Characterization and Comparison of Ocular Surface Microbiome in Newborns
The ocular microbiome is of fundamental importance for immune eye homeostasis, and its alteration would lead to an impairment of ocular functionality. Little evidence is reported on the composition of the ocular microbiota of term infants and on the impact of antibiotic prophylaxis. Methods: A total...
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MDPI AG
2022-07-01
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author | Francesco Petrillo Arianna Petrillo Maddalena Marrapodi Carlo Capristo Maria Francesca Gicchino Paolo Montaldo Elisabetta Caredda Michele Reibaldi Lara M. V. Boatti Federica Dell’Annunziata Veronica Folliero Marilena Galdiero |
author_facet | Francesco Petrillo Arianna Petrillo Maddalena Marrapodi Carlo Capristo Maria Francesca Gicchino Paolo Montaldo Elisabetta Caredda Michele Reibaldi Lara M. V. Boatti Federica Dell’Annunziata Veronica Folliero Marilena Galdiero |
author_sort | Francesco Petrillo |
collection | DOAJ |
description | The ocular microbiome is of fundamental importance for immune eye homeostasis, and its alteration would lead to an impairment of ocular functionality. Little evidence is reported on the composition of the ocular microbiota of term infants and on the impact of antibiotic prophylaxis. Methods: A total of 20 conjunctival swabs were collected from newborns at birth and after antibiotic treatment. Samples were subjected to 16S rRNA sequencing via system MiSeq Illumina. The data were processed with the MicrobAT software and statistical analysis were performed using two-way ANOVA. Results: Antibiotic prophylaxis with gentamicin altered the composition of the microbiota. In detail, a 1.5- and 2.01-fold reduction was recorded for <i>Cutibacterium acnes</i> (<i>C. acnes</i>) and <i>Massilia timonae</i> (<i>M. timonae</i>), respectively, whereas an increase in <i>Staphylococcus</i> spp. of 6.5 times occurred after antibiotic exposure. Conclusions: Antibiotic prophylaxis altered the ocular microbiota whose understanding could avoid adverse effects on eye health. |
first_indexed | 2024-03-09T13:20:06Z |
format | Article |
id | doaj.art-242fb473c92d47fbb01c6b5557eda25d |
institution | Directory Open Access Journal |
issn | 2076-2607 |
language | English |
last_indexed | 2024-03-09T13:20:06Z |
publishDate | 2022-07-01 |
publisher | MDPI AG |
record_format | Article |
series | Microorganisms |
spelling | doaj.art-242fb473c92d47fbb01c6b5557eda25d2023-11-30T21:30:41ZengMDPI AGMicroorganisms2076-26072022-07-01107139010.3390/microorganisms10071390Characterization and Comparison of Ocular Surface Microbiome in NewbornsFrancesco Petrillo0Arianna Petrillo1Maddalena Marrapodi2Carlo Capristo3Maria Francesca Gicchino4Paolo Montaldo5Elisabetta Caredda6Michele Reibaldi7Lara M. V. Boatti8Federica Dell’Annunziata9Veronica Folliero10Marilena Galdiero11Department of Surgical Sciences, Eye Clinic Section, University of Turin, 10124 Turin, ItalyPediatric Unit, Fondazione IRCCS “Ca’ Granda-Ospedale Maggiore-Policlinico”, 20122 Milan, ItalyDepartment of Woman, Child and General and Specialist Surgery, University of Campania “Luigi Vanvitelli”, 80138 Napoli, ItalyDepartment of Neonatology, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Woman, Child and General and Specialist Surgery, University of Campania “Luigi Vanvitelli”, 80138 Napoli, ItalyDepartment of Neonatology, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Neonatology, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Surgical Sciences, Eye Clinic Section, University of Turin, 10124 Turin, ItalyArrow Diagnostics, 16152 Genoa, ItalyDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyThe ocular microbiome is of fundamental importance for immune eye homeostasis, and its alteration would lead to an impairment of ocular functionality. Little evidence is reported on the composition of the ocular microbiota of term infants and on the impact of antibiotic prophylaxis. Methods: A total of 20 conjunctival swabs were collected from newborns at birth and after antibiotic treatment. Samples were subjected to 16S rRNA sequencing via system MiSeq Illumina. The data were processed with the MicrobAT software and statistical analysis were performed using two-way ANOVA. Results: Antibiotic prophylaxis with gentamicin altered the composition of the microbiota. In detail, a 1.5- and 2.01-fold reduction was recorded for <i>Cutibacterium acnes</i> (<i>C. acnes</i>) and <i>Massilia timonae</i> (<i>M. timonae</i>), respectively, whereas an increase in <i>Staphylococcus</i> spp. of 6.5 times occurred after antibiotic exposure. Conclusions: Antibiotic prophylaxis altered the ocular microbiota whose understanding could avoid adverse effects on eye health.https://www.mdpi.com/2076-2607/10/7/1390ocular surface microbiota16S rRNA sequencingnewbornbacteria |
spellingShingle | Francesco Petrillo Arianna Petrillo Maddalena Marrapodi Carlo Capristo Maria Francesca Gicchino Paolo Montaldo Elisabetta Caredda Michele Reibaldi Lara M. V. Boatti Federica Dell’Annunziata Veronica Folliero Marilena Galdiero Characterization and Comparison of Ocular Surface Microbiome in Newborns Microorganisms ocular surface microbiota 16S rRNA sequencing newborn bacteria |
title | Characterization and Comparison of Ocular Surface Microbiome in Newborns |
title_full | Characterization and Comparison of Ocular Surface Microbiome in Newborns |
title_fullStr | Characterization and Comparison of Ocular Surface Microbiome in Newborns |
title_full_unstemmed | Characterization and Comparison of Ocular Surface Microbiome in Newborns |
title_short | Characterization and Comparison of Ocular Surface Microbiome in Newborns |
title_sort | characterization and comparison of ocular surface microbiome in newborns |
topic | ocular surface microbiota 16S rRNA sequencing newborn bacteria |
url | https://www.mdpi.com/2076-2607/10/7/1390 |
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