Proteomics Methodologies: The Search of Protein Biomarkers Using Microfluidic Systems Coupled to Mass Spectrometry
Protein biomarkers have been the subject of intensive studies as a target for disease diagnostics and monitoring. Indeed, biomarkers have been extensively used for personalized medicine. In biological samples, these biomarkers are most often present in low concentrations masked by a biologically com...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Proteomes |
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| Online Access: | https://www.mdpi.com/2227-7382/11/2/19 |
| _version_ | 1797592897537703936 |
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| author | Isabel De Figueiredo Bernard Bartenlian Guillaume Van der Rest Antoine Pallandre Frédéric Halgand |
| author_facet | Isabel De Figueiredo Bernard Bartenlian Guillaume Van der Rest Antoine Pallandre Frédéric Halgand |
| author_sort | Isabel De Figueiredo |
| collection | DOAJ |
| description | Protein biomarkers have been the subject of intensive studies as a target for disease diagnostics and monitoring. Indeed, biomarkers have been extensively used for personalized medicine. In biological samples, these biomarkers are most often present in low concentrations masked by a biologically complex proteome (e.g., blood) making their detection difficult. This complexity is further increased by the needs to detect proteoforms and proteome complexity such as the dynamic range of compound concentrations. The development of techniques that simultaneously pre-concentrate and identify low-abundance biomarkers in these proteomes constitutes an avant-garde approach to the early detection of pathologies. Chromatographic-based methods are widely used for protein separation, but these methods are not adapted for biomarker discovery, as they require complex sample handling due to the low biomarker concentration. Therefore, microfluidics devices have emerged as a technology to overcome these shortcomings. In terms of detection, mass spectrometry (MS) is the standard analytical tool given its high sensitivity and specificity. However, for MS, the biomarker must be introduced as pure as possible in order to avoid chemical noise and improve sensitivity. As a result, microfluidics coupled with MS has become increasingly popular in the field of biomarker discovery. This review will show the different approaches to protein enrichment using miniaturized devices and the importance of their coupling with MS. |
| first_indexed | 2024-03-11T01:59:39Z |
| format | Article |
| id | doaj.art-242eb42c53a941c5babe031917de17b8 |
| institution | Directory Open Access Journal |
| issn | 2227-7382 |
| language | English |
| last_indexed | 2024-03-11T01:59:39Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Proteomes |
| spelling | doaj.art-242eb42c53a941c5babe031917de17b82023-11-18T12:20:05ZengMDPI AGProteomes2227-73822023-05-011121910.3390/proteomes11020019Proteomics Methodologies: The Search of Protein Biomarkers Using Microfluidic Systems Coupled to Mass SpectrometryIsabel De Figueiredo0Bernard Bartenlian1Guillaume Van der Rest2Antoine Pallandre3Frédéric Halgand4Institut de Chimie Physique, Université Paris Saclay, Avenue Jean Perrin, F91400 Orsay, FranceCentre des Nanosciences et Nanotechnologies, Université Paris Saclay, 10 Boulevard Thomas Gobert, F91120 Palaiseau, FranceInstitut de Chimie Physique, Université Paris Saclay, Avenue Jean Perrin, F91400 Orsay, FranceInstitut de Chimie Physique, Université Paris Saclay, Avenue Jean Perrin, F91400 Orsay, FranceInstitut de Chimie Physique, Université Paris Saclay, Avenue Jean Perrin, F91400 Orsay, FranceProtein biomarkers have been the subject of intensive studies as a target for disease diagnostics and monitoring. Indeed, biomarkers have been extensively used for personalized medicine. In biological samples, these biomarkers are most often present in low concentrations masked by a biologically complex proteome (e.g., blood) making their detection difficult. This complexity is further increased by the needs to detect proteoforms and proteome complexity such as the dynamic range of compound concentrations. The development of techniques that simultaneously pre-concentrate and identify low-abundance biomarkers in these proteomes constitutes an avant-garde approach to the early detection of pathologies. Chromatographic-based methods are widely used for protein separation, but these methods are not adapted for biomarker discovery, as they require complex sample handling due to the low biomarker concentration. Therefore, microfluidics devices have emerged as a technology to overcome these shortcomings. In terms of detection, mass spectrometry (MS) is the standard analytical tool given its high sensitivity and specificity. However, for MS, the biomarker must be introduced as pure as possible in order to avoid chemical noise and improve sensitivity. As a result, microfluidics coupled with MS has become increasingly popular in the field of biomarker discovery. This review will show the different approaches to protein enrichment using miniaturized devices and the importance of their coupling with MS.https://www.mdpi.com/2227-7382/11/2/19pre-treatment samplebiomarkerschromatographymicrofluidicsmass spectrometry (MS) |
| spellingShingle | Isabel De Figueiredo Bernard Bartenlian Guillaume Van der Rest Antoine Pallandre Frédéric Halgand Proteomics Methodologies: The Search of Protein Biomarkers Using Microfluidic Systems Coupled to Mass Spectrometry Proteomes pre-treatment sample biomarkers chromatography microfluidics mass spectrometry (MS) |
| title | Proteomics Methodologies: The Search of Protein Biomarkers Using Microfluidic Systems Coupled to Mass Spectrometry |
| title_full | Proteomics Methodologies: The Search of Protein Biomarkers Using Microfluidic Systems Coupled to Mass Spectrometry |
| title_fullStr | Proteomics Methodologies: The Search of Protein Biomarkers Using Microfluidic Systems Coupled to Mass Spectrometry |
| title_full_unstemmed | Proteomics Methodologies: The Search of Protein Biomarkers Using Microfluidic Systems Coupled to Mass Spectrometry |
| title_short | Proteomics Methodologies: The Search of Protein Biomarkers Using Microfluidic Systems Coupled to Mass Spectrometry |
| title_sort | proteomics methodologies the search of protein biomarkers using microfluidic systems coupled to mass spectrometry |
| topic | pre-treatment sample biomarkers chromatography microfluidics mass spectrometry (MS) |
| url | https://www.mdpi.com/2227-7382/11/2/19 |
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