Introducing Wound Healing Assays in the Undergraduate Biology Laboratory Using Ibidi Plates
ABSTRACT The wound healing assay is a simple and inexpensive method that allows researchers to experimentally mimic cell growth and migration leading to wound healing. In this assay, a wound is created on a monolayer of cultured mammalian cells and cell migration is monitored. Micrographs are captur...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2022-08-01
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| Series: | Journal of Microbiology & Biology Education |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/jmbe.00061-22 |
| _version_ | 1811188254561533952 |
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| author | Yewseok K. Suh Ashley Robinson Nicholas Zanghi Austin Kratz Andrew Gustetic Mackenzie M. Crow Taylor Ritts William Hankey Verónica A. Segarra |
| author_facet | Yewseok K. Suh Ashley Robinson Nicholas Zanghi Austin Kratz Andrew Gustetic Mackenzie M. Crow Taylor Ritts William Hankey Verónica A. Segarra |
| author_sort | Yewseok K. Suh |
| collection | DOAJ |
| description | ABSTRACT The wound healing assay is a simple and inexpensive method that allows researchers to experimentally mimic cell growth and migration leading to wound healing. In this assay, a wound is created on a monolayer of cultured mammalian cells and cell migration is monitored. Micrographs are captured at regular intervals during the duration of the experiment. These microscopy images are analyzed to compare cell migration and wound closure under different conditions. Introduction of different cytotoxic treatments into a wound healing assay can provide information as to whether a particular drug or compound of interest has the ability to affect cell migration. This type of analysis is important when assessing the ability of a particular cancer cell line to display invasive and metastatic behaviors. One of the challenges of this assay is to create the original wound in a way that is consistent across plates or treatments, facilitating comparisons across experimental groups. This is a particular challenge when using the wound healing assay in the context of an undergraduate biology class to expose students to a distinct form of mammalian cell culture and help them apply scientific knowledge and research skills. We found an easy way to overcome this obstacle by using ibidi plates. In this article, we provide a simple protocol to use ibidi plates and HeLa cells to set up wound healing assays. This laboratory exercise allows undergraduate students to utilize different skills developed through cell culture experience, such as growing, treating, and imaging mammalian cells. |
| first_indexed | 2024-04-11T14:17:31Z |
| format | Article |
| id | doaj.art-242e8e4dfb5842bcad3a2e501314cb37 |
| institution | Directory Open Access Journal |
| issn | 1935-7877 1935-7885 |
| language | English |
| last_indexed | 2024-04-11T14:17:31Z |
| publishDate | 2022-08-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Journal of Microbiology & Biology Education |
| spelling | doaj.art-242e8e4dfb5842bcad3a2e501314cb372022-12-22T04:19:11ZengAmerican Society for MicrobiologyJournal of Microbiology & Biology Education1935-78771935-78852022-08-0123210.1128/jmbe.00061-22Introducing Wound Healing Assays in the Undergraduate Biology Laboratory Using Ibidi PlatesYewseok K. Suh0Ashley Robinson1Nicholas Zanghi2Austin Kratz3Andrew Gustetic4Mackenzie M. Crow5Taylor Ritts6William Hankey7Verónica A. Segarra8Department of Biology, High Point University, High Point, North Carolina, USADepartment of Biology, High Point University, High Point, North Carolina, USADepartment of Biology, High Point University, High Point, North Carolina, USADepartment of Chemistry, High Point University, High Point, North Carolina, USADepartment of Biology, High Point University, High Point, North Carolina, USADepartment of Biology, High Point University, High Point, North Carolina, USADepartment of Biology, High Point University, High Point, North Carolina, USADepartment of Genetics, University of North Carolina–Chapel Hill, Chapel Hill, North Carolina, USADepartment of Biology, High Point University, High Point, North Carolina, USAABSTRACT The wound healing assay is a simple and inexpensive method that allows researchers to experimentally mimic cell growth and migration leading to wound healing. In this assay, a wound is created on a monolayer of cultured mammalian cells and cell migration is monitored. Micrographs are captured at regular intervals during the duration of the experiment. These microscopy images are analyzed to compare cell migration and wound closure under different conditions. Introduction of different cytotoxic treatments into a wound healing assay can provide information as to whether a particular drug or compound of interest has the ability to affect cell migration. This type of analysis is important when assessing the ability of a particular cancer cell line to display invasive and metastatic behaviors. One of the challenges of this assay is to create the original wound in a way that is consistent across plates or treatments, facilitating comparisons across experimental groups. This is a particular challenge when using the wound healing assay in the context of an undergraduate biology class to expose students to a distinct form of mammalian cell culture and help them apply scientific knowledge and research skills. We found an easy way to overcome this obstacle by using ibidi plates. In this article, we provide a simple protocol to use ibidi plates and HeLa cells to set up wound healing assays. This laboratory exercise allows undergraduate students to utilize different skills developed through cell culture experience, such as growing, treating, and imaging mammalian cells.https://journals.asm.org/doi/10.1128/jmbe.00061-22wound healing assayibidi platescytotoxic agentsHeLa cellscancer invasion |
| spellingShingle | Yewseok K. Suh Ashley Robinson Nicholas Zanghi Austin Kratz Andrew Gustetic Mackenzie M. Crow Taylor Ritts William Hankey Verónica A. Segarra Introducing Wound Healing Assays in the Undergraduate Biology Laboratory Using Ibidi Plates Journal of Microbiology & Biology Education wound healing assay ibidi plates cytotoxic agents HeLa cells cancer invasion |
| title | Introducing Wound Healing Assays in the Undergraduate Biology Laboratory Using Ibidi Plates |
| title_full | Introducing Wound Healing Assays in the Undergraduate Biology Laboratory Using Ibidi Plates |
| title_fullStr | Introducing Wound Healing Assays in the Undergraduate Biology Laboratory Using Ibidi Plates |
| title_full_unstemmed | Introducing Wound Healing Assays in the Undergraduate Biology Laboratory Using Ibidi Plates |
| title_short | Introducing Wound Healing Assays in the Undergraduate Biology Laboratory Using Ibidi Plates |
| title_sort | introducing wound healing assays in the undergraduate biology laboratory using ibidi plates |
| topic | wound healing assay ibidi plates cytotoxic agents HeLa cells cancer invasion |
| url | https://journals.asm.org/doi/10.1128/jmbe.00061-22 |
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