The elemental defense effect of cadmium on Alternaria brassicicola in Brassica juncea
Abstract Background The elemental defense hypothesis states a new defensive strategy that hyperaccumulators defense against herbivores or pathogens attacks by accumulating heavy metals. Brassica juncea has an excellent ability of cadmium (Cd) accumulation. However, the elemental defense effect and i...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-01-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-021-03398-4 |
| _version_ | 1818750312875294720 |
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| author | Zhe Liu Zhenzhen Sun Chaozhen Zeng Xujie Dong Mei Li Zhixiang Liu Mingli Yan |
| author_facet | Zhe Liu Zhenzhen Sun Chaozhen Zeng Xujie Dong Mei Li Zhixiang Liu Mingli Yan |
| author_sort | Zhe Liu |
| collection | DOAJ |
| description | Abstract Background The elemental defense hypothesis states a new defensive strategy that hyperaccumulators defense against herbivores or pathogens attacks by accumulating heavy metals. Brassica juncea has an excellent ability of cadmium (Cd) accumulation. However, the elemental defense effect and its regulation mechanism in B. juncea remain unclear. Results In this study, we profiled the elemental defense effect and the molecular regulatory mechanism in Cd-accumulated B. juncea after Alternaria brassicicola infection. B. juncea treated with 180 mg Kg− 1 DW CdCl2 2.5H2O exhibited obvious elemental defense effect after 72 h of infection with A. brassicicola. The expression of some defense-related genes including BjNPR1, BjPR12, BjPR2, and stress-related miRNAs (miR156, miR397, miR398a, miR398b/c, miR408, miR395a, miR395b, miR396a, and miR396b) were remarkably elevated during elemental defense in B. juncea. Conclusions The results indicate that Cd-accumulated B. juncea may defend against pathogens by coordinating salicylic acid (SA) and jasmonic acid (JA) mediated systemic acquired resistance (SAR) and elemental defense in a synergistic joint effect. Furthermore, the expression of miRNAs related to heavy metal stress response and disease resistance may regulate the balance between pathogen defense and heavy metal stress-responsive in B. juncea. The findings provide experimental evidence for the elemental defense hypothesis in plants from the perspectives of phytohormones, defense-related genes, and miRNAs. |
| first_indexed | 2024-12-18T04:17:41Z |
| format | Article |
| id | doaj.art-2426f91dc4d54102b0e6f8bf9a2a696f |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-18T04:17:41Z |
| publishDate | 2022-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-2426f91dc4d54102b0e6f8bf9a2a696f2022-12-21T21:21:19ZengBMCBMC Plant Biology1471-22292022-01-0122111410.1186/s12870-021-03398-4The elemental defense effect of cadmium on Alternaria brassicicola in Brassica junceaZhe Liu0Zhenzhen Sun1Chaozhen Zeng2Xujie Dong3Mei Li4Zhixiang Liu5Mingli Yan6Hunan Provincial Key Laboratory of Forestry Biotechnology, College of Life Science and Technology, Central South University of Forestry and TechnologyHunan Provincial Key Laboratory of Forestry Biotechnology, College of Life Science and Technology, Central South University of Forestry and TechnologyHunan Provincial Key Laboratory of Forestry Biotechnology, College of Life Science and Technology, Central South University of Forestry and TechnologyHunan Provincial Key Laboratory of Forestry Biotechnology, College of Life Science and Technology, Central South University of Forestry and TechnologyCrop Research Institute, Hunan Academy of Agricultural SciencesHunan Provincial Key Laboratory of Forestry Biotechnology, College of Life Science and Technology, Central South University of Forestry and TechnologyCrop Research Institute, Hunan Academy of Agricultural SciencesAbstract Background The elemental defense hypothesis states a new defensive strategy that hyperaccumulators defense against herbivores or pathogens attacks by accumulating heavy metals. Brassica juncea has an excellent ability of cadmium (Cd) accumulation. However, the elemental defense effect and its regulation mechanism in B. juncea remain unclear. Results In this study, we profiled the elemental defense effect and the molecular regulatory mechanism in Cd-accumulated B. juncea after Alternaria brassicicola infection. B. juncea treated with 180 mg Kg− 1 DW CdCl2 2.5H2O exhibited obvious elemental defense effect after 72 h of infection with A. brassicicola. The expression of some defense-related genes including BjNPR1, BjPR12, BjPR2, and stress-related miRNAs (miR156, miR397, miR398a, miR398b/c, miR408, miR395a, miR395b, miR396a, and miR396b) were remarkably elevated during elemental defense in B. juncea. Conclusions The results indicate that Cd-accumulated B. juncea may defend against pathogens by coordinating salicylic acid (SA) and jasmonic acid (JA) mediated systemic acquired resistance (SAR) and elemental defense in a synergistic joint effect. Furthermore, the expression of miRNAs related to heavy metal stress response and disease resistance may regulate the balance between pathogen defense and heavy metal stress-responsive in B. juncea. The findings provide experimental evidence for the elemental defense hypothesis in plants from the perspectives of phytohormones, defense-related genes, and miRNAs.https://doi.org/10.1186/s12870-021-03398-4MiRNAsSystemic acquired resistance (SAR)Defense-related genesCadmium |
| spellingShingle | Zhe Liu Zhenzhen Sun Chaozhen Zeng Xujie Dong Mei Li Zhixiang Liu Mingli Yan The elemental defense effect of cadmium on Alternaria brassicicola in Brassica juncea BMC Plant Biology MiRNAs Systemic acquired resistance (SAR) Defense-related genes Cadmium |
| title | The elemental defense effect of cadmium on Alternaria brassicicola in Brassica juncea |
| title_full | The elemental defense effect of cadmium on Alternaria brassicicola in Brassica juncea |
| title_fullStr | The elemental defense effect of cadmium on Alternaria brassicicola in Brassica juncea |
| title_full_unstemmed | The elemental defense effect of cadmium on Alternaria brassicicola in Brassica juncea |
| title_short | The elemental defense effect of cadmium on Alternaria brassicicola in Brassica juncea |
| title_sort | elemental defense effect of cadmium on alternaria brassicicola in brassica juncea |
| topic | MiRNAs Systemic acquired resistance (SAR) Defense-related genes Cadmium |
| url | https://doi.org/10.1186/s12870-021-03398-4 |
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