13/7137PCLSchronic liver disease3Din vitro liver |
spellingShingle | Liza Dewyse Hendrik Reynaert Leo A. van Grunsven Best Practices and Progress in Precision-Cut Liver Slice Cultures International Journal of Molecular Sciences PCLS chronic liver disease 3D in vitro liver |
title | Best Practices and Progress in Precision-Cut Liver Slice Cultures |
title_full | Best Practices and Progress in Precision-Cut Liver Slice Cultures |
title_fullStr | Best Practices and Progress in Precision-Cut Liver Slice Cultures |
title_full_unstemmed | Best Practices and Progress in Precision-Cut Liver Slice Cultures |
title_short | Best Practices and Progress in Precision-Cut Liver Slice Cultures |
title_sort | best practices and progress in precision cut liver slice cultures |
topic | PCLS chronic liver disease 3D in vitro liver |
url | https://www.mdpi.com/1422-0067/22/13/7137 |
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