Quantitative trait loci for resistance to trichostrongylid infection in Spanish Churra sheep
<p>Abstract</p> <p>Background</p> <p>For ruminants reared on grazing systems, gastrointestinal nematode (GIN) parasite infections represent the class of diseases with the greatest impact on animal health and productivity. Among the many possible strategies for controlli...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | deu |
| Published: |
BMC
2009-10-01
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| Series: | Genetics Selection Evolution |
| Online Access: | http://www.gsejournal.org/content/41/1/46 |
| _version_ | 1818084207509897216 |
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| author | Primitivo Fermin San Meana Aranzazu Bayón Yolanda de la Fuente Luis-Fernando Martínez-Valladares Maria Álvarez Lorena Pérez Jorge Gutiérrez-Gil Beatriz Rojo-Vázquez Francisco-Antonio Arranz Juan-José |
| author_facet | Primitivo Fermin San Meana Aranzazu Bayón Yolanda de la Fuente Luis-Fernando Martínez-Valladares Maria Álvarez Lorena Pérez Jorge Gutiérrez-Gil Beatriz Rojo-Vázquez Francisco-Antonio Arranz Juan-José |
| author_sort | Primitivo Fermin San |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>For ruminants reared on grazing systems, gastrointestinal nematode (GIN) parasite infections represent the class of diseases with the greatest impact on animal health and productivity. Among the many possible strategies for controlling GIN infection, the enhancement of host resistance through the selection of resistant animals has been suggested by many authors. Because of the difficulty of routinely collecting phenotypic indicators of parasite resistance, information derived from molecular markers may be used to improve the efficiency of classical genetic breeding.</p> <p>Methods</p> <p>A total of 181 microsatellite markers evenly distributed along the 26 sheep autosomes were used in a genome scan analysis performed in a commercial population of Spanish Churra sheep to detect chromosomal regions associated with parasite resistance. Following a daughter design, we analysed 322 ewes distributed in eight half-sib families. The phenotypes studied included two faecal egg counts (<it>LFEC0 </it>and <it>LFEC1</it>), anti-<it>Teladorsagia circumcincta </it>LIV IgA levels (<it>IgA</it>) and serum pepsinogen levels (<it>Peps</it>).</p> <p>Results</p> <p>The regression analysis revealed one QTL at the 5% genome-wise significance level on chromosome 6 for <it>LFEC1 </it>within the marker interval <it>BM4621-CSN3</it>. This QTL was found to be segregating in three out of the eight families analysed. Four other QTL were identified at the 5% chromosome-wise level on chromosomes 1, 10 and 14. Three of these QTL influenced faecal egg count, and the other one had an effect on <it>IgA </it>levels.</p> <p>Conclusion</p> <p>This study has successfully identified segregating QTL for parasite resistance traits in a commercial population. For some of the QTL detected, we have identified interesting coincidences with QTL previously reported in sheep, although most of those studies have been focused on young animals. Some of these coincidences might indicate that some common underlying loci affect parasite resistance traits in different sheep breeds. The identification of new QTL may suggest the existence of complex host-parasite relationships that have unique features depending on the host-parasite combination, perhaps due to the different mechanisms underlying resistance in adult sheep (hypersensitivity reactions) and lambs (immunity). The most significant QTL identified on chromosome 6 for <it>LFEC</it><sub>1 </sub>may be the target for future fine-mapping research efforts.</p> |
| first_indexed | 2024-12-10T19:50:13Z |
| format | Article |
| id | doaj.art-2424906561fd4511a392f5d91ec63a17 |
| institution | Directory Open Access Journal |
| issn | 0999-193X 1297-9686 |
| language | deu |
| last_indexed | 2024-12-10T19:50:13Z |
| publishDate | 2009-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Genetics Selection Evolution |
| spelling | doaj.art-2424906561fd4511a392f5d91ec63a172022-12-22T01:35:48ZdeuBMCGenetics Selection Evolution0999-193X1297-96862009-10-014114610.1186/1297-9686-41-46Quantitative trait loci for resistance to trichostrongylid infection in Spanish Churra sheepPrimitivo Fermin SanMeana AranzazuBayón Yolandade la Fuente Luis-FernandoMartínez-Valladares MariaÁlvarez LorenaPérez JorgeGutiérrez-Gil BeatrizRojo-Vázquez Francisco-AntonioArranz Juan-José<p>Abstract</p> <p>Background</p> <p>For ruminants reared on grazing systems, gastrointestinal nematode (GIN) parasite infections represent the class of diseases with the greatest impact on animal health and productivity. Among the many possible strategies for controlling GIN infection, the enhancement of host resistance through the selection of resistant animals has been suggested by many authors. Because of the difficulty of routinely collecting phenotypic indicators of parasite resistance, information derived from molecular markers may be used to improve the efficiency of classical genetic breeding.</p> <p>Methods</p> <p>A total of 181 microsatellite markers evenly distributed along the 26 sheep autosomes were used in a genome scan analysis performed in a commercial population of Spanish Churra sheep to detect chromosomal regions associated with parasite resistance. Following a daughter design, we analysed 322 ewes distributed in eight half-sib families. The phenotypes studied included two faecal egg counts (<it>LFEC0 </it>and <it>LFEC1</it>), anti-<it>Teladorsagia circumcincta </it>LIV IgA levels (<it>IgA</it>) and serum pepsinogen levels (<it>Peps</it>).</p> <p>Results</p> <p>The regression analysis revealed one QTL at the 5% genome-wise significance level on chromosome 6 for <it>LFEC1 </it>within the marker interval <it>BM4621-CSN3</it>. This QTL was found to be segregating in three out of the eight families analysed. Four other QTL were identified at the 5% chromosome-wise level on chromosomes 1, 10 and 14. Three of these QTL influenced faecal egg count, and the other one had an effect on <it>IgA </it>levels.</p> <p>Conclusion</p> <p>This study has successfully identified segregating QTL for parasite resistance traits in a commercial population. For some of the QTL detected, we have identified interesting coincidences with QTL previously reported in sheep, although most of those studies have been focused on young animals. Some of these coincidences might indicate that some common underlying loci affect parasite resistance traits in different sheep breeds. The identification of new QTL may suggest the existence of complex host-parasite relationships that have unique features depending on the host-parasite combination, perhaps due to the different mechanisms underlying resistance in adult sheep (hypersensitivity reactions) and lambs (immunity). The most significant QTL identified on chromosome 6 for <it>LFEC</it><sub>1 </sub>may be the target for future fine-mapping research efforts.</p>http://www.gsejournal.org/content/41/1/46 |
| spellingShingle | Primitivo Fermin San Meana Aranzazu Bayón Yolanda de la Fuente Luis-Fernando Martínez-Valladares Maria Álvarez Lorena Pérez Jorge Gutiérrez-Gil Beatriz Rojo-Vázquez Francisco-Antonio Arranz Juan-José Quantitative trait loci for resistance to trichostrongylid infection in Spanish Churra sheep Genetics Selection Evolution |
| title | Quantitative trait loci for resistance to trichostrongylid infection in Spanish Churra sheep |
| title_full | Quantitative trait loci for resistance to trichostrongylid infection in Spanish Churra sheep |
| title_fullStr | Quantitative trait loci for resistance to trichostrongylid infection in Spanish Churra sheep |
| title_full_unstemmed | Quantitative trait loci for resistance to trichostrongylid infection in Spanish Churra sheep |
| title_short | Quantitative trait loci for resistance to trichostrongylid infection in Spanish Churra sheep |
| title_sort | quantitative trait loci for resistance to trichostrongylid infection in spanish churra sheep |
| url | http://www.gsejournal.org/content/41/1/46 |
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