The TRACE-Seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cells
Genetic barcoding has been used to track clonal dynamics of cells. Here, the authors develop a Tracking Recombination Alleles in Clonal Engraftment using sequencing (TRACE-Seq), to barcode repaired alleles by introducing silent mutations or outside of coding regions, to show clonal complexity of edi...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-20792-y |
| _version_ | 1818692140492914688 |
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| author | Rajiv Sharma Daniel P. Dever Ciaran M. Lee Armon Azizi Yidan Pan Joab Camarena Thomas Köhnke Gang Bao Matthew H. Porteus Ravindra Majeti |
| author_facet | Rajiv Sharma Daniel P. Dever Ciaran M. Lee Armon Azizi Yidan Pan Joab Camarena Thomas Köhnke Gang Bao Matthew H. Porteus Ravindra Majeti |
| author_sort | Rajiv Sharma |
| collection | DOAJ |
| description | Genetic barcoding has been used to track clonal dynamics of cells. Here, the authors develop a Tracking Recombination Alleles in Clonal Engraftment using sequencing (TRACE-Seq), to barcode repaired alleles by introducing silent mutations or outside of coding regions, to show clonal complexity of edited CD34 + cells following engraftment. |
| first_indexed | 2024-12-17T12:53:03Z |
| format | Article |
| id | doaj.art-24227f1bebe84818bfcc9151aa481b5d |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-17T12:53:03Z |
| publishDate | 2021-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-24227f1bebe84818bfcc9151aa481b5d2022-12-21T21:47:32ZengNature PortfolioNature Communications2041-17232021-01-0112111210.1038/s41467-020-20792-yThe TRACE-Seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cellsRajiv Sharma0Daniel P. Dever1Ciaran M. Lee2Armon Azizi3Yidan Pan4Joab Camarena5Thomas Köhnke6Gang Bao7Matthew H. Porteus8Ravindra Majeti9Department of Medicine, Division of Hematology, Cancer Institute, and Institute for Stem Cell Biology and Regenerative Medicine, Stanford UniversityDepartment of Pediatrics, Stanford UniversityDepartment of Bioengineering, Rice UniversityDepartment of Medicine, Division of Hematology, Cancer Institute, and Institute for Stem Cell Biology and Regenerative Medicine, Stanford UniversityDepartment of Bioengineering, Rice UniversityDepartment of Pediatrics, Stanford UniversityDepartment of Medicine, Division of Hematology, Cancer Institute, and Institute for Stem Cell Biology and Regenerative Medicine, Stanford UniversityDepartment of Bioengineering, Rice UniversityDepartment of Pediatrics, Stanford UniversityDepartment of Medicine, Division of Hematology, Cancer Institute, and Institute for Stem Cell Biology and Regenerative Medicine, Stanford UniversityGenetic barcoding has been used to track clonal dynamics of cells. Here, the authors develop a Tracking Recombination Alleles in Clonal Engraftment using sequencing (TRACE-Seq), to barcode repaired alleles by introducing silent mutations or outside of coding regions, to show clonal complexity of edited CD34 + cells following engraftment.https://doi.org/10.1038/s41467-020-20792-y |
| spellingShingle | Rajiv Sharma Daniel P. Dever Ciaran M. Lee Armon Azizi Yidan Pan Joab Camarena Thomas Köhnke Gang Bao Matthew H. Porteus Ravindra Majeti The TRACE-Seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cells Nature Communications |
| title | The TRACE-Seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cells |
| title_full | The TRACE-Seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cells |
| title_fullStr | The TRACE-Seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cells |
| title_full_unstemmed | The TRACE-Seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cells |
| title_short | The TRACE-Seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cells |
| title_sort | trace seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cells |
| url | https://doi.org/10.1038/s41467-020-20792-y |
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